In-service bridges along rivers or seas are constantly exposed to harsh environmental conditions, which contain corrosive substances that seriously affect the structural safety of these bridges. In its service processes, cyclic stress accelerates this corrosive effect, which aggravates fatigue and results in the failure of the materials utilized. Consequently, the expected endurance life is not reached. In this study, six test beams were designed, produced, and subsequently subjected to a chloride salt corrosion test to explore the effects of environment corrosion on the fatigue performance of bridge structures and reveal the development process of fatigue failure and its influencing factors. Fatigue amplitude was determined through comprehensive static and expressway vehicle load tests. Fatigue tests were also conducted on corrosion test beams, and the material properties of the pre-stressed concrete bridge after corrosion were analyzed under the effect of long-term traffic load. Fatigue brittle failure occurred in the form of reinforcement fracture in all test beams after corrosion. The service life of test beams with corrosion rates of 5% and 10% decreased by 53% and 96%, respectively, compared with that of a non-corroded beam at a fixed load level. Residual strain of the test beam with a corrosion rate of 10% under failure was twice that of the test beam with a corrosion rate of 5%. The maximum and residual strain curves of all test beams presented "three-phased" characteristics. After corrosion, the residual concrete strain in the compression zone and the frequency of the fatigue tests exhibited a double-logarithmic linear relation. Strain variation in the prestressed reinforcement after corrosion was significant, but the strain variation in the ordinary reinforcement was minimal. The analysis process and method of this study are also applicable to concrete members in other corrosive environments. The findings can provide a theoretical reference for fatigue performance analyses under different corrosion conditions.