Load cycling enhances bioactivity at the resin–dentin interface

Toledano, Manuel; Aguilera, Fátima S.; Sauro, Salvatore; Cabello, Inmaculada; Osorio, Estrella; Osorio, Raquel

doi:10.1016/j.dental.2014.02.009

Cited by 41 publications

(52 citation statements)

References 47 publications

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“…This difference also denotes a greater sensitivity to molecular orientation in order to enhance further crystallization. From a clinical standpoint, recovery [51] , better organization [52] , and collagen quality [53] [34] followed similar trend (Table 4). The amide A band, at 3396 cm -1 showed a 2.15 cm -1 (sound dentin) and 7.05 cm -1 (caries-affected dentin) shift to high frequencies, respectively, when Zn-containing amalgams were employed (Table 4).…”

Section: Resultsmentioning

confidence: 74%

Ex vivo detection and characterization of remineralized carious dentin, by nanoindentation and single point Raman spectroscopy, after amalgam restoration

Osorio

Aguilera

Cabello

et al. 2016

J Raman Spectroscopy

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The aim of this study was to assess the mechanical and chemical performance of sound and caries-affected dentin (CAD), after Zn-free vs containing amalgam restorations placement and thermocycling. Dentin surfaces were studied by Atomic Force Microscopy (AFM) analysis for surface morphological characterization (including fibril diameter assessment), nano-indentation (to measure nano-hardness-Hi and modulus of Young-Ei), and single point Raman spectroscopy for chemical analysis. Measurements were performed before amalgam placement, after amalgam removal, and after 3 months of thermocycling (100,000cy/5 ºC and 55 ºC). Restorations increased both Hi and Ei at intertubular dentin of CAD. The highest values of Hi were achieved at intertubular dentin after restoring with Zn-containing amalgams. Remineralization of dentin was attributed to the increase of both amorphous and crystalline new mineral, as lower degrees of crystal imperfections in junction with crystal disorders, and improvement in structural stability of collagen were found. Higher presence of minerals were also confirmed after the decrease of fluoridated apatite and the increase of the total water content. Proteoglycans, lipids, and proteins, augmented in both sound and CAD, providing support for the mineral growing. The increase of bands assigned to vibration of carbonate calcium phosphate contributed to a decrease of crystallinity.

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Section: Resultsmentioning

confidence: 74%

Ex vivo detection and characterization of remineralized carious dentin, by nanoindentation and single point Raman spectroscopy, after amalgam restoration

Osorio

Aguilera

Cabello

et al. 2016

J Raman Spectroscopy

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“…that showed a significant loss of intensity after extended loading (3 w) ( Table IVa).These indexes concerned with the maximum relative degree of mineralization 34,35 . Intertubular and peritubular mineralization were observed on CAD surfaces restored with Zn-free amalgams after 24 h (Fig 5a) or 3 w (Fig 5b) of load cycling, but extended mechanical loading 49 promoted multiple mineralized formations localized over the total surface of dentin. On the contrary, SD restored with Zn-containing amalgam produced a completely mineralized substratum, where the mineral formations allowed a null display of the tubule entrances (Fig 5c). )…”

Section: Resultsmentioning

confidence: 97%

“…Zinc-based salts [phosphorous (P), calcium (Ca), and zinc (Zn)] were detected in the elemental analysis (EDX, spectrum 2), at intertubular dentin. The analysis of µXRD 2 profiles (Fig 6b) Additionally, extended load cycling 49 showed a reduced symmetry in undistorted trigonal planar carbonate in the crystal lattice, which caused two CO [3][4] peaks (Figs 2e, 4e). This splitting effect into two bands has concerned some changes in this spectral region with carbonate content, ascribed to either growth of a carbonate peak or changes in the phosphate peak in response to the presence of carbonate in the lattice, as the 1,071 cm…”

Section: Resultsmentioning

confidence: 99%

On modeling and nanoanalysis of caries-affected dentin surfaces restored with Zn-containing amalgam and in vitro oral function

et al. 2015

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The aim of this research was to assess the influence of mechanical loading on the ability of Zn-free vs Zn-containing amalgams to promote remineralization at the dentin interface. Sound (SD) and caries-affected dentin surfaces (CAD) were restored using Zn-free or Zn-containing dental amalgams. Mid-coronal dentin surfaces were studied by 1) Atomic Force Microscopy (AFM) analysis (including plot and phase imaging, nanoindentation test (modulus of Young (Ei), nano-roughness measurements, and fibril diameter assessment), 2) Raman spectroscopy/cluster analysis, 3) X-ray diffraction (µXRD 2 ), 4) field emission electron microscope (FESEM) and energy-dispersive analysis (EDX), for morphological, mechanical, and physico-chemical characterization.Analyses were performed before amalgam placement and after amalgam removal, at 24 hours and 3 weeks of load cycling. Zn-free and Zn-containing amalgams restorations promoted an increase in the modulus of Young of CAD surfaces, after three weeks of load cycling; at this time, Zn-containing amalgams attained higher Ei than Zn-free restorations. Zn-containing amalgams induced tubular occlusion after load cycling, in both sound and CAD. Zn free-amalgams promoted remineralization of both intertubular and peritubular dentin in CAD substrata. These minerals were identified as calciumphosphate deposits, and crystals as hydroxyl-apatite with augmented crystallographic maturity but with some components of lattice distortion. Crosslinking of collagen diminished and secondary structure of collagen increased in CAD substrate restored with Zn-containing amalgam after 3 w of load cycling, indicating an advanced preservation, molecular organization and orientation of collagen fibrils after load cycling. Plot and phase images permitted to observe the topographical changes which were promoted by the mineral deposits; in general, the indexes related to higher remineralization gave rise to a decrease of nano-roughness and an augmentation of the 4 bandwidth of the collagen fibrils. Zn-containing amalgam restorations submitted to mechanical stimuli promote remineralization of the partially mineral-depleted subjacent substrate at the caries-affected dentin.

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“…Ortalama çiğneme frekansına en yakın değer olan 0.5 Hz' den 27 daha yüksek frekansların laboratuvarda çalıĢma süresini kısalttığı ancak örneklerde iç ısı artıĢına neden olabileceği belirtilmektediR. Literatürde kullanılan kuvvetler incelendiğinde sıklıkla 50 N' luk yük uygulamasının tercih edildiği görülmektedir 28 . Li ve ark.…”

Section: Loadingunclassified

Ağiz Ortaminin Si̇mülasyonu Açisindan Termal Ve Loading Si̇klusun Önemi̇

Atay¹,

Özel²

2015

Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

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Load cycling enhances bioactivity at the resin–dentin interface

Cited by 41 publications

References 47 publications

Ex vivo detection and characterization of remineralized carious dentin, by nanoindentation and single point Raman spectroscopy, after amalgam restoration

Ex vivo detection and characterization of remineralized carious dentin, by nanoindentation and single point Raman spectroscopy, after amalgam restoration

On modeling and nanoanalysis of caries-affected dentin surfaces restored with Zn-containing amalgam and in vitro oral function

Ağiz Ortaminin Si̇mülasyonu Açisindan Termal Ve Loading Si̇klusun Önemi̇

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