lncRNA2919 Suppresses Rabbit Dermal Papilla Cell Proliferation via trans-Regulatory Actions

Abstract

Hair follicles (HFs) are complex organs that grow cyclically during mammals’ growth and development. Long non-coding RNAs (lncRNAs) cannot be translated into proteins and play crucial roles in many biological processes. In our previous study, candidate lncRNAs associated with HF cyclic regeneration were screened, and we identified that the novel lncRNA, lncRNA2919, was significantly expressed during catagen. Here, we identified that lncRNA2919 has no coding potentiality and is highly expressed in the cell nucleus, and downregulates HF growth and development-related genes, inhibits cell proliferation, and promotes cell apoptosis in rabbit dermal papilla cells. lncRNA2919 recruits STAT1 to form a compound. As a key transcription factor, STAT1 regulates the transcriptional expression of KRTAP11-1. Our study revealed that lncRNA2919 is involved in HF cyclic regeneration through the trans-regulatory lncRNA2919–STAT1–KRTAP11-1 axis. This study elucidates the mechanism through which lncRNA2919 regulates HF growth and development and the role of lncRNA2919 as a new therapeutic target in animal wool production and human hair-related disease treatment.

DNA Methylation Mediates lncRNA2919 Regulation of Hair Follicle Regeneration

“…The PrimeScript 1st Strand cDNA Synthesis Kit (Takara, Dalian, China) was used for the synthesis of high-quality rabbit skin cDNA, and then PCR was performed using Phanta Max Super-Fidelity DNA Polymerase (Vazyme, Nanjing, China). Following the steps, the overexpression vectors of pcDNA3.1-EGR1 and pcDNA3.1-DNMT1 were constructed, and pcDNA3.1-lncRNA2919 was constructed using the full-length sequence of lncRNA2919 [ 46 ]. The short hairpin RNA (shRNA)-lncRNA2919 and small interfering RNA (siRNA)-EGR1 were designed and purchased from GenePharma Co., Ltd. (Shanghai, China).…”

The Function and Regulation Mechanism of Non-Coding RNAs in Muscle Development

“…The precursor miRNAs are exported from the nucleus to the cytoplasm and cleaved again by the Dicer enzyme to form miRNA double-stranded bodies containing Long noncoding RNA is a transcript that does not code for protein and is greater than 200 nucleotides in length [38]. Its sequence conservation and expression levels are lower than those of protein-coding genes, but a growing body of evidence confirms that it plays a key role in biological processes such as organ growth and development [39], cell proliferation and differentiation [40], glycolipid metabolism [41], and disease [42]. This is mainly due to the intrinsic nature of RNA molecules and their ability to form complex secondary structures that allow them to bind a wide range of molecules.…”

“…In addition, a large number of studies have also shown that these three RNAs are widely involved in various important life activities in animals, including organ growth and development, cell proliferation and differentiation, glycolipid metabolism, and disease aspects, etc. [39,[53][54][55].…”

Hair Follicle Development of Rex Rabbits Is Regulated Seasonally by Wnt10b/β-Catenin, TGFβ-BMP, IGF1, and EGF Signaling Pathways

“…Hair follicles rely on periodic cyclic growth to achieve hair growth and renewal, and their periodic changes determine the periodic growth and loss of hair, while the periodic development of hair follicles is affected by a variety of factors and is regulated by a series of signal molecules [9][10][11]. As the "material basis" of fur growth, hair follicles are not only regulated by genetic and nutritional factors but are also affected by environmental temperature and humidity [12][13][14][15]. Many studies have been conducted on animals under heat and cold stresses [16][17][18], while the effect of the seasons on fur rabbits has rarely been reported, and the mechanism of seasonal influence on hair follicle development remains unclear.…”