LNCRNA XIST Inhibits miR-377-3p to Hinder Th17 Cell Differentiation through Upregulating ETS1

Abstract

Background. Th17 cell differentiation is involved in the development and progression of many diseases, such as rheumatoid arthritis and systemic lupus erythematosus. Present study mainly focused on the role of LINC-XIST in Th17 cell differentiation. Methods. The naïve CD4+ T cells were isolated from human whole blood. Cells were cultured under Th17 cell-polarizing condition for 6 days. The expression of LINC-XIST and miR-153-3p was measured by qPCR. The relationship between LINC-XIST, miR-153-3p, and ETS1 was predicted by TargetScan website and authenticated by luciferase reporter assay. ELISA assays were conducted to evaluate the IL-17 concentration. Western blot was utilized to measure the protein expression of ETS1. Th17 cell frequency was examined by flow cytometry. Results. The expression of XIST markedly decreased and miR-153-3p expression markedly increased with Th17 cell differentiation. The mRNA expression of IL-17, IL-17 concentration, and Th17 cell frequency were observably decreased in overexpressed LINC-XIST group. Luciferase reporter assay authenticated that miR-153-5p was directly regulated by LINC-XIST. miR-153-3p inhibitor observably decreased IL-17 concentration, mRNA expression of IL-17, and Th17 cell frequency while si-XIST reversed this impact. ETS1 was confirmed to be regulated by miR-153-5p via luciferase reporter assay. In addition, ETS1 markedly decreased IL-17 mRNA expression, IL-17 concentration, and Th17 cell frequency while miR-153-5p mimic reversed this impact. Conclusion. LNCRNA XIST inhibited miR-377-3p to hinder Th17 cell differentiation through upregulating ETS1.

The Common LncRNAs of Neuroinflammation-Related Diseases

“…(Zheng et al, 2020) XIST inhibits Th17 cell differentiation through miR-377-3p/ETS1. (Yao et al, 2022) XIST is a potential diagnostic marker of triple-negative breast cancer, colorectal cancer, and acute myocardial infarction, and 53BP1 combined with XIST is a prognostic marker of BRCA1-like breast cancer. (Lan et al, 2021;Schouten et al, 2016;Yu et al, 2020a;Zheng et al, 2022) XIST plays a proinflammatory role in epilepsy and neuropathic pain by acting as a ceRNA of miRNA.…”

The emerging role of long non-coding RNAs in renal cell carcinoma progression and clinical therapy via targeting metabolic regulation

“…Although lncRNAs lack the ability of protein coding, they are still transcribed by RNA polymerase II, and then undergo posttranscription capping, splicing and polyadenylation ( Shen et al, 2021 ). The main functions of lncRNAs include X chromosome silencing, transcriptional activation, transcriptional interference, tumor regulation, and maintenance of RNA stability ( Yao et al, 2022 ). More and more evidence shows that highly structured lncRNAs bind to chromatin regulators and change their state, and lncRNAs participate in the occurrence of tumor and the expression disorder of protein coding genes in tumors by interacting with transcription factors, RNAs and miRNAs ( Rinn and Chang, 2012 ).…”

Retracted: LNCRNA XIST Inhibits miR-377-3p to Hinder Th17 Cell Differentiation through Upregulating ETS1

“…Computational Intelligence and Neuroscience has retracted the article titled “LNCRNA XIST Inhibits miR-377-3p to Hinder Th17 Cell Differentiation through Upregulating ETS1” [ 1 ] due to concerns that the peer review process has been compromised.…”