lncRNA uc009yby.1 promotes renal cell proliferation and is associated with poor survival in patients with clear cell renal cell carcinomas

Abstract

Abstract. The expression and function of long non-coding RNAs (lncRNAs) in clear cell renal cell carcinoma (ccRCC) remains unclear. The present study measured the expression profiles of three lncRNAs (uc009yby.1, ENST00000514034, and ENST00000450687) using reverse transcriptionquantitative polymerase chain reaction, and assessed their signatures in distinguishing ccRCC from matched normal tissues via analysis of receiver operating characteristic (ROC) curves. The expression of uc009yby.1 was inhibited by transfection of renal cells with small interfering RNA, and then the cell proliferation was evaluated by using a Cell Counting Kit-8. The results showed that the expressions of uc009yby.1 and ENST00000514034 were markedly increased in ccRCC compared with the matched normal tissues (P<0.0001 and P= 0.0008, respectively), whereas the ENST00000450687 expression was not significantly altered. ROC curves yielded an area under the curve (AUC) value of 0.7000 for uc009yby.1, with sensitivity of 54.29% and specificity of 82.86%; and an AUC value of 0.6627 for ENST00000514034, with sensitivity of 60.00% and specificity of 67.14%. Furthermore, knockdown of uc009yby.1 suppressed renal cell proliferation (Day 0, P= 0.7844; Day 1, P= 0.0018; Day 2, P= 0.0001; Day 3, P<0.000; Day 4, P<0.0001). Taken together, these findings suggest that the expression profiles of uc009yby.1 and ENST00000514034 may serve as novel biomarkers for ccRCC detection, and that uc009yby.1 is strongly associated with renal cell proliferation.

Long non-coding RNAs in renal cell carcinoma: A systematic review and clinical implications

“…In addition, eight lncRNAs transcribed from the loci (ACTN4, CSNK1D, DNAJC3, GIGYF2, HDAC5, PTPN3, RAB25, and VPS13B) were detected as altered in the expression profiles in both of the malignancy and the survival outcomes [ 42 ]. Furthermore, PVT1 was strongly overexpressed in ccRCC and associated with the enhancement of MYC signaling and poorer clinical outcome [ 57 ].…”

Downregulation of lncRNA SDPR-AS is associated with poor prognosis in renal cell carcinoma

“…Long ncRNAs (lncRNAs) are RNA molecules that are larger than 200 nucleotides in length and with no protein coding capabilities 6,7. Recently, many studies have shown that lncRNAs are involved in carcinogenesis, including invasion and metastasis of kidney, breast, and colorectal cancers 8–10. Particularly in RCC, it has been reported that high expression of lncRNA ATB promotes cell migration and invasion, and correlates with metastases,11 while low expression of lncRNA NBAT-1 increases cell proliferation, migration, and invasion, and is also associated with poor prognosis 12.…”

Renal Cell Tumors: Uncovering the Biomarker Potential of ncRNAs

“…In another study, comprising 102 ccRCC and 50 NRT, lncRNA CYP4A22-2/3 discriminated ccRCC from NRT with an AUC of 0.790 [177]. In 2016, the expression of lncRNA UC009YBY.1 and lncRNA ENST00000514034 was assessed in a set of 70 ccRCC and 70 MNT by Ren and colleagues [178]. These authors reported that the two lncRNAs could identify RCC tissue with 54.29% sensitivity and 82.86% specificity for the former, and 60.00% sensitivity and 67.14% specificity for the latter [178].…”

“…In 2016, the expression of lncRNA UC009YBY.1 and lncRNA ENST00000514034 was assessed in a set of 70 ccRCC and 70 MNT by Ren and colleagues [178]. These authors reported that the two lncRNAs could identify RCC tissue with 54.29% sensitivity and 82.86% specificity for the former, and 60.00% sensitivity and 67.14% specificity for the latter [178]. Finally, a recent study reported that lncRNA HOX Transcript Antisense RNA (HOTAIR) might also constitute a ccRCC diagnostic biomarker, disclosing an AUC of 0.9230 [71].…”