lncRNA TP73-AS1 Regulates miR-21/PTEN Axis to Affect Cell Proliferation in Acute Myeloid Leukemia

Yuan, Zhongtao; Li, Luqiong; Zheng, Mai; Xu, Jingtao; Wang, Wei

doi:10.1089/cbr.2019.3142

Cited by 11 publications

(7 citation statements)

References 17 publications

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“…The role of STAT3 signaling pathway in chemoresistance has been investigated (Shih, 2020). It has been demonstrated that inhibition of STAT3 by miR‐29b elevates the sensitivity of cancer cells into temozolomide chemotherapy (Yuan, Li, Zheng, Xu, & Wang, 2020). The inhibitor of DNA binding 1 (ID1) induces NF‐κB signaling pathway to elevate IL‐6 levels.…”

Section: Cryptotanshinone In Cancer Therapymentioning

confidence: 99%

Recent advances and future directions in anti‐tumor activity of cryptotanshinone: A mechanistic review

Ashrafizadeh

Zarrabi

Orouei

et al. 2020

Phytotherapy Research

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In respect to the enhanced incidence rate of cancer worldwide, studies have focused on cancer therapy using novel strategies. Chemotherapy is a common strategy in cancer therapy, but its adverse effects and chemoresistance have limited its efficacy. So, attempts have been directed towards minimally invasive cancer therapy using plant derived‐natural compounds. Cryptotanshinone (CT) is a component of salvia miltiorrihiza Bunge, well‐known as Danshen and has a variety of therapeutic and biological activities such as antioxidant, anti‐inflammatory, anti‐diabetic and neuroprotective. Recently, studies have focused on anti‐tumor activity of CT against different cancers. Notably, this herbal compound is efficient in cancer therapy by targeting various molecular signaling pathways. In the present review, we mechanistically describe the anti‐tumor activity of CT with an emphasis on molecular signaling pathways. Then, we evaluate the potential of CT in cancer immunotherapy and enhancing the efficacy of chemotherapy by sensitizing cancer cells into anti‐tumor activity of chemotherapeutic agents, and elevating accumulation of anti‐tumor drugs in cancer cells. Finally, we mention strategies to enhance the anti‐tumor activity of CT, for instance, using nanoparticles to provide targeted drug delivery.

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Section: Cryptotanshinone In Cancer Therapymentioning

confidence: 99%

Recent advances and future directions in anti‐tumor activity of cryptotanshinone: A mechanistic review

Ashrafizadeh

Zarrabi

Orouei

et al. 2020

Phytotherapy Research

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“…Moreover, it has been revealed that PTEN is functionally lost in human leukemia through deletion, mutation, transcriptional silencing, and protein inactivation 35 . Two recent studies reported that the expression of PTEN in AML cells could be regulated by long noncoding RNAs via sponging targeted miRNAs 36,37 . Here, we found that PTEN is a downstream target of miR‐516b.…”

Section: Discussionmentioning

confidence: 59%

Circular RNA circ_0040823 inhibits the proliferation of acute myeloid leukemia cells and induces apoptosis by regulating miR‐516b/PTEN

Wang

Yang

Jen

et al. 2022

The Journal of Gene Medicine

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Objective Endogenous circular RNAs (circRNAs) and microRNAs (miRNAs) have been shown to regulate the pathogenesis of acute myeloid leukemia (AML). The current study aimed to identify the role of circRNA 0040823 (circ_0040823) in AML. Methods Microarray datasets were analyzed to identify differentially expressed circRNAs in AML patients. Peripheral blood samples were obtained from healthy volunteers and AML patients for the measurement of circ_0040823 and miR‐516b levels. The overexpression or knockdown of a target gene in AML cells was achieved by the transfection with lentiviral vectors or small interfering RNAs. BALB/c nude mice were inoculated with AML cells and monitored for tumor growth. Dual‐luciferase reporter assay, RNA immunoprecipitation, and RNA pull‐down assay were used to determine the binding relationship between circRNA and miRNA. Results circ_0040823 was significantly downregulated in AML patients and leukemia cells. Overexpression of circ_0040823 inhibited AML cell proliferation, and induced apoptosis and cell cycle arrest. Upregulation of circ_0040823 also repressed the growth of xenograft tumors in vivo. circ_0040823 acted as a miR‐516b sponge and regulated key cellular events in leukemia cells via downregulating miR‐516b. Moreover, tumor suppressor phosphatase and tensin homolog (PTEN) was a downstream target of miR‐516b. The inhibition of miR‐516b impaired the proliferation capacity of leukemia cells and induced apoptosis, while PTEN deficiency attenuated these effects. Conclusion This study showed that circ_0040823 inhibited proliferation and induced apoptosis of AML cells by sponging miR‐516b, thereby diminishing the regulatory effect of miR‐516b on PTEN. These findings identified circ_0040823/miR‐516b/PTEN as a new therapeutic target for AML.

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“…LncRNA can be detected both in nucleus and cytoplasm, with the former acting on chromatin to regulate gene expression, and the latter acting on mRNA to regulate the translation process 25 . As a regulator of specific gene, lncRNA can exert its biological effects such as regulating epigenetics, controlling cell cycle, regulating differentiation and immune response by regulating transcription, translation, mRNA shearing, post‐transcriptional modification and other processes 26–28 . In this study, we found that ENSMUST00000147869 not only increases the degradation of protein Hspa9 through the ubiquitin proteasome pathway, but also reduces the combination of Hspa9 and HMGB1, thereby delaying the process of DN.…”

Section: Discussionmentioning

confidence: 69%

ENSMUST00000147869 regulates proliferation and fibrosis of mesangial cells in diabetic nephropathy by interacting with Hspa9

et al. 2022

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Our previous study showed that ENSMUST00000147869 was abnormally low expressed in the early stage of diabetic nephropathy (DN). ENSMUST000 00147869 could inhibit the fibrosis and proliferation of mouse mesangial cells (MMCs), but the mechanism is still unclear. This study aims to explore the specific mechanism underline ENSMUST00000147869 regulates the proliferation and fibrosis of MMCs in DN. Nucleocytoplasmic fractionation was applied to define the location of ENSMUST00000147869 in MMCs. RNA-protein pulldown, RNA immunoprecipitation and mass spectrometry were used to identify upregulated Hspa9 directly interacting with ENSMUST00000147869. SiRNA and lentivirus packaging were used to clarify the role of Hspa9 downregulated by ENSMUST00000147869 in promoting proliferation and fibrosis in MMCs. CHX and MG132 were used to clarify the regulatory role of ENSMU ST00000147869 to Hspa9. Immunoprecipitation confirmed the binding of Hspa9 and HMGB1. HSPA9 was a direct binding protein of ENSMU ST00000147869, and ENSMUST00000147869 could inhibit proliferation and fibrosis of MMCs by down-regulating HSPA9 through ubiquitination process. HMGB1 was the downstream binding protein of Hspa9, and ENSMUS T00000147869 could inhibit the interaction between Hspa9 and HMGB1. Our data showed that ENSMUST00000147869 regulates Hspa9 through the ubiquitin proteasome pathway and inhibits the binding of Hspa9 and HMGB1.The ENSMUST00000147869/Hspa9/HMGB1 axis may act as a diagnostic molecular marker and an effective therapeutic target for DN.

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lncRNA TP73-AS1 Regulates miR-21/PTEN Axis to Affect Cell Proliferation in Acute Myeloid Leukemia

Cited by 11 publications

References 17 publications

Recent advances and future directions in anti‐tumor activity of cryptotanshinone: A mechanistic review

Recent advances and future directions in anti‐tumor activity of cryptotanshinone: A mechanistic review

Circular RNA circ_0040823 inhibits the proliferation of acute myeloid leukemia cells and induces apoptosis by regulating miR‐516b/PTEN

ENSMUST00000147869 regulates proliferation and fibrosis of mesangial cells in diabetic nephropathy by interacting with Hspa9

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