LncRNA Tincr regulates PKCɛ expression in a miR-31-5p-dependent manner in cardiomyocyte hypertrophy

Li, Hao; Shi, Hongtao; Zhang, Fan; Xue, Honghong; Wang, Lei; Tian, Jing; Xu, Jianrong; Han, Qinghua

doi:10.1007/s00210-020-01847-9

Cited by 10 publications

(7 citation statements)

References 36 publications

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“…Moreover, it can influence the drug sensitivity and efficacy of chemotherapy in colorectal cancer and hepatocellular carcinoma cells (18,39). It has been considered as a target of long noncoding or circular RNA in cardiomyocyte hypertrophy and pre-eclampsia (40,41), and a shared regulator of chronic mucus hypersecretion in asthma and chronic obstructive pulmonary disease (42). In the present study, miR-31 was upregulated in SEOCRC and DMD was downregulated.…”

Section: Discussionsupporting

confidence: 48%

miR‑31‑5p‑DMD axis as a novel biomarker for predicting the development and prognosis of sporadic early‑onset colorectal cancer

Liu

Chang

et al. 2022

Oncol Lett

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The incidence of colorectal cancer (CRC) is increasing in young adults, but knowledge regarding the molecular features of sporadic early-onset colorectal cancer (SEOCRC) is limited. The objective of the present study was to investigate potential key tumorigenesis-associated genes and their regulatory microRNAs (miRNAs) in SEOCRC. Using miRNA and mRNA expression screening of SEOCRC and sporadic late-onset colorectal cancer (SLOCRC) by next generation sequencing (NGS) and bioinformatics, the SEOCRC-associated miRNAome and transcriptome were analyzed. In SEOCRC miRNA and mRNA expression profiles, the tumorigenesis-associated genes and their regulatory miRNAs were analyzed according to the miRTarBase database, and specific miRNA-mRNA pairs were selected as the candidate biomarkers in SEOCRC, which were further verified in another cohort of SEOCRC and SLOCRC patients' colon cancer and paracancerous tissues using reverse transcription-quantitative PCR and immunohistochemistry. Moreover, the clinical relevance of these paired signatures to clinicopathological features was determined in 80 patients with SEOCRC. The expression of dystrophin (DMD) was downregulated and that of miR-31-5p was upregulated in SEOCRC tissue compared with adjacent peritumoral tissue. While DMD and miR-31-5p were not differentially expressed in SLOCRC tissues compared with that in adjacent peritumoral tissues. The miR-31-5p-DMD axis was identified as the key regulatory axis specific to SEOCRC, and DMD expression was closely associated with TNM stage and lymph node metastasis. Importantly, Kaplan-Meier analysis revealed that patients with low DMD expression had significantly poorer overall survival, cancer specific survival and recurrence free survival compared with those with high expression of DMD.In conclusion, the miR-31-5p-DMD axis may serve as a novel biomarker in predicting the development of SEOCRC, and DMD can be used as a promising biomarker for the prognosis of SEOCRC.

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Section: Discussionsupporting

confidence: 48%

miR‑31‑5p‑DMD axis as a novel biomarker for predicting the development and prognosis of sporadic early‑onset colorectal cancer

Liu

Chang

et al. 2022

Oncol Lett

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“…Researchers have confirmed that PRKCE is a direct target of hsa-miR-31-5p. [38] It is a known tumor biomarker that plays important roles in different processes that lead to cancer development. [39] Therefore, the identified MIR99AHG-hsa-miR-31-5p- PRKCE axis may be critical for NSCLC and can be used as a tumor biomarker and novel therapeutic target for elderly patients with the aforementioned type of cancer.…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of competitive endogenous RNA networks in elder patients with non-small cell lung cancer

Chen

Zhu

et al. 2023

Medicine

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Background: Lung cancer is one of the most prevalent cancers and the leading cause of cancer-related deaths worldwide; non-small cell lung cancer (NSCLC) comprises approximately 80% of all lung cancer cases. This study aimed to construct a competing endogenous RNA (ceRNA) network and identify prognostic signatures in elderly patients with NSCLC. Methods: We extracted data from elderly patients with NSCLC from The Cancer Genome Atlas and identified differentially expressed (DE) messenger RNAs (mRNAs), microRNAs (miRNAs), and long non-coding RNAs (lncRNAs). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to investigate the functions of DEmRNAs. The interactions between RNAs were predicted using starBase, TargetScan, miRTarBase, and miRanda. Cytoscape version 3.0 was used to construct and visualize the lncRNA-miRNA-mRNA ceRNA network. The association between the expression levels of DERNAs in the constructed ceRNA network and overall survival was determined using the survival package in R software. Furthermore, another Gene Expression Omnibus cohort was studied to externally validate the ceRNA network. Results: In total, 2865 DEmRNAs, 62 DEmiRNAs, and 131 DElncRNAs were identified. Dysregulated mRNAs are enriched in cancer-related processes and pathways. A ceRNA network was constructed using 38 miRNAs, 61 lncRNAs, and 164 mRNAs. Of these, 3 lncRNAs, 3 miRNAs, and 16 mRNAs were closely related to overall survival. The MIR99AHG-hsa-miR-31-5p-PRKCE axis has been identified as a potential ceRNA network involved in the development of NSCLC in elderly individuals. External validation of the MIR99AHG-hsa-miR-31-5p-PRKCE axis in the GSE19804 cohort showed that PRKCE was downregulated and that MIR99AHG was upregulated in the tumor tissues of elderly patients with NSCLC compared with normal lung tissues. Conclusions: This study provides novel insights into the lncRNA-miRNA-mRNA ceRNA network and reveals potential biomarkers for the diagnosis and prognosis of elderly patients with NSCLC.

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“…In human atrial fibrillation, the increased level of miR-31 causes arrhythmia [ 23 ], and after myocardial infarction it induces detrimental cardiac remodeling [ 24 ]. Tincr-miR-31-5p axis targets PRKCE, which is involved in cardiomyocyte hypertrophy [ 25 ]. miR-31-5p/155 are upregulated in endothelial cells by inflammatory cytokines and inhibit the eNOS/NO axis, resulting in disruption of vascular homeostasis [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

miR-31-5p-Modified RAW 264.7 Macrophages Affect Profibrotic Phenotype of Lymphatic Endothelial Cells In Vitro

Moskalik

Ratajska

Majchrzak

et al. 2022

IJMS

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Cardiac lymphatic vessel (LyV) remodeling as a contributor to heart failure has not been extensively evaluated in metabolic syndrome (MetS). Our studies have shown structural changes in cardiac LyV in MetS that contribute to the development of edema and lead to myocardial fibrosis. Tissue macrophages may affect LyV via secretion of various substances, including noncoding RNAs. The aim of the study was to evaluate the influence of macrophages modified by miR-31-5p, a molecule that regulates fibrosis and lymphangiogenesis, on lymphatic endothelial cells (LECs) in vitro. The experiments were carried out on the RAW 264.7 macrophage cell line and primary dermal lymphatic endothelial cells. RAW 264.7 macrophages were transfected with miR-31-5p and supernatant from this culture was used for LEC stimulation. mRNA expression levels for genes associated with lymphangiogenesis and fibrosis were measured with qRT-PCR. Selected results were confirmed with ELISA or Western blotting. miR-31-5p-modified RAW 264.7 macrophages secreted increased amounts of VEGF-C and TGF-β and a decreased amount of IGF-1. The supernatant from miR-31-5p-modified RAW 264.7 downregulated the mRNA expression for genes regulating endothelial-to-mesenchymal transition (EndoMT) and fibrosis in LECs. Our results suggest that macrophages under the influence of miR-31-5p show the potential to inhibit LEC-dependent fibrosis. However, more studies are needed to confirm this effect in vivo.

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LncRNA Tincr regulates PKCɛ expression in a miR-31-5p-dependent manner in cardiomyocyte hypertrophy

Cited by 10 publications

References 36 publications

miR‑31‑5p‑DMD axis as a novel biomarker for predicting the development and prognosis of sporadic early‑onset colorectal cancer

miR‑31‑5p‑DMD axis as a novel biomarker for predicting the development and prognosis of sporadic early‑onset colorectal cancer

Integrated analysis of competitive endogenous RNA networks in elder patients with non-small cell lung cancer

miR-31-5p-Modified RAW 264.7 Macrophages Affect Profibrotic Phenotype of Lymphatic Endothelial Cells In Vitro

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