lncRNA TINCR facilities bladder cancer progression via regulating miR‑7 and mTOR

Xu, Gao; Yang, Hao; Liu, Meichun; Niu, Jintao; Chen, Weidong; Tan, Xiaojing; Sun, Li

doi:10.3892/mmr.2020.11530

Cited by 12 publications

(11 citation statements)

References 30 publications

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“…Through online analysis of prognostic data, we also found that patients with head and neck tumours with high expression of TINCR had a better prognosis. The overexpression of TINCR in the laryngeal cancer cell line TU212 significantly inhibited cancer cell proliferation, migration, and invasion, whereas TINCR knockdown promoted proliferation and invasion, which was consistent with research in bladder cancer [ 21 ]. The results showed that TINCR expression in LSCC tissue cells was significantly downregulated.…”

Section: Discussionsupporting

confidence: 87%

TINCR inhibits the proliferation and invasion of laryngeal squamous cell carcinoma by regulating miR-210/BTG2

Pang

Han

et al. 2021

BMC Cancer

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Background Terminal differentiation-induced ncRNA (TINCR) plays an essential role in epidermal differentiation and is involved in the development of various cancers. Methods qPCR was used to detect the expression level of TINCR in tissues and cell lines of laryngeal squamous cell carcinoma (LSCC). The potential targets of TINCR were predicted by the bioinformation website. The expression of miR-210 and BTG2 genes were detected by qPCR, and the protein levels of BTG2 and Ki-67 were evaluated by western blot. CCK-8 assay, scratch test, and transwell chamber were used to evaluate the proliferation, invasion, and metastasis ability of LSCC cells. The relationships among TINCR, miR-210, and BTG2 were investigated by bioinformatics software and luciferase reporter assay. The in vivo function of TINCR was accessed on survival rate and tumor growth in nude mice. Results We used qRT-PCR to detect the expression of TINCR in laryngeal squamous cell carcinoma (LSCC) tissues and cells and found significantly lower levels in cancer tissues compared with adjacent tissues. Additionally, patients with high TINCR expression had a better prognosis. TINCR overexpression was observed to inhibit the proliferation and invasion of LSCC cells. TINCR was shown to exert its antiproliferation and invasion effects by adsorbing miR-210, which significantly promoted the proliferation and invasion of laryngeal squamous cells. Overexpression of miR-210 was determined to reverse the tumour-suppressive effects of TINCR. BTG2 (anti-proliferation factor 2) was identified as the target gene of miR-210, and BTG2 overexpression inhibited the proliferation and invasion of LSCC cells. BTG2 knockdown relieved the inhibitory effects of TINCR on the proliferation and invasion of LSCC. Finally, TINCR upregulation slowed xenograft tumour growth in nude mice and significantly increased survival compared with control mice. Conclusion The results of this study suggest that TINCR inhibits the proliferation and invasion of LSCC by regulating the miR-210/BTG2 pathway, participates in cell cycle regulation, and may become a target for the treatment of LSCC.

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Section: Discussionsupporting

confidence: 87%

TINCR inhibits the proliferation and invasion of laryngeal squamous cell carcinoma by regulating miR-210/BTG2

Pang

Han

et al. 2021

BMC Cancer

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“…Further investigation revealed that microRNA-7 was negatively mediated by TINCR in bladder cancer cells. Silencing of TINCR expression significantly increased miR-7 expression and reduced bladder cancer cell proliferation, migration, and invasion, while knockdown of miR-7 expression reversed the inhibitory effects of TINCR downregulation on bladder cancer cells [ 37 ]. Increasing number of research on TINCR lncRNA in diverse cancer from 2019 declares its impressive role.…”

Section: Resultsmentioning

confidence: 99%

Identification of novel key regulatory lncRNAs in gastric adenocarcinoma

Razavi

Katanforosh

2022

BMC Genomics

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Background Stomach adenocarcinoma (STAD) is one of the most common and deadly cancers worldwide. Recent evidence has demonstrated that dysregulation of long noncoding RNAs (lncRNA) is associated with different hallmarks of cancer. lncRNAs also were suggested as novel promising biomarkers for cancer diagnosis and prognosis. Despite these previous investigations, the expression pattern, diagnostic role, and hallmark association of lncRNAs in STAD remain unclear. Results In this study, The STAD lncRNA-mRNA network was constructed based on RNAs that differentially expressed among tumor and normal samples and had a strong expression correlation with others. The high degree nodes of the network were associated with overall survival. In addition, we found that the hubs’ regulatory roles have previously been confirmed in different types of cancers by literature. For example, the HCG22 hub inhibited cell proliferation and invasion and induced apoptosis in oral squamous cell carcinoma (OSCC) cells. The levels of PCNA, Vimentin, and Bcl2 were decreased and E-cadherin and Bax expression was elevated in OSCC cells after HCG22 overexpression. Additionally, HCG22 overexpression inhibited the Akt, mTOR, and Wnt/β-catenin pathways. Then lncRNAs were mapped to their related GO terms and cancer hallmarks. Based on these mappings, we predict the hallmarks that might be associated with each lncRNA. Finally, the literature review confirmed our prediction. Among the 20 lncRNAs of the STAD network, 11 lncRNAs (LINC02560, SOX21-AS1, C5orf66-AS1, HCG22, PGM5-AS1, NALT1, ENSG00000241224.2, TINCR, MIR205HG, HNF4A-AS1, ENSG00000262756) demonstrated expression correlation with overall survival (OS). Based on expression analysis, survival analysis, hallmark associations, and literature review, LINC02560, SOX21-AS1, C5orf66-AS1, HCG22, PGM5-AS1, NALT1, ENSG00000241224.2, TINCR, MIR205HG, HNF4A-AS1 plays a regulatory role in STAD. For example, our prediction of association between C5orf66-AS1 expression dysregulation and “sustaining proliferative signal” and “Activating invasion and metastasis” has been confirmed in STAD, OSCC and cervical cancer. Finally, we developed a lncRNA signature with SOX21-AS1 and LINC02560, which classified patients into high and low-risk subgroups with significantly different survival outcomes. The mortality rate of the high-risk patients was significantly higher compared to the low-risk patients (28/1% vs 60.13). Conclusion These findings help in designing more precise and detailed experimental studies to find STAD biomarkers and therapeutic targets.

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“…Zhao et al demonstrated that ZEB1‑AS1 also induces migration and metastasis via AUF1-mediated translation activation of the ZEB1 mRNA mechanism [ 114 ]. Silencing of TINCR expression significantly reduces BCa cell proliferation, migration, and invasion by regulating miR-7 and mTOR expression [ 115 ]. HOTAIR promotes the proliferation, migration, and invasion of BCa cells by regulating CCNJ and inhibiting miRNA-205 [ 116 ].…”

Section: Invasion Migration and Metastasismentioning

confidence: 99%

“…The results of prognosis analysis revealed that high expression of CASC9 [ 102 , 148 ], SNHG3 [ 137 ], and SOX2OT [ 185 ], and low expression of LBCS [ 186 ] predict poor DFS. Elsewhere, high expression of CASC2a [ 190 ] increased the 5-year RFS rate, and high expression of 8 lncRNAs predicted a low RFS rate [ 21 , 23 , 37 , 38 , 115 , 146 , 156 , 164 , 188 , 193 ]. In addition, lower expression of 7 lncRNAs predicted shorter OS [ 24 , 26 , 57 , 126 , 150 , 152 , 186 ].…”

Section: Lncrnas That Influence Patient Prognosismentioning

confidence: 99%

Biological functions and clinical significance of long noncoding RNAs in bladder cancer

et al. 2021

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Bladder cancer (BCa) is one of the 10 most common cancers with high morbidity and mortality worldwide. Long noncoding RNAs (lncRNAs), a large class of noncoding RNA transcripts, consist of more than 200 nucleotides and play a significant role in the regulation of molecular interactions and cellular pathways during the occurrence and development of various cancers. In recent years, with the rapid advancement of high-throughput gene sequencing technology, several differentially expressed lncRNAs have been discovered in BCa, and their functions have been proven to have an impact on BCa development, such as cell growth and proliferation, metastasis, epithelial-mesenchymal transition (EMT), angiogenesis, and drug-resistance. Furthermore, evidence suggests that lncRNAs are significantly associated with BCa patients’ clinicopathological characteristics, especially tumor grade, TNM stage, and clinical progression stage. In addition, lncRNAs have the potential to more accurately predict BCa patient prognosis, suggesting their potential as diagnostic and prognostic biomarkers for BCa patients in the future. In this review, we briefly summarize and discuss recent research progress on BCa-associated lncRNAs, while focusing on their biological functions and mechanisms, clinical significance, and targeted therapy in BCa oncogenesis and malignant progression.

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lncRNA TINCR facilities bladder cancer progression via regulating miR‑7 and mTOR

Cited by 12 publications

References 30 publications

TINCR inhibits the proliferation and invasion of laryngeal squamous cell carcinoma by regulating miR-210/BTG2

TINCR inhibits the proliferation and invasion of laryngeal squamous cell carcinoma by regulating miR-210/BTG2

Identification of novel key regulatory lncRNAs in gastric adenocarcinoma

Biological functions and clinical significance of long noncoding RNAs in bladder cancer

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