LncRNA THUMPD3-AS1 enhances the proliferation and inflammatory response of chondrocytes in osteoarthritis

Wang, Yingjie; Tian, Li; Yang, Qi; Feng, Bin; Xiang, Yongbo; Lv, Zehui

doi:10.1016/j.intimp.2021.108138

Cited by 19 publications

(9 citation statements)

References 53 publications

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“…Accumulating evidence supports the important role of host-encoded lncRNAs in regulating immune response [ 15–17 ]. Some of the recent examples include: lncRNA OASL-IT1 and interferon loop system that regulates antiviral defense against Zika Virus [ 18 ]; lncRNA NLRP3 promotes NLRP3-triggered inflammatory response in early acute lung injury by binding to miR-138-5p [ 19 ]; mRNA-lncRNA crosstalk is involved in immune regulation of carp macrophages [ 20 ]; lncRNA THUMPD3-AS1 is downregulated in osteoarthritis and when overexpressed, it promotes cell proliferation, reduces apoptosis and facilitates inflammatory response [ 19 ]. Besides, host-encoded lncRNAs have also been found to regulate viral infection.…”

Section: Introductionmentioning

confidence: 99%

Differential expression of long non-coding RNAs under Peste des petits ruminants virus (PPRV) infection in goats

et al. 2022

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Peste des petits ruminants (PPR) characterized by fever, sore mouth, conjunctivitis, gastroenteritis, and pneumonia, is an acute, highly contagious viral disease of sheep and goats. The role of long non-coding RNAs (lncRNAs) in PPRV infection has not been explored to date. In this study, the transcriptome profiles of virulent Peste des petits ruminants virus (PPRV) infected goat tissues – lung and spleen were analyzed to identify the role of lncRNAs in PPRV infection. A total of 13,928 lncRNA transcripts were identified, out of which 170 were known lncRNAs. Intergenic lncRNAs (7625) formed the major chunk of the novel lncRNA transcripts. Differential expression analysis revealed that 15 lncRNAs (11 downregulated and 4 upregulated) in the PPRV infected spleen samples and 16 lncRNAs (13 downregulated and 3 upregulated) in PPRV infected lung samples were differentially expressed as compared to control. The differentially expressed lncRNAs (DElncRNAs) possibly regulate various immunological processes related to natural killer cell activation, antigen processing and presentation, and B cell activity, by regulating the expression of mRNAs through the cis- or trans-regulatory mechanism. Functional enrichment analysis of differentially expressed mRNAs (DEmRNAs) revealed enrichment of immune pathways and biological processes in concordance with the pathways in which correlated lncRNA-neighboring genes were enriched. The results suggest that a coordinated immune response is raised in both lung and spleen tissues of the goat through mRNA-lncRNA crosstalk.

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Section: Introductionmentioning

confidence: 99%

Differential expression of long non-coding RNAs under Peste des petits ruminants virus (PPRV) infection in goats

et al. 2022

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“…Macrophages play an important role in a wide variety of physiologic and pathologic processes ( Cocks et al, 2021 ). Previous studies have found that osteogenic and angiogenic effects may be indirectly regulated through the immune response, in which macrophage polarization and cytokine secretion highly affect the fate of bone implants ( Chen et al, 2020 ; Wang Y. et al, 2021 ; Li et al, 2021 ). Biomaterial implantation immediately stimulates the innate immune system and triggers a response that usually occurs in sequential stages, ranging from inflammation to tissue proliferation and maturation ( Thorson et al, 2019 ; Zhao et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensionally Printed Ti2448 With Low Stiffness Enhanced Angiogenesis and Osteogenesis by Regulating Macrophage Polarization via Piezo1/YAP Signaling Axis

Tang

Wei

Tian

et al. 2021

Front. Cell Dev. Biol.

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Previous studies have found that the novel low-elastic-modulus Ti2448 alloy can significantly reduce stress shielding and contribute to better bone repair than the conventional Ti6Al4V alloy. In this study, the promotion of osteogenesis and angiogenesis by three-dimensionally printed Ti2448 were also observed in vivo. However, these were not significant in a series of in vitro tests. The stiffness of materials has been reported to greatly affect the response of macrophages, and the immunological regulation mediated by macrophages directly determines the fate of bone implants. Therefore, we designed more experiments to explore the role of three-dimensionally printed Ti2448 in macrophage activation and related osteogenesis and angiogenesis. As expected, we found a significant increase in the number of M2 macrophages around Ti2448 implants, as well as better osteogenesis and angiogenesis in vivo. In vitro studies also showed that macrophages pre-treated with Ti2448 alloy significantly promoted angiogenesis and osteogenic differentiation through increased PDGF-BB and BMP-2 secretion, and the polarization of M2 macrophages was enhanced. We deduced that Ti2448 promotes angiogenesis and osteogenesis through Piezo1/YAP signaling axis-mediated macrophage polarization and related cytokine secretion. This research might provide insight into the biological properties of Ti2448 and provide a powerful theoretical supplement for the future application of three-dimensionally printed Ti2448 implants in orthopaedic surgery.

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“…confirmed that Thumpd3-as1 can regulate the self-renewal of non–small cell lung cancer cells ( Hu et al., 2019 ); the findings of Wang et al. confirmed that Thumpd3-as1 overexpression enhanced the inflammatory response of chondrocytes in osteoarthritis ( Wang et al., 2021 ); the study of Xia et al. confirmed that Thumpd3-as1 is a lncRNA related to the immune microenvironment of colon adenocarcinoma ( Xia et al., 2021 ).…”

Section: Discussionmentioning

confidence: 91%

Identification and Validation of Immune-Related Biomarker Gene and Construction of ceRNA Networks in Septic Cardiomyopathy

et al. 2022

Front. Cell. Infect. Microbiol.

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Septic cardiomyopathy (SCM) is a cardiac dysfunction caused by severe sepsis, which greatly increases the risk of heart failure and death, and its molecular mechanism is unclear. The immune response has been reported to be an important process in septic cardiomyopathy and is present in the cardiac tissue of patients with sepsis, suggesting that the immune response may be an underlying mechanism of myocardial injury in SCM. Therefore, we explored the role of immune-related genes (IRGs) in SCM and aimed to identify pivotal immune-related targets with the aim of identifying key immune-related targets in SCM and potential therapeutic mechanisms involved in the pathological process of SCM. To explore the regulatory mechanisms of immune responses in SCM, we identified differentially expressed genes (DEGs) shared in the SCM datasets GSE179554 and GSE40180 by bioinformatics analysis and then obtained hub genes from the DEGs. Then, we obtained the immune-related hub genes (IRHGs) by intersecting the hub genes with IRGs and performed quantitative reverse transcription polymerase chain reaction to confirm the abnormal expression of IRHGs. Finally, we further constructed an immune-related lncRNA–miRNA–IRHG ceRNA regulatory network. In this study, we identified an IRHG that may be involved in the pathogenesis of SCM, which helps us to further elucidate the role of immune response in SCM and gain insights into the molecular mechanisms and potential therapeutic targets of SCM.

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LncRNA THUMPD3-AS1 enhances the proliferation and inflammatory response of chondrocytes in osteoarthritis

Cited by 19 publications

References 53 publications

Differential expression of long non-coding RNAs under Peste des petits ruminants virus (PPRV) infection in goats

Differential expression of long non-coding RNAs under Peste des petits ruminants virus (PPRV) infection in goats

Three-Dimensionally Printed Ti2448 With Low Stiffness Enhanced Angiogenesis and Osteogenesis by Regulating Macrophage Polarization via Piezo1/YAP Signaling Axis

Identification and Validation of Immune-Related Biomarker Gene and Construction of ceRNA Networks in Septic Cardiomyopathy

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