LncRNA SNHG4 regulates miR-10a/PTEN to inhibit the proliferation of acute myeloid leukemia cells

Yuan, Zhongtao; Wang, Wei

doi:10.1080/16078454.2020.1754636

Cited by 21 publications

(18 citation statements)

References 17 publications

(19 reference statements)

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“…Long noncoding RNAs (lncRNAs), which are more than 200 nucleotides in length, were once considered unrelated transcriptional waste with no biological function [ 3 ]; however, the competing endogenous RNA (ceRNA) hypothesis indicates that mRNA, transcriptional pseudogenes and lncRNAs interact with each other by competing for microRNA (miRNA) binding [ 4 ]. LncRNAs, including ceRNAs, have been found to be widely involved in the biological processes of many diseases by regulating the amount and function of miRNAs, thus affecting downstream signalling pathways [ 5 , 6 ]. In addition, many lncRNAs have been shown to be differentially expressed (DE) in HPH rats compared with healthy rats [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Construction and analysis of the abnormal lncRNA–miRNA–mRNA network in hypoxic pulmonary hypertension

et al. 2021

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The incidence of hypoxic pulmonary hypertension (HPH) is increasing. Accumulating evidence suggests that long non-coding RNAs (lncRNAs) play an important role in HPH, but the functions and mechanism have yet to be fully elucidated. In this study, we established an HPH rat model with 8 h of hypoxia exposure (10% O2) per day for 21 days. High-throughput sequencing identified 60 differently expressed (DE) lncRNAs, 20 DE miRNAs and 695 DE mRNAs in rat lung tissue. qRT-PCR verified the accuracy of the results. Immune response, inflammatory response, leukocyte migration, cell cycle, cellular response to interleukin-1, IL-17 signalling pathway, cytokine-cytokine receptor interaction and Toll-like receptor signalling pathway were significantly enriched in DE mRNAs. According to the theory of competing endogenous RNA (ceRNA) networks, lncRNA-miRNA-mRNA network was constructed by Cytoscape software, including 7 lncRNAs, 16 miRNAs and 144 mRNAs. The results suggested that seven DE lncRNAs (Ly6l, AABR07038849.2, AABR07069008.2, AABR07064873.1, AABR07001382.1, AABR07068161.1 and AABR07060341.2) could serve as molecular sponges of the corresponding miRNAs and play a major role in HPH.

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Section: Introductionmentioning

confidence: 99%

Construction and analysis of the abnormal lncRNA–miRNA–mRNA network in hypoxic pulmonary hypertension

et al. 2021

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“…Moreover, it has been revealed that PTEN is functionally lost in human leukemia through deletion, mutation, transcriptional silencing, and protein inactivation 35 . Two recent studies reported that the expression of PTEN in AML cells could be regulated by long noncoding RNAs via sponging targeted miRNAs 36,37 . Here, we found that PTEN is a downstream target of miR‐516b.…”

Section: Discussionmentioning

confidence: 59%

Circular RNA circ_0040823 inhibits the proliferation of acute myeloid leukemia cells and induces apoptosis by regulating miR‐516b/PTEN

Wang

Yang

Jen

et al. 2022

The Journal of Gene Medicine

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Objective Endogenous circular RNAs (circRNAs) and microRNAs (miRNAs) have been shown to regulate the pathogenesis of acute myeloid leukemia (AML). The current study aimed to identify the role of circRNA 0040823 (circ_0040823) in AML. Methods Microarray datasets were analyzed to identify differentially expressed circRNAs in AML patients. Peripheral blood samples were obtained from healthy volunteers and AML patients for the measurement of circ_0040823 and miR‐516b levels. The overexpression or knockdown of a target gene in AML cells was achieved by the transfection with lentiviral vectors or small interfering RNAs. BALB/c nude mice were inoculated with AML cells and monitored for tumor growth. Dual‐luciferase reporter assay, RNA immunoprecipitation, and RNA pull‐down assay were used to determine the binding relationship between circRNA and miRNA. Results circ_0040823 was significantly downregulated in AML patients and leukemia cells. Overexpression of circ_0040823 inhibited AML cell proliferation, and induced apoptosis and cell cycle arrest. Upregulation of circ_0040823 also repressed the growth of xenograft tumors in vivo. circ_0040823 acted as a miR‐516b sponge and regulated key cellular events in leukemia cells via downregulating miR‐516b. Moreover, tumor suppressor phosphatase and tensin homolog (PTEN) was a downstream target of miR‐516b. The inhibition of miR‐516b impaired the proliferation capacity of leukemia cells and induced apoptosis, while PTEN deficiency attenuated these effects. Conclusion This study showed that circ_0040823 inhibited proliferation and induced apoptosis of AML cells by sponging miR‐516b, thereby diminishing the regulatory effect of miR‐516b on PTEN. These findings identified circ_0040823/miR‐516b/PTEN as a new therapeutic target for AML.

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“…SNHG4 has been reported to be abnormally expressed in many diseases. It is downregulated in patients with neonatal pneumonia (NP) [ 30 ], diabetic retinopathy (DR) [ 19 ], acute cerebral infarction (ACI) [ 20 ], and acute myeloid leukaemia (AML) [ 21 ] and is upregulated in gastric cancer (GC) [ 31 ], renal cell carcinoma (RCC) [ 32 ], glioblastoma (GBM) [ 33 ], neuroblastoma [ 34 ], colorectal cancer (CRC) [ 35 ], etc. The aberrant expression of SNHG4 has been proven to be closely related to the genesis, occurrence and progression of various diseases.…”

Section: Lncrna Snhg4 a Member Of The Lncrna Snhg Familymentioning

confidence: 99%

“…In ACI [ 20 ], NP [ 30 ], DR [ 19 ], AML [ 21 ], neuroblastoma [ 33 ], CRC [ 35 ], cervical cancer (CC) [ 80 ], non-small-cell lung cancer (NSCLC) [ 28 ], and endometriosis [ 81 ], researchers explored the expression of SNHG4 in patients with these diseases. However, they did not seem to have studied the relationship between SNHG4 and the clinical features of these diseases, nor have they explored the relationship between SNHG4 and disease prognosis.…”

Section: Snhg4 In Human Studiesmentioning

confidence: 99%

“…In addition, SNHG4 can function as a ceRNA, sponge miR-138 to upregulate c-Met [ 33 ], sponge miR-204-5p to upregulate RUNX2 [ 32 ], and then promote glioblastoma cell and RCC cell proliferation. Although SNHG4 plays a proliferation-promoting role in most diseases, it is downregulated in AML and inhibits proliferation by regulating the miR-10a/PTEN axis [ 21 ]. SNHG4 sponges miR-10a to upregulate PTEN, which acts as a tumour suppressor and can inhibit cancer cell proliferation.…”

Section: Snhg4 In Cell Lines Studiesmentioning

confidence: 99%

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Long noncoding RNA SNHG4: a novel target in human diseases

Chu

Zheng

et al. 2021

Cancer Cell Int

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Recently, long noncoding RNAs (lncRNAs) have attracted great attention from researchers. LncRNAs are non-protein-coding RNAs of more than 200 nucleotides in length. Multiple studies have been published on the relationship between lncRNA expression and the progression of human diseases. LncRNA small nucleolar RNA host gene 4 (SNHG4), a member of the lncRNA SNHG family, is abnormally expressed in a variety of human diseases, including gastric cancer, renal cell carcinoma, glioblastoma, neuroblastoma, prostate cancer, colorectal cancer, osteosarcoma, cervical cancer, liver cancer, lung cancer, non-small-cell lung cancer, neonatal pneumonia, diabetic retinopathy, neuropathic pain, acute cerebral infarction, acute myeloid leukaemia, and endometriosis. In this paper, the structure of SNHG4 is first introduced, and then studies in humans, animal models and cells are summarized to highlight the expression and function of SNHG4 in the above diseases. In addition, the specific mechanism of SNHG4 as a competing endogenous RNA (ceRNA) is discussed. The findings indicate that SNHG4 can be used as a biomarker for disease prognosis evaluation and as a potential target for disease diagnosis and treatment.

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LncRNA SNHG4 regulates miR-10a/PTEN to inhibit the proliferation of acute myeloid leukemia cells

Cited by 21 publications

References 17 publications

Construction and analysis of the abnormal lncRNA–miRNA–mRNA network in hypoxic pulmonary hypertension

Construction and analysis of the abnormal lncRNA–miRNA–mRNA network in hypoxic pulmonary hypertension

Circular RNA circ_0040823 inhibits the proliferation of acute myeloid leukemia cells and induces apoptosis by regulating miR‐516b/PTEN

Long noncoding RNA SNHG4: a novel target in human diseases

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