lncRNA SNHG3 promotes breast cancer�progression by acting as a miR‑326 sponge

Abstract

Accumulating evidence suggests that long noncoding RNA (lncRNA) small nucleolar RNA host gene 3 (SHNG3) plays crucial roles in the initiation and progression of various types of malignant cancers. Yet, the role played by SNHG3 in breast cancer as well as the associated mechanisms remain largely unclear. The expression of SNHG3 was detected in breast cancer tissues and cell lines by reverse-transcription quantitative PCR (RT-qPCR). Cell proliferation, colony formation, cell cycle distribution, migration and invasion abilities were detected by Cell Counting Kit-8, colony formation assay, flow cytometry, wound-healing and Matrigel invasion assays, respectively. The regulatory relationships between SNHG3 and miR-326 were explored by luciferase reporter assay. A nude mouse model was established to investigate the effect of SNHG3 in vivo. The results showed an upregulation of SNHG3 in breast cancer tissues and cell lines. Loss-of-function assays revealed significant suppression of breast cancer behaviors such as: Abilities to proliferate, form colonies, migrate and invade in vitro coupled with a delayed growth of tumors in vivo when SNHG3 was knocked down. Mechanically, it was shown that SNHG3 served as a competing endogenous RNA (ceRNA) of miR-326 that in turn is a tumor suppressor in this cancer. The correlation between the expression of SNHG3 and miR-326 was found to be strongly negative in these samples. Additionally, we found that inhibition of SNHG3 caused a partially reversal in the inhibition exerted by miR-326 on the ability of these cells to proliferate, form colonies, migrate and invade. Collectively, these findings suggest the functioning of SNHG3 as a ceRNA to enhance the ability of breast cancer cells to proliferate and metastasize to putatively serve as a new target to explore therapeutic intervention of this malignancy.

Long noncoding RNA SNHG10 promotes colorectal cancer cells malignant progression by targeting miR-3690

“…The mechanism studies of LncRNAs showed that lncRNAs could regulate genes expression as miRNA sponges [ 21 , 22 ]. Therefore, the potential target miRNAs of SNHG10 were predicted by bioinformatics analysis.…”

Development and Validation of a Prognostic Signature Based on Autophagy-related Long Non-coding RNA Analysis in Hepatocellular Carcinoma

“…Additionally, the role of SNHG3 in caner has been gradually uncovered [30]. Increasing studies indicated that SNHG3 affects the tumor development and progression by regulating autophagy-related microRNAs, genes, or corresponding pathways [31][32][33][34]. MIR210HG [35][36][37][38], and ZEB1-AS1 [39,40] have also been found to affect tumorigenesis, progression, and tumor metastasis, resulting in a poor prognosis for patients with cancer.…”

Small nucleolar RNA host gene 3 functions as a novel biomarker in liver cancer and other tumour progression

“…SNHG3 exhibits characteristic oncogenic properties in multiple cancers. Studies have indicated that SNHG3 is significantly upregulated in hepatocellular carcinoma (HCC)[ 25 , 26 ] and other cancers, such as cervical cancer (CC)[ 27 , 28 ], and breast cancer (BC)[ 29 - 34 ]. Clear cell renal cell carcinoma (ccRCC)[ 35 ], colorectal cancer (CRC)[ 36 - 38 ], glioma[ 39 , 40 ], HCC[ 25 , 41 , 42 ], non-small cell lung cancer (NSCLC)[ 43 - 47 ], ovarian cancer (OC)[ 48 - 51 ], and papillary thyroid carcinoma (PTC)[ 52 ].…”

“…HCC growth was inhibited in vivo due to the knockdown of the SNHG3 gene[ 25 ]. Similarly, SNHG3 gene knockdown inhibited the growth of other tumors, such as BC[ 34 ], CC[ 27 ], ccRCC[ 35 ], CRC[ 36 , 37 ], GC[ 77 ], glioma[ 40 ], laryngeal carcinoma[ 80 ], NSCLC[ 45 , 46 ], OC[ 49 ], PTC[ 52 ], prostate cancer[ 85 ]. Overall, SNHG3 promoted the progression of the abovementioned tumors.…”