LncRNA SNHG14 promotes non-small cell lung cancer progression by sponging miR-382-5p

Abstract

Long non-coding RNAs (lncRNAs) are involved in development of non-small cell lung cancer (NSCLC) by interacting with microRNAs (miRNAs) and/or mRNAs. However, the function of most lncRNAs in NSCLC remains unclear. Reverse transcription-quantitative PCR (RT-qPCR) and western blot were applied for detection of mRNA/miRNA and protein level. Interaction between LncRNA small nucleolar RNA host gene 14 (SNHG14) and miR-382-5p was validated by dual luciferase reporter assay. Cell proliferation and apoptosis was detected using Cell Counting Kit 8 (CCK8) and Flow cytometry, while cell migration and invasion was detected by scratch assay and Transwell assay. SNHG14 was upregulated in NSCLC tissues and cell lines. SNHG14 silencing reduced proliferation, migration and invasion, and induced apoptosis in A549 cells. SNHG14 was negatively correlated with miR-382-5p in NSCLC tissues, SNHG14 and miR-382-5p negatively regulated each other in A549 cells. SNHG14 promoted expression of miR-382-5p target genes, including LIM-only protein 3 (LMO3) and SET domain containing lysine methyltransferase 8 (SETD8), in A549 cells. miR-382-5p inhibition reversed SNHG14 knockdown-induced increase in apoptosis and decrease in proliferation, migration and invasion. SNHG14 was correlated with SETD8 and LMO3 in NSCLC tissues. Collectively, SNHG14 promoted NSCLC proliferation, migration and invasion, while inhibited apoptosis by sponging miR-382-5p in A549 cells.

Overexpression of LncRNA SNHG14 as a biomarker of clinicopathological and prognosis value in human cancers: A meta-analysis and bioinformatics analysis

“…One hundred thirteen studies required further screening based on their title and abstract. Subsequently, 47 studies were eligible to read the full text, and eventually, 21 cohort studies ( Zhang Y. Y. et al, 2019 ; Zhang Z. et al, 2019 ; Deng et al, 2019 ; Ji et al, 2019 ; Li et al, 2019 ; Pei et al, 2019 ; Zhao et al, 2019 ; Zhao and Huang, 2019 ; Zhang H. et al, 2020 ; Zhang K. et al, 2020 ; Chen et al, 2020 ; Zhang W. et al, 2020 ; Luo et al, 2020 ; Sun et al, 2020 ; Wang and Wen, 2020 ; Xu et al, 2020 ; Zhou et al, 2020 ; Wang et al, 2021a ; Wang et al, 2021b ; Feng et al, 2021 ; Liao et al, 2021 ) were included in the meta-analysis ( Figure 1 ).…”

“…The expression of SNHG14 was detected using qPCR within the included studies. In addition, 16 studies Zhang Z. et al, 2019;Ji et al, 2019;Li et al, 2019;Pei et al, 2019;Zhao and Huang, 2019;Zhang H. et al, 2020;Zhang K. et al, 2020;Chen et al, 2020;Zhang W. et al, 2020;Luo et al, 2020;Sun et al, 2020;Wang and Wen, 2020;Feng et al, 2021;Liao et al, 2021) reported on OS, and 17 Zhang Z. et al, 2019; Frontiers in Genetics frontiersin.org Frontiers in Genetics frontiersin.org Deng et al, 2019;Ji et al, 2019;Zhang H. et al, 2020;Zhang K. et al, 2020;Chen et al, 2020;Zhang W. et al, 2020;Luo et al, 2020;Sun et al, 2020;Wang and Wen, 2020;Xu et al, 2020;Zhou et al, 2020;Wang et al, 2021a;Wang et al, 2021b;Feng et al, 2021;Liao et al, 2021) on clinical outcomes. The NOS scores of included studies were as follows: two studies (Zhang Z. et al, 2019;Zhang W. et al, 2020) with a NOS score of 8, five studies (Ji et al, 2019;Zhang K. et al, 2020;Wang et al, 2021a;Wang et al, 2021b;Feng et al, 2021) with a NOS score of 7, eight studies Deng et al, 2019;Pei et al, 2019;Chen et al, 2020;…”

“…Relationship between SNHG14 gene expression and infiltrating levels of immune cells in LGG, PAAD, MESO, SKCM, LIHC. Feng (Feng et al, 2021), Li (Li et al, 2019) Non-small cell lung cancer Upregulate Cell proliferation, apoptosis, invasion migration, metastasis miR-382-5p, miR-340 Zhou (Zhou et al, 2020) Zhao (Zhao and Huang, 2019), Zhao Frontiers in Genetics frontiersin.org…”

Clinicopathological significance and prognosis of long noncoding RNA SNHG14 expression in human cancers: A Meta-Analysis and bioinformatics analysis

“…After removing duplication, we further screened 113 studies based on their title, abstract. 51 studies need to read the full text, and eventually 21 [19,[26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45] were included in this meta-analysis (Figure 1). This study included 1,080 patients, all from China.…”

“…The expression of SNHG14 was detected by qPCR in 21 studies. Among the included studies, 16 [19, 27, 28, 31-33, 35-39, 41-45]reported on OS and 17 [19,[26][27][28][29][30][31][32][33][34][35][36][37][38][39][40]43] on clinical outcomes. The NOS scores for all included studies were ≥7, which indicates that the methodological quality of included studies was high.…”