lncRNA-SNHG14 Promotes Atherosclerosis by Regulating RORα Expression through Sponge miR-19a-3p

Abstract

Coronary heart disease (CHD) is the most common cardiovascular disease with high prevalence, disability, and mortality. The balance between proliferation and apoptosis of vascular smooth muscle cells (VSMCs) plays a key role in the initiation of atherosclerosis. In this study, we found a significant decrease in the expression of lncRNA-SNHG14 in atherosclerotic plaque tissues of ApoE-/- mice. Overexpression of lncRNA-SNHG14 can inhibit VSMC proliferation while promoting apoptosis. There is a potential reciprocal regulatory relationship between lncRNASNHG14 and miR-19a-3p, which inhibit each other’s expression in vascular smooth muscle cells. In addition, the luciferase reporter gene analysis results showed that there was a direct interaction between miR-19a-3p and the 3′UTR of RORα. The results of qRT-PCR showed that the level of RORα mRNA was significantly increased in the aortas treated with miR-19a-3p and SNHG14 compared with that treated with miR-19a-3p alone. In conclusion, we demonstrated that lncRNA-SNHG14 regulates the apoptosis/proliferation balance of VSMCs in atherosclerosis.

Dysregulation Serum miR-19a-3p is a Diagnostic Biomarker for Asymptomatic Carotid Artery Stenosis and a Promising Predictor of Cerebral Ischemia Events

“…27 Notably, miR-19a-3p is upregulated in atherosclerosis. 12 In addition, it has been reported that miR-19a-3p has a dual role in ischemic diseases. It can improve myocardial ischemia by regulating angiogenesis and myocardial fibrosis, 28 but also can promote ischemic stroke by interfering with glucose metabolism and promoting neuronal apoptosis.…”

“… 11 miR-19a-3p has been reported to be involved in atherosclerosis, and atherosclerosis is considered to be a common cause of CAS. 12 More importantly, in transient ischemic attack (TIA), a complication of CAS, glycine improves TIA by regulating miR-19a-3p to inhibit glucose metabolism, inflammation, and apoptosis. 13 …”

“…11 miR-19a-3p has been reported to be involved in atherosclerosis, and atherosclerosis is considered to be a common cause of CAS. 12 More importantly, in transient ischemic attack (TIA), a complication of CAS, glycine improves TIA by regulating miR-19a-3p to inhibit glucose metabolism, inflammation, and apoptosis. 13 Although the function of miR-19a-3p in CAS-related diseases has been described in detail, the clinical potential of miR-19a-3p in asymptomatic CAS remains unknown.…”

Long noncoding RNA SNHG10 promotes colorectal cancer cells malignant progression by targeting miR-3690

“…The mechanism studies of LncRNAs showed that lncRNAs could regulate genes expression as miRNA sponges [ 21 , 22 ]. Therefore, the potential target miRNAs of SNHG10 were predicted by bioinformatics analysis.…”

Long Noncoding RNA-Mediated Competing Endogenous RNA Networks in Atherosclerosis: Regulate VSMC proliferation, migration, and apoptosis

“…Sun et al con rmed that SNHG12 targets miR-199a-5p to regulate HIF-1α, thereby stimulating its proliferation and migration, and promoting the occurrence and development of AS[46]. Zhu, et al found that lncRNA SNHG14 can regulate miR-19a-3p/RORα axis through ceRNA mechanisms to inhibit the proliferation and promote apoptosis of VSMCs in AS, thus contributing to the development of AS[47].The peroxisome proliferator-activated receptors (PPARs) are ligand-activated nuclear TFs. PPARs are members of type II nuclear receptor superfamily and have multiple functions, such as inhibiting cell cycle progression from G1 to the S phase.…”