LncRNA RPL34-AS1 suppresses the proliferation, migration and invasion of esophageal squamous cell carcinoma via targeting miR-575/ACAA2 axis

Zhang, Hu; Pan, Enchun; Zhang, Ying; Zhao, Chunjiang; Liu, Qiwei; Pu, Yuepu; Ye, Lihong

doi:10.1186/s12885-022-10104-6

Cited by 8 publications

(3 citation statements)

References 45 publications

(45 reference statements)

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“…One study retrospectively analyzed the differences in immune cell infiltration and functional phenotypes in the tumor cells of 72 EAC patients, and found that the cytokine staining had a mixture functions of pro-inflammatory and anti-inflammatory properties in EAC tumors tissue and found that memory cytotoxic T cells were rich in memory cytotoxic T cells (26). This result will greatly promote the study of the EAC tumor immune microenvironment and immune cell reaction mechanism (31).…”

Section: Discussionmentioning

confidence: 99%

Model construction and risk analysis of the lncRNA genes associated with the prognosis of esophageal adenocarcinoma with immune infiltration

Yang¹,

Ma²,

Wang³

et al. 2023

J Gastrointest Oncol

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Background: Our study analyzed the immune infiltration of esophageal adenocarcinoma (EAC) tumor cells and identified long non-coding ribonucleic acid (lncRNA) genes to construct a prognostic model of EAC to evaluate the survival prognosis of patients and explore potential therapeutic targets. Methods:The data of 89 patients with EAC, including 11 normal tissue samples and 78 EAC of tumor tissue samples, were downloaded from The Cancer Genome Atlas public database. Perl script and R software were used to run the code, conduct the statistical analysis, calculate the risk coefficients of the patients, and conduct the Cox regression analysis, immune-related lncRNA survival analysis, risk analysis, principal component analysis (PCA), and receiver operating characteristic (ROC) curve analysis.Results: We screened and identified 19 prognostic biomarkers, including LINC01612, AC008443.2, and LINC02582, allocated the patients into high-and low-risk groups, and found significant differences in the prognosis between the high-and low-risk groups using the Kaplan-Meier survival analysis (P<0.001).A ROC curve was used to evaluate the feasibility of the prognostic model for EAC, and we found that the model had high predictability (area under the curve =0.964). A PCA analysis was performed of the complex transcriptome sequencing data and other cubes to transform the data into a 3-dimensional space constructed by feature vectors.Conclusions: Our study effectively screened and identified the lncRNA genes related to the immune infiltration of EAC and successfully constructed a prognostic model. In total, 19 potential diagnostic and therapeutic target genes, including LINC01612, AC008443.2, and LINC02582, were identified that have certain significance in guiding the clinical treatment of EAC patients.

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Section: Discussionmentioning

confidence: 99%

Model construction and risk analysis of the lncRNA genes associated with the prognosis of esophageal adenocarcinoma with immune infiltration

Yang¹,

Ma²,

Wang³

et al. 2023

J Gastrointest Oncol

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“…WD repeat and FYVE domain containing 3-AS2, via miR-2355-5p/suppressor of cytokine signaling 2, impeded the JAK2/STAT5 pathway, thereby suppressing proliferation, invasion and EMT in EC9706 and TE-1 cells, with implications for TNM staging, lymph node metastasis and prognosis ( 130 ). In addition, ribosomal protein L34 (RPL34)-AS1, through the miR-575/acetyl-CoA acyltransferase 2 (ACAA2) axis, demonstrated inhibitory effects on proliferation, clone formation, invasion and migration of EC109 cells, with an influence on OS ( 131 ).…”

Section: Lncrnas Downregulated In Esccmentioning

confidence: 99%

Roles of long non‑coding RNAs in esophageal cell squamous carcinoma (Review)

Yan,

Wong,

Gong

et al. 2024

Int J Mol Med

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“…For instance, lncRNA ATB orchestrates the miR-200b/Kindlin-2 axis to facilitate ESCC progression 11 . Similarly, lncRNA RPL34-AS1 regulates ESCC proliferation and migration through the miR-575/ACAA2 axis 12 . Furthermore, LINC00680 functions as an oncogene in ESCC development by upregulating CDK6 expression and can promote ESCC progression through miR-497 sponging 13 .…”

Section: Introductionmentioning

confidence: 99%

LncRNA SNHG1 acts as a ceRNA for miR-216a-3p to regulate TMBIM6 expression in esophageal squamous cell carcinoma

Kong,

Chi,

et al. 2024

J. Cancer

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Background: The long noncoding RNA small nucleolar RNA host gene 1 (SNHG1) has been demonstrated to play a crucial role in the progression of esophageal squamous cell carcinoma (ESCC). The current study aims to explore the deeper molecular mechanisms of SNHG1 in ESCC. Methods: Fifty patients with ESCC were enrolled to assess overall survival. Quantitative real-time PCR was performed to measure the levels of SNHG1, miR-216a-3p, and TMBIM6 in ESCC cells. Functional assessments of SNHG1 on ESCC cells were conducted using CCK-8 assay, flow cytometry, and Transwell assays. Western blot was conducted to detect the protein levels of TMBIM6 and proapoptotic proteins (Calpain and Caspase-12). The interaction among SNHG1, miR-216a-3p, and TMBIM6 was assessed with luciferase reporter assays. Results: Our study revealed that SNHG1 was notably increased in both clinical ESCC samples and cellular lines. Upregulation of SNHG1 in ESCC tissues was indicative of poor overall survival. Functionally, SNHG1 knockdown significantly inhibited the proliferation, migration, and invasion while promoting apoptosis in ESCC cells. Mechanistically, SNHG1 functioned as a competing endogenous RNA by sequestering miR-216a-3p to modulate TMBIM6 levels in ESCC cells. Notably, inhibiting miR-216a-3p or restoring TMBIM6 reversed the inhibitory effect induced by SNHG1 knockdown in ESCC cells. Conclusions: We demonstrate for the first time that SNHG1 may act as a competing endogenous RNA and promote ESCC progression through the miR-216a-3p/TMBIM6 axis. This highlights the potential of SNHG1 as a target for ESCC treatment.

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LncRNA RPL34-AS1 suppresses the proliferation, migration and invasion of esophageal squamous cell carcinoma via targeting miR-575/ACAA2 axis

Cited by 8 publications

References 45 publications

Model construction and risk analysis of the lncRNA genes associated with the prognosis of esophageal adenocarcinoma with immune infiltration

Model construction and risk analysis of the lncRNA genes associated with the prognosis of esophageal adenocarcinoma with immune infiltration

Roles of long non‑coding RNAs in esophageal cell squamous carcinoma (Review)

LncRNA SNHG1 acts as a ceRNA for miR-216a-3p to regulate TMBIM6 expression in esophageal squamous cell carcinoma

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