LncRNA RP11-138J23.1 Contributes to Gastric Cancer Progression by Interacting With RNA-Binding Protein HuR

Abstract

In spite of improvements in diagnostics and treatment of gastric cancer (GC), it remains the most common malignancy of human digestive system. It is now widely appreciated that long noncoding RNAs (lncRNAs) exert extensive regulatory effects on a spectrum of fundamental biological processes through diverse mechanisms. In this study, we explored the expression level and functional role of lncRNA RP11-138J23.1 in GC. Through bioinformatics analyses and in situ hybridization (ISH), we identified that RP11-138J23.1 was upregulated in GC tissue. Further study showed that RP11-138J23.1 knockdown significantly inhibited cell proliferation and metastatic ability. Whereas, RP11-138J23.1 overexpression could promote tumor cell growth and metastasis in vitro. Additionally, loss-of-function assays were used to confirm the role of RP11-138J23.1 in vivo. Mechanistically, RP11-138J23.1 exerted its oncogenic functions by binding to HuR protein and increasing stability of VAV3 mRNA. Overall, our study highlights the essential role of RP11-138J23.1 in GC, suggesting that RP11-138J23.1 might be a potent therapeutic target for patients with GC.

LncRNA MEG3 Reduces the Ratio of M2/M1 Macrophages Through the HuR/CCL5 Axis in Hepatocellular Carcinoma

“…For example, lncRNA RP11-138J23.1 enhances the metastatic and proliferative abilities of gastric cancer cells by increasing VAV3 mRNA stability via interaction with HuR. 52 The lncRNA ADORA2A-AS1 displays antitumor effects in HCC by binding to HuR and inhibiting the FSCN1/AKT axis. 35 Through bioinformatic analysis in this study, nine RBPs were identified with a high probability of binding to MEG3.…”

A standardised nomenclature for long non‐coding RNAs

“…These clone‐based identifiers used to be displayed on Ensembl gene reports for human genes that had no HGNC symbol, but have now been removed completely and are not searchable in the current version of the Ensembl website. We recently found the following examples in the literature: AF178030.2 47 which has the approved HGNC symbol TRPS1‐AS1 ; RP11‐138 J23.1, 48 which has the approved symbol NIHCOLE ; RP11‐276H19.1 49 has approved symbol GAS1RR ; and RP3‐326I13.1 50 which has the approved symbol PINCR . We urge researchers to check genenames.org ahead of publication to see if there is an approved symbol for an lncRNA gene.…”

“…following examples in the literature: AF178030.247 which has the approved HGNC symbol TRPS1-AS1; RP11-138 J23.1,48 which has the approved symbol NIH-COLE; RP11-276H19.149 has approved symbol GAS1RR; and RP3-326I13.150 which has the approved symbol PINCR. We urge researchers to check genenames.org ahead of publication to see if there is an approved symbol for an lncRNA gene.…”

GATA4-activated lncRNA MALAT1 promotes osteogenic differentiation through inhibiting NEDD4-mediated RUNX1 degradation

“…Previous reports indicated that lncRNAs regulated the stability of target mRNA and participated in the process of multiple disease by interacting with RNA-binding proteins (RBPs) [ 14 , 15 ]. For instance, lncRNA RP11-138J23.1 was reported to participated in the progression of gastric cancer through enhancing the stability of VAV3 mRNA by targeting HuR protein [ 16 ]. K-homology splicing regulatory protein (KHSRP), as a RBP, was demonstrated to be vital in the process of mRNA metabolism.…”