LncRNA PVT1 promotes malignant progression in squamous cell carcinoma of the head and neck

Yu, Changyun; Wang, Yunyun; Guo, Li; She, Li; Zhang, Diekuo; Chen, Xiyu; Zhang, Xin; Qin, Zhaobing; Cao, Hua; Liu, Yong

doi:10.7150/jca.26465

Cited by 25 publications

(23 citation statements)

References 47 publications

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“…Long noncoding RNAs (lncRNAs) play an important role in various biological and pathological processes frequently implicated in cancer, including proliferation, apoptosis, cell cycle progression, migration, and invasion [4]. Plasmocytoma variant translocation 1 (PVT1) is a long nonprotein-coding gene located at the 8q24 human chromosomal region and is commonly dysregulated in PCa [5,6].…”

Section: Introductionmentioning

confidence: 99%

Long Noncoding RNA from PVT1 Exon 9 Is Overexpressed in Prostate Cancer and Induces Malignant Transformation and Castration Resistance in Prostate Epithelial Cells

Pal

Huaman

Levine

et al. 2019

Genes

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Prostate cancer (PCa) is the most common non-cutaneous cancer and second leading cause of cancer-related death for men in the United States. The nonprotein coding gene locus plasmacytoma variant translocation 1 (PVT1) is located at 8q24 and is dysregulated in different cancers. PVT1 gives rise to several alternatively spliced transcripts and microRNAs. There are at least twelve exons of PVT1, which make separate transcripts, and likely have different functions. Here, we demonstrate that PVT1 exon 9 is significantly overexpressed in PCa tissues in comparison to normal prostate tissues. Both transient and stable overexpression of PVT1 exon 9 significantly induced greater prostate epithelial cell migration, as well as increased proliferation and corresponding proliferating cell nuclear antigen (PCNA) expression. Notably, implantation into mice of a non-tumorigenic prostate epithelial cell line stably overexpressing PVT1 exon 9 resulted in the formation of malignant tumors. Furthermore, PVT1 exon 9 overexpression significantly induced castration resistance. Consequently, PVT1 exon 9 expression is important for PCa initiation and progression, and holds promise as a therapeutic target in PCa.

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Section: Introductionmentioning

confidence: 99%

Long Noncoding RNA from PVT1 Exon 9 Is Overexpressed in Prostate Cancer and Induces Malignant Transformation and Castration Resistance in Prostate Epithelial Cells

Pal

Huaman

Levine

et al. 2019

Genes

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“…Increasing studies have demonstrated that lncRNAs are associated with the occurrence and development of a variety of cancers . Several lncRNAs, such as UCA1, H19, and PVT1, have been reported in human head and neck carcinoma . To identify the biological function of KCNQ1OT1 in MSSCC, we demonstrated KCNQ1OT1 was upregulated in MSSCC tissues and the IMC‐3 cell line for the first time.…”

Section: Discussionmentioning

confidence: 83%

“…[26][27][28] Several lncRNAs, such as UCA1, H19, and PVT1, have been reported in human head and neck carcinoma. [29][30][31] To identify the biological function of KCNQ1OT1 in MSSCC, we demonstrated KCNQ1OT1 was upregulated in MSSCC tissues and the IMC-3 cell line for the first time. By performing loss-of-function experiments, we discovered KCNQ1OT1 promoted cell proliferation, migration, and invasion in MSSCC.…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA KCNQ1OT1 promotes proliferation, migration, and invasion in maxillary sinus squamous cell carcinoma by regulating miR‐204/EphA7 axis

Sun

et al. 2019

J of Cellular Biochemistry

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Long noncoding RNAs have been demonstrated to contribute to the development and progression of various cancers. However, the underlying regulatory mechanisms of KCNQ1OT1 in tumorigenesis of maxillary sinus squamous cell carcinoma (MSSCC) remain unknown. Herein, we found that KCNQ1OT1 expression was markedly upregulated in MSSCC tissues and MSSCC cell line (IMC‐3) by using quantitative reverse transcription‐polymerase chain reaction. Loss‐of‐function experiments revealed that the deletion of KCNQ1OT1 inhibited cell proliferation, migration, and invasion. Moreover, we confirmed KCNQ1OT1 could directly interact with miR‐204 by bioinformatic prediction and dual luciferase assay, and miR‐204 inhibitor markedly reversed MSSCC tumor phenotypes induced by shKCNQ1OT1. Finally, we demonstrated that KCNQ1OT1/miR‐204 facilitated MSSCC progression by regulating Eph receptor A7 (EphA7). Taken together, these results revealed a novel regulatory mechanism KCNQ1OT1/miR‐204/EphA7 axis, which could provide a new understanding of MSSCC tumorigenesis and develop potential targets for MSSCC therapy.

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“…All SCCHN tissue samples and clinical information of patients were collected in the same manner as in our previous studies . The study was approved by the Research Ethics Committee of Central South University, Changsha, China.…”

Section: Methodsmentioning

confidence: 99%

“…The western blot assay was carried out according to our previous studies . Antibody information is listed in Table .…”

Section: Methodsmentioning

confidence: 99%

miR‐30e‐5p represses angiogenesis and metastasis by directly targeting AEG‐1 in squamous cell carcinoma of the head and neck

Zhang

Liu

et al. 2019

Cancer Science

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Metastasis is a critical determinant for the treatment strategy and prognosis in patients with squamous cell carcinoma of the head and neck (SCCHN). However, the mechanisms underlying SCCHN metastasis are poorly understood. Our study sought to determine the key microRNA and their functional mechanisms involved in SCCHN metastasis. For The Cancer Genome Atlas (TCGA) data analysis, quantitative PCR was used to quantify the level of miR-30e-5p in SCCHN and its clinical significance was further analyzed. A series of in vitro and in vivo experiments were applied to determine the effects of miR-30e-5p and its target AEG-1 on SCCHN metastasis. A mechanism investigation further revealed that AEG-1 was implicated in the angiogenesis and metastasis mediated by miR-30e-5p. Overall, our study confirms that miR-30e-5p is a valuable predictive biomarker and potential therapeutic target in SCCHN metastasis. K E Y W O R D SAEG-1, angiogenesis, metastasis, miR-30e-5p, squamous cell carcinoma of the head and neck

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LncRNA PVT1 promotes malignant progression in squamous cell carcinoma of the head and neck

Cited by 25 publications

References 47 publications

Long Noncoding RNA from PVT1 Exon 9 Is Overexpressed in Prostate Cancer and Induces Malignant Transformation and Castration Resistance in Prostate Epithelial Cells

Long Noncoding RNA from PVT1 Exon 9 Is Overexpressed in Prostate Cancer and Induces Malignant Transformation and Castration Resistance in Prostate Epithelial Cells

Long noncoding RNA KCNQ1OT1 promotes proliferation, migration, and invasion in maxillary sinus squamous cell carcinoma by regulating miR‐204/EphA7 axis

miR‐30e‐5p represses angiogenesis and metastasis by directly targeting AEG‐1 in squamous cell carcinoma of the head and neck

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