LncRNA PVT1 predicts prognosis and regulates tumor growth in prostate cancer

Abstract

Long non-coding RNA (lncRNA) plasmacytoma variant translocation 1(PVT1) was aberrantly expressed in various cancers and is associated with tumor prognosis. Here, we aim to investigate its function in prostate cancer. Small interfering RNA against PVT1 was transfected into prostate cancer cell lines and cell growth and apoptosis were analyzed. Our results showed that PVT1 was overexpressed in prostate cancer tissues and cells. Higher levels of PVT1 indicated poorer overall survival and disease-free survival. A significant association was found between PVT1 expression and tumor stage. Besides, PVT1 knockdown significantly inhibited prostate cancer growth in vivo and in vitro and promoted cell apoptosis. PVT1 knockdown also significantly upregulated the expression of cleaved caspase-3 and cleaved caspase-9, but downregulated the expression of c-Myc in prostate cancer cell lines. Our results suggest that PVT1 played an oncogenic role in prostate cancer and could be used as a potential biomarker for diagnosis of prostate cancer.

Mutational Profile of Aggressive, Localised Prostate Cancer from African Caribbean Men Versus European Ancestry Men

“…2). Concordantly, Yang et al [10] reported recently that higher levels of this lncRNa were associated with poorer overall survival and disease-free survival. Moreover, they showed that PVT1 knockdown by small interfering RNA transfection significantly inhibited PCa growth in vivo and in vitro and promoted cell apoptosis, suggesting that PVT1 played an oncogenic role in PCa.…”

Long Noncoding RNA from PVT1 Exon 9 Is Overexpressed in Prostate Cancer and Induces Malignant Transformation and Castration Resistance in Prostate Epithelial Cells

“…Downregulation of expression of the long noncoding RNA (lncRNA) from PVT1 inhibits proliferation and migration by regulating p38 expression in PCa [19]. Increasing evidence has shown the important role of lncRNAs in many different biological processes [23][24][25][26][27][28][29][30][31]. PVT1 itself has been implicated in cell proliferation, apoptosis, lymph node invasion, metastasis, and tumor prognosis [23].…”

Long non-coding RNA PVT1 interacts with MYC and its downstream molecules to synergistically promote tumorigenesis

“…A high PVT1 expression level in patients with prostate cancer has been shown to be associated with low overall survival. PVT1 expression is significantly correlated with tumor stage and can promote tumor cell proliferation, invasion, and metastasis in prostate cancer [128,144]. The expression of PVT1 in ovarian cancer tissues is higher than that in normal ovarian tissue and is related to the advanced stage of ovarian cancer and lower overall survival.…”

“…PVT1 can downregulate the expression of caspase-9, caspase-7, and poly ADP-ribose polymerase, thereby inhibiting apoptosis and ultimately leading to radiation tolerance [14]. Studies have also shown that PVT1 can downregulate the expression of caspase-3 to inhibit apoptosis [143,144]. Therefore, PVT1 may participates in the downstream apoptosis-related signaling pathway of c-Myc by affecting MDM, caspase-9, and caspase-3 ( Fig.…”