LncRNA PVT1 Facilitates Proliferation, Migration and Invasion of NSCLC Cells via miR-551b/FGFR1 Axis

Abstract

Background Long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) plays a crucial role in non-small cell lung cancer (NSCLC). Nonetheless, regulatory effects of PVT1 on functions of NSCLC cells remain blurry. Methods Relative expression levels of PVT1, miR-551b and FGFR1 mRNA in tumor tissues and cells were examined employing quantitative real-time polymerase chain reaction (qRT-PCR); CCK-8 and BrdU assays were utilized for measuring cell viability and proliferation of H1299 and A549 cells; cell migration and invasion were detected deploying Transwell assay; dual-luciferase assay was used for the validation of binding sequence between PVT1 and miR-551b. FGFR1 expression in protein level was quantified employing Western blot. Results PVT1 was highly expressed in NSCLC tissues and cell lines, whereas miR-551b expression was down-regulated. Overexpression of PVT1 potentiated viability, proliferation, migration and invasion of NSCLC cells while miR-551b inhibited the biological behaviors mentioned above. MiR-551b was predicted and then confirmed as a direct downstream target of PVT1. Meanwhile, a negative correlation was observed between PVT1 expression and miR-551b expression in NSCLC tissues. Besides, PVT1 could increase FGFR1 expression by repressing miR-551b expression. Conclusion PVT1 promotes the proliferation, migration and invasion of NSCLC cells by indirectly mediating FGFR1 via targeting miR-551b.

lncRNA PVT1: a novel oncogene in multiple cancers

“…Specifically, PVT1 reportedly contributed to the proliferation, migration, invasion, and apoptosis of NSCLC cells by upregulating VEGF, VEGFC, SOX9, interleukin-6, BAMBI, RAB34, fibroblast growth factor receptor 1 (FGFR1), and SLC2A1 via miR-29c, miR-128, miR-361-3p, miR-760, miR-17-5p, miR-148, miR-551b, and miR-378c regulation (Fig. 1H−O) [61][62][63][64][65][66][67][68], respectively.…”

Long non-coding RNA PVT1: A promising chemotherapy and radiotherapy sensitizer

“…LncPVT1 is highly expressed in non-small cell lung cancer (NSCLC) and is associated with poor prognosis (91). Wang et al found that lncPVT1 facilitates the proliferation, migration, and invasion of NSCLC cells by indirectly mediating FGFR1 via targeting miR-551b (92). In another study, lncPVT1 activates the Wnt/b-catenin signaling pathway by miR-361-3p/SOX9 axis, thereby promoting the proliferation, migration, invasion, and anti-apoptosis of NSCLC cells (93).…”

Effectiveness and Safety of the Traditional Chinese Medicine Treatment (HuoxueHuayu Therapy) for Malignant Tumors: A Systematic Review and Meta-Analysis

“…(3) randomized controlled trial (RCT). Exclusion criteria were (1) animal studies; (2) retrospective studies; (3) protocols; (4) studies without HuoxueHuayu therapy; (5) systematic reviews and meta-analyses; (6) abstract alone; (7) not cancer or irrelevant subject; (8) incomplete data; (9) duplicate articles.…”

“…Although signi cant advances in diagnostic screening, surgical resection, and targeted therapies have been made in recent years, the overall situation of malignant tumor treatment is still not optimistic. For instance, patients who are diagnosed at the middle or advanced stages [4,5] seem to miss the optimum period for surgery [6]. Hence, nonsurgical treatments, such as chemotherapy, radiation therapy, or other treatments (nanomedicines) [7], still play a crucial role in the treatment of middle and advanced malignant tumors.…”