LncRNA PCAT18 Promotes Non-Small Cell Lung Cancer Progression by Sponging miR-4319

Abstract

Introduction NSCLC (non-small cell lung cancer), the most common type of human cancer, is a main cause of cancer-associated mortality. Accumulating evidence has confirmed that long non-coding RNAs serve crucial roles in NSCLC development. Methods The PCAT18 expression in NSCLC tissues and cell lines were evaluated by reverse transcription-quantitative PCR. Cell Counting Kit-8 assays, colony formation study, wound healing assays and transwell invasion assays, and tumor xenograft experiments were performed to investigate the biological functions of PCAT18 in NSCLC. Luciferase reporter, RNA-binding protein immunoprecipitation (RIP) and RNA pull-down assays were further used to explore the association between PCAT18 and miR-4319. Results PCAT18 expression was up-regulated in NSCLC tissues and cell lines. Furthermore, PCAT18 silencing inhibited NSCLC cell proliferation, migration and invasion, while co-transfection with a miR-4319 inhibitor reversed these biological effects, and miR-4319 inhibited NSCLC growth in vivo. Additionally, PCAT18 silencing promoted NSCLC cell apoptosis and induced G1 stage arrest. Moreover, luciferase reporter assays illustrated that PCAT18 regulated miR-4319 directly, and a RIP assay and RNA pull-down analysis further demonstrated that miR-4319 inhibited PCAT18 in a RNA-induced silencing complex-dependent manner. Finally, PCAT18 silencing impaired the growth of NSCLC in vivo. Conclusion In conclusion, these findings demonstrated that PCAT18 promoted NSCLC development by sponging miR-4319. PCAT18 may serve as a crucial biomarker for the diagnosis and targeted therapy of NSCLC.

Bulk and single-cell RNA sequencing reveal the contribution of laminin γ2 -CD44 to the immune resistance in lymphocyte-infiltrated squamous lung cancer subtype

“…S3A and B ). Among these genes, IL17REL, KLRC2, MGAM2 [ 51 ], and XAGE1B [ 52 ] are associated with the immune response and immunotherapy, whereas PCAT18, TUSC8, and GPR31 have been reported to be therapeutic targets in tumors [ [53] , [54] , [55] ]. Additionally, overall survival analysis of these genes in CS3 patients found that C11orf86 and LINC02539 were significantly correlated with prognosis in patients with the CS3 subtype, whereas IL17REL, KLRC2, INSM1, and XAGE1B were potential prognostic factors ( Fig.…”

Upregulation of ENKD1 disrupts cellular homeostasis to promote lymphoma development

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“…SDHAP1 was involved in the proliferation, migration, and invasion of NSCLC (L. He et al, 2021). PTGIS and EARS2 were demonstrated to regulate colon cancer metastasis (Cui et al, 2021;Sun et al, 2012).…”

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