LncRNA NALT interaction with NOTCH1 promoted cell proliferation in pediatric T cell acute lymphoblastic leukemia

Wang, Yaping; Wu, Peng; Lin, Rufeng; Rong, Liucheng; Xue, Yue; Fang, Yongjun

doi:10.1038/srep13749

Cited by 89 publications

(69 citation statements)

References 38 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Identification of effective disease biomarkers for prognosis is urgently required. In recent years, mounting epidemiological and molecular biological studies have demonstrated that lncRNAs dysregulation was involved in causing cancers [19,20]. By using detection analysis such as RNA sequencing and microarray, altered expression of lncRNAs has been reported in varies of malignancies, and multiple deregulated lncRNAs have been implicated in physiological and pathological processes of human cancer [21][22][23][24].…”

Section: Discussionmentioning

confidence: 99%

Four Circulating Long Non-Coding RNAs Act as Biomarkers for Predicting Cervical Cancer

Sun

Wang

Zhao

et al. 2018

Gynecol Obstet Invest

View full text Add to dashboard Cite

Background/Aims: Circulation long non-coding RNAs (lncRNAs) have emerged recently as major players in tumor biology and might be applied for cancer diagnosis, prognosis, or potential therapeutic targets. In this study, we aimed to explore whether the circulation lncRNA could predict the tumorigenesis of surgical squamous cervical cancer (CC). Methods: In this study, we applied the lncRNA microarray to screen the potential biomarker for CC. Real-time quantitate polymerase chain reaction was conducted for further validation in a larger sample size. The multi-stage validation and risk score formula analysis were used to examine the sensitivity and specificity. Results: We discovered 4 lncRNAs including HOTAIR, PVT1, XLOC_000303, and AL592284.1, which were upregulated in CC comparing with the cancer-free controls. Further, the receiver operating characteristic curve (ROC) analysis by risk score formula revealed the combined 4 factors with a high diagnostic ability with the area under ROC curve value of 0.875 and 0.958 in training set and validation set respectively. We finally confirmed the stable detection of the 4 lncRNAs by 5 cycles of freezing and thawing. Conclusion: HOTAIR, PVT1, XLOC_000303, and AL592284.1 might be the potential biomarkers for predicting the tumorigenesis of CC in the future.

show abstract

Section: Discussionmentioning

confidence: 99%

Four Circulating Long Non-Coding RNAs Act as Biomarkers for Predicting Cervical Cancer

Sun

Wang

Zhao

et al. 2018

Gynecol Obstet Invest

View full text Add to dashboard Cite

show abstract

“…LncRNAs have tissue-specific expression and serve an important role in the human transcriptome by regulating normal tissue differentiation as well as cancer development (30). Notably, a number of previous studies have implicated a role of lncRNA dysregulation, of transcripts including leukemia-induced non-coding activator RNA, B-ALL-associated long RNA (BALR)-6 and -2, NOTCH1-associated lncRNA in T-ALL and CCDC26, in tumorigenicity in leukemias (31)(32)(33)(34). SNPs may significantly influence gene expression and function.…”

Section: Discussionmentioning

confidence: 99%

Long non‑coding RNA PAX8‑AS1 polymorphisms increase the risk of childhood acute lymphoblastic leukemia

et al. 2017

View full text Add to dashboard Cite

Abstract. The present case-control study was conducted on 110 children with acute lymphoblastic leukemia (ALL) and 120 healthy children to determine the impact of polymorphisms in paired-box gene 8 (PAX8) antisense RNA 1 (PAX8-AS1), namely rs4848320 C>T, rs6726151 T>G and rs1110839 G>T, on ALL risk. Genotyping was performed through the polymerase chain reaction-restriction fragment length polymorphism method. The findings indicated that the rs4848320 variant increased the risk of ALL in codominant [CT vs. CC: odds ratio (OR)=2.13, 95% confidence interval (CI)=1.16-3.90, P=0.014; and TT vs. CC: OR=2.21, 95% CI=1.03-4.74, P=0.041], dominant (CT+TT vs. CC: OR=2.15, 95% CI=1.22-3.81, P=0.009,) and allele (T vs. C: OR=1.55, 95% CI=1.07-2.25, P=0.024) inheritance models. The rs6726151 variant significantly increased the risk of ALL in codominant (GT vs. GG: OR=1.88, 95% CI=1.08-3.27, P=0.036) and overdominant (GT vs. GG+TT: OR=2.08, 95% CI=1.23-3.53, P=0.008) inheritance models. No significant relationship was identified between the rs1110839 G>T variant and disease risk/protection in childhood ALL. In conclusion, the findings of the present study indicated that rs4848320 and rs6726151 polymorphisms of PAX8-AS1 may be a risk factor for the development of childhood ALL. Further studies with larger sample sizes and different ethnicities are now required to confirm these findings.

show abstract

“…U6 snRNA was used as an internal control. The primers for detecting the expression of CYLD were 5ʹ-TCAGGCTTATGGAGCCAAGAA-3ʹand 5ʹ-ACTTCCCTTCGGTACTTTAAGGA-3ʹ.QRT-PCR was performed using an ABI Prism 7900HT instrument (Applied Biosystems) according to the directions provided with the reagents [22].…”

Section: Quantitative Real Time Polymerase Chain Reaction (Qrt-pcr)mentioning

confidence: 99%

A Function Variant at miR-501 Alters Susceptibility to Hepatocellular Carcinoma in a Chinese Han Population

Liu

Chai

Zhang

et al. 2016

Cell Physiol Biochem

View full text Add to dashboard Cite

Backgroud/Aims: Previous studies have shown that miR-501 is involved in the development of hepatocellular carcinoma (HCC) by promoting cell proliferation through CYLD. From the published MirSNP database that enrolls all single nucleotide polymorphisms(SNPs) of microRNA (miRNA), we found an interesting SNP (rs112489955, G>A) located in the mature region of miR-501. Methods: We performed a case-control study focusing on the predicted SNP located in miRNA-501 to investigate the further relationship of the SNPs with miRNAs among HCC patients. Genotyping, real time PCR assay, cell transfection and the dual luciferase reporter assay were used in our study. Results: Bioinformatic analysis indicated that this SNP would inhibit the binding of miR-501 to CYLD. In a case-control study, subjects with the variant genotypes (AG, GG) showed a significantly increased risk of HCC relative to AA carriers. A significant association of miR-501 variant genotypes with enhanced tumor growth was also observed. Further functional analyses indicated that patients with the AA genotype might attenuate the level of CYLD compared to that regulated by miR-501 with the GG genotype. A dual luciferase reporter assay also confirmed that miR-501 with the A allele had reduced binding to CYLD. We further confirmed a suppression of cell proliferation and promotion of apoptosis in SMMC-7721 and Hep3B cell lines treated with the AA genotype. Conclusions: We identified a novel SNP located in miR-501 acting as an important factor of the HCC susceptibility by modulating miR-501 and CYLD levels.

show abstract

LncRNA NALT interaction with NOTCH1 promoted cell proliferation in pediatric T cell acute lymphoblastic leukemia

Cited by 89 publications

References 38 publications

Four Circulating Long Non-Coding RNAs Act as Biomarkers for Predicting Cervical Cancer

Four Circulating Long Non-Coding RNAs Act as Biomarkers for Predicting Cervical Cancer

Long non‑coding RNA PAX8‑AS1 polymorphisms increase the risk of childhood acute lymphoblastic leukemia

A Function Variant at miR-501 Alters Susceptibility to Hepatocellular Carcinoma in a Chinese Han Population

Contact Info

Product

Resources

About