LncRNA MT1JP functions as a ceRNA in regulating FBXW7 through competitively binding to miR-92a-3p in gastric cancer

Abstract

BackgroundEmerging evidence has shown that dysregulation function of long non-coding RNAs (lncRNAs) implicated in gastric cancer (GC). However, the role of the differentially expressed lncRNAs in GC has not fully explained.MethodsLncRNA expression profiles were determined by lncRNA microarray in five pairs of normal and GC tissues, further validated in another 75 paired tissues by quantitative real-time PCR (qRT-PCR). Overexpression of lncRNA MT1JP was conducted to assess the effect of MT1JP in vitro and in vivo. The biological functions were demonstrated by luciferase reporter assay, western blotting and rescue experiments.ResultsLncRNA MT1JP was significantly lower in GC tissues than adjacent normal tissues, and higher MT1JP was remarkably related to lymph node metastasis and advance stage. Besides, GC patients with higher MT1JP expression had a well survival. Functionally, overexpression of lncRNA MT1JP inhibited cell proliferation, migration, invasion and promoted cell apoptosis in vitro, and inhibited tumor growth and metastasis in vivo. Functional analysis showed that lncRNA MT1JP regulated FBXW7 expression by competitively binding to miR-92a-3p. MiR-92a-3p and down-regulated FBXW7 reversed cell phenotypes caused by lncRNA MT1JP by rescue analysis.ConclusionMT1JP, a down-regulated lncRNA in GC, was associated with malignant tumor phenotypes and survival of GC. MT1JP regulated the progression of GC by functioning as a competing endogenous RNA (ceRNA) to competitively bind to miR-92a-3p and regulate FBXW7 expression. Our study provided new insight into the post-transcriptional regulation mechanism of lncRNA MT1JP, and suggested that MT1JP may act as a potential therapeutic target and prognosis biomarker for GC.Electronic supplementary materialThe online version of this article (10.1186/s12943-018-0829-6) contains supplementary material, which is available to authorized users.

Retracted: LncRNA MT1JP functions as a tumor suppressor via regulating miR‐214‐3p expression in bladder cancer

“…Previous references proved that MT1JP has been played a tumor suppressor gene in a lot of tumors (L. H. Liu, Yue, et al, 2016; Yang et al, ; G. Zhang et al, ; Zhu, Ma, et al, ). For instance, L. H. Liu, Yue, et al () first indicated that MT1JP expression was downregulated in the cancer tissues and downregulation expression of MT1JP promoted cancer cell deterioration and cancer formation through regulating p53 expression via interactions with the TIAR.…”

Long non-coding RNA ARAP1-AS1 promotes the progression of bladder cancer by regulating miR-4735-3p/NOTCH2 axis

“…As reported previously, lncRNAs can modulate gene expression in human cancers by acting as ceRNAs. [26][27][28][29][30] Here, we designed mechanism experiments to detect whether ARAP1-AS1 can act as a ceRNA in BCa. The localization of ARAP1-AS1 was detected in BCa cells.…”

<p>lncRNA MALAT1 modulates oxaliplatin resistance of gastric cancer via sponging miR-22-3p</p>

“…miRNAs and lncRNAs belong to noncoding RNAs and increasing evidence demonstrated that lncRNA can act as competing endogenous RNAs (ceRNAs) to regulate mRNA by binding their common miRNAs in various cancers. [17][18][19][20] These regulatory networks are widely involved in tumor occurrence, development, apoptosis, and drug resistance in GC. For instance, HOTAIR contributed to cisplatin resistance by regulating VEGFA and PIK3R2 via targeting miR-126 in GC.…”