LncRNA MSC-AS1 promotes osteogenic differentiation and alleviates osteoporosis through sponging microRNA-140–5p to upregulate BMP2

Zhang, Naidong; Hu, Xinyu; He, Shikun; Ding, Wenge; Wang, Feng; Zhao, Yichao; Huang, Zhihui

doi:10.1016/j.bbrc.2019.09.058

Cited by 77 publications

(52 citation statements)

References 40 publications

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“…lncRNA has been shown to interact with miRNAs to regulate tumor cell progression, and MSC-AS1 was validated to modulate osteogenic differentiation and osteoporosis by sponging miRNA-140-5p [14]. We speculated whether MSC-AS1 can interact with other miRNAs to regulate TMZ resistance of glioma cells.…”

Section: Discussionmentioning

confidence: 99%

“…lncRNA MSC-AS1 (MSC-AS1) has been found to be up-regulated in kidney renal clear cell carcinoma [12] and pancreatic ductal adenocarcinoma [13] and plays a carcinogenic role. More than that, MSC-AS1 could facilitate osteogenic differentiation and attenuate osteoporosis via binding to miR-140-5p [14]. However, the expression pattern and mechanism of MSC-AS1 in glioma remain unknown, let alone the regulation of TMZ resistance.…”

Section: Introductionmentioning

confidence: 99%

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MSC-AS1 knockdown inhibits cell growth and temozolomide resistance by regulating miR-373-3p/CPEB4 axis in glioma through PI3K/Akt pathway

Feng

Chen

2020

Mol Cell Biochem

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Long non-coding RNAs (lncRNAs) have been widely reported to regulate the development and chemoresistance of a variety of tumors. Temozolomide (TMZ) is a first-line chemotherapy for treatment of glioma. However, the effect and the regulatory mechanism of lncRNA MSC-AS1 (MSC-AS1) in TMZ-resistant glioma remain unrevealed. Levels of MSC-AS1, microRNA-373-3p (miR-373-3p), and cytoplasmic polyadenylation element binding protein 4 (CPEB4) were determined by quantitative real-time polymerase chain reaction (qRT-PCR). All protein expression was detected by western blot. Cell viability and the half maximal inhibitory concentration (IC50) value of TMZ was assessed by cell counting kit-8 (CCK-8) assay. Cell cloning ability and apoptosis were examined by colony formation and flow cytometry assays, respectively. Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays were performed to verify the correlation between miR-373-3p and MSC-AS1 or CPEB4. The xenograft models were established to determine the effect of MSC-AS1 in vivo. MSC-AS1 was up-regulated in TMZ-resistant glioma tissues and cells, and glioma patients with high MSC-AS1 expression tend to have lower overall survival rate. MSC-AS1 suppression reduced the IC50 value of TMZ and proliferation, promoted apoptosis and TMZ sensitivity, and affected PI3K/Akt pathway in TMZ-resistant glioma cells. MSC-AS1 acted as miR-373-3p sponge, and miR-373-3p directly targeted CPEB4. Silencing miR-373-3p reversed the promoting effect of MSC-AS1 or CPEB4 knockdown on TMZ sensitivity. Furthermore, MSC-AS1 knockdown inhibited TMZ-resistant glioma growth in vivo by regulating miR-373-3p/CPEB4 axis through PI3K/Akt pathway. Collectively, MSC-AS1 knockdown suppressed cell growth and the chemoresistance of glioma cells to TMZ by regulating miR-373-3p/CPEB4 axis in vitro and in vivo through activating PI3K/Akt pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MSC-AS1 knockdown inhibits cell growth and temozolomide resistance by regulating miR-373-3p/CPEB4 axis in glioma through PI3K/Akt pathway

Feng

Chen

2020

Mol Cell Biochem

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“…lncRNA MSC-AS1 (MSC antisense RNA 1) may alleviate osteoporosis. Expression of MSC-AS1 was found to increase with osteogenic differentiation of mice BMSCs, as well as osteogenesis-related genes, such as RUNX2, osteopontin, and osteocalcin [ 64 ]. Knockdown of MSC-AS1, on the other hand, downregulated BMP2, p-smad1/5/8, and RUNX2 [ 64 ].…”

Section: The Role Of Micro Rna (Mirna) and Long-non-coding Rna (Lnmentioning

confidence: 99%

The Role of Micro RNA and Long-Non-Coding RNA in Osteoporosis

Chen

2020

IJMS

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Osteoporosis is a major concern worldwide and can be attributed to an imbalance between osteoblastic bone formation and osteoclastic bone resorption due to the natural aging process. Heritable factors account for 60–80% of optimal bone mineralization; however, the finer details of pathogenesis remain to be elucidated. Micro RNA (miRNA) and long-non-coding RNA (lncRNA) are two targets that have recently come into the spotlight due to their ability to control gene expression at the post-transcriptional level and provide epigenetic modification. miRNAs are a class of non-coding RNAs that are approximately 18–25 nucleotides long. It is thought that up to 60% of human protein-coding genes may be regulated by miRNAs. They have been found to regulate gene expression that controls osteoblast-dependent bone formation and osteoclast-related bone remodeling. lncRNAs are highly structured RNA transcripts longer than 200 nucleotides that do not translate into proteins. They have very complex secondary and tertiary structures and the same degradation processes as messenger RNAs. The fact that they have a rapid turnover is due to their sponge function in binding the miRNAs that lead to a degradation of the lncRNA itself. They can act as signaling, decoy, and framework molecules, or as primers. Current evidence suggests that lncRNAs can act as chromatin and transcriptional as well as post-transcriptional regulators. With regards to osteoporosis, lncRNA is thought to be involved in the proliferation, apoptosis, and inflammatory response of the bone. This review, which is based on a systematic appraisal of the current literature, provides current molecular and genetic opinions on the roles of miRNAs and lncRNAs in osteoporosis. Further research into the epigenetic modification and the regulatory roles of these molecules will bring us closer to potential disease-modifying treatment for osteoporosis. However, more issues regarding the detailed actions of miRNAs and lncRNAs in osteoporosis remain unknown and controversial and warrant future investigation.

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“…Peng et al (2018) showed that anti-differentiation ANCR plays as a competitive endogenous RNA (ceRNA) functions as a suppressor of osteogenic differentiation through via sponging miRNA-758. Zhang et al (2019) found that lncRNA MSC-AS1 positively regulated osteogenic differentiation of BMSCs by acting as a ceRNA to regulate BMP2 expression through sponging miR-140-5p. These positive and negative regulations of lncRNAs indicate a great role in controlling the differentiation state of stem cells.…”

Section: Introductionmentioning

confidence: 99%

LncRNA LOC100506178 promotes osteogenic differentiation via regulating miR-214-5p-BMP2 axis in human bone marrow mesenchymal stem cells

Fang

Liu

et al. 2020

PeerJ

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Osteogenic differentiation is an important role in dental implantation. Long no coding RNAs (lncRNAs) are a novel class of noncoding RNAs that have significant effects in a variety of diseases. However, the function and mechanisms of LOC100506178 in osteogenic differentiation and migration of bone morphogenetic protein 2 (BMP2)induced osteogenic differentiation of human bone marrow mesenchymalstem cells (hBMSCs) remain largely unclear. BMP2 was used to induce osteogenic differentiation of hBMSCs. Quantitative real time PCR (qRT-PCR) was used to examine the expression of LOC100506178, miR-214-5p, Runt-related transcription factor 2 (RUNX2), Osterix (Osx), and Alkaline Phosphatase (ALP) in BMP2-induced osteogenic differentiation of hBMSCs. The function of LOC100506178 and miR-214-5p was explored in vitro using Alizarin Red S Staining, ALP activity, as well as in vivo ectopic bone formation. Luciferase reporter assay was performed to assess the association between LOC100506178 and miR-214-5p, as well as miR-214-5p and BMP2. The miR-214-5p sponging potential of LOC100506178 was evaluated by RNA immunoprecipitation. In the present study, the expression of LOC100506178 was found to be increased in BMP2induced osteogenic differentiation of hBMSCs, accompanied with decreased miR-214-5p expression and increased RUNX2, Osx and ALP expression. LOC100506178 significantly induced, while miR-214-5p suppressed the BMP2-induced osteogenic differentiation of hBMSCs. Mechanistically, LOC100506178 was directly bound to miR-214-5p and miR-214-5p targeted the 3 -untranslated region of BMP2 to negatively regulate its expression. In conclusion, our data indicate a novel molecular pathway LOC100506178/miR-214-5p/BMP2 in relation to hBMSCs differentiation into osteoblasts, which may facilitate bone anabolism. Subjects Biochemistry, Cell Biology How to cite this article Li L, Fang J, Liu Y, Xiao L. 2020. LncRNA LOC100506178 promotes osteogenic differentiation via regulating miR-214-5p-BMP2 axis in human bone marrow mesenchymal stem cells. PeerJ 8:e8909 http://doi.org/10.7717/peerj.8909 Li et al. (2020), PeerJ, DOI 10.7717/peerj.8909 2/15 Li et al. (2020), PeerJ, DOI 10.7717/peerj.8909 3/15 Li et al. (2020), PeerJ, DOI 10.7717/peerj.8909 7/15 Li et al. (2020), PeerJ, DOI 10.7717/peerj.8909 9/15 Li et al. (2020), PeerJ, DOI 10.7717/peerj.8909 10/15 Li et al. (2020), PeerJ, DOI 10.7717/peerj.8909 12/15 Al-Almaie S, Kavarodi AM, Al Faidhi A. 2013. Maxillary sinus functions and complications with lateral window and osteotome sinus floor elevation procedures followed by dental implants placement: a retrospective study in 60 patients. . 2016. MiR-34a promotes osteogenic differentiation of human adipose-derived stem cells via the RBP2/NOTCH1/CYCLIN D1 coregulatory network. Stem Cell Reports 7:236-248 Sang Q. 2017. miR-214 suppresses the osteogenic differentiation of bone marrow-derived mesenchymal stem cells and these effects are mediated through the inhibition of the JNK and p38 pathways. Du J, Fan Z. 2013. Shh signaling, negatively regulated by...

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LncRNA MSC-AS1 promotes osteogenic differentiation and alleviates osteoporosis through sponging microRNA-140–5p to upregulate BMP2

Cited by 77 publications

References 40 publications

MSC-AS1 knockdown inhibits cell growth and temozolomide resistance by regulating miR-373-3p/CPEB4 axis in glioma through PI3K/Akt pathway

MSC-AS1 knockdown inhibits cell growth and temozolomide resistance by regulating miR-373-3p/CPEB4 axis in glioma through PI3K/Akt pathway

The Role of Micro RNA and Long-Non-Coding RNA in Osteoporosis

LncRNA LOC100506178 promotes osteogenic differentiation via regulating miR-214-5p-BMP2 axis in human bone marrow mesenchymal stem cells

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