LncRNA MEG3-TRPV1 signaling regulates chronic inflammatory pain in rats

Zhang, Hai-Long; Peng, Jing-Wei; Gu, Yin-Yin; Wang, Jia; Sun, Ye; Zhu, Chun-Long; Song, Yu; Chen, Long; Zhang, Ling; Chen, Xia; Wang, Qian

doi:10.1177/17448069221144246

Cited by 4 publications

(2 citation statements)

References 40 publications

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“…Previous studies [ 42 ] have detailed the procedure for performing Western blotting. The quantification of NKCC1/KCC2 expression in the PFCs of 4-week-old mice was accomplished through Western blotting analysis.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of NKCC1 Ameliorates Anxiety and Autistic Behaviors Induced by Maternal Immune Activation in Mice

Zhang,

Hu,

et al. 2024

CIMB

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Autism spectrum disorder (ASD) is thought to result from susceptibility genotypes and environmental risk factors. The offspring of women who experience pregnancy infection have an increased risk for autism. Maternal immune activation (MIA) in pregnant animals produces offspring with autistic behaviors, making MIA a useful model for autism. However, how MIA causes autistic behaviors in offspring is not fully understood. Here, we show that NKCC1 is critical for mediating autistic behaviors in MIA offspring. We confirmed that MIA induced by poly(I:C) infection during pregnancy leads to autistic behaviors in offspring. We further demonstrated that MIA offspring showed significant microglia activation, excessive dendritic spines, and narrow postsynaptic density (PSD) in their prefrontal cortex (PFC). Then, we discovered that these abnormalities may be caused by overexpression of NKCC1 in MIA offspring’s PFCs. Finally, we ameliorated the autistic behaviors using PFC microinjection of NKCC1 inhibitor bumetanide (BTN) in MIA offspring. Our findings may shed new light on the pathological mechanisms for autism caused by pregnancy infection.

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Section: Methodsmentioning

confidence: 99%

Inhibition of NKCC1 Ameliorates Anxiety and Autistic Behaviors Induced by Maternal Immune Activation in Mice

Zhang,

Hu,

et al. 2024

CIMB

Self Cite

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“…The progression of OA can lead to the activation and overexpression of TRPV1. Lowering TRPV1 expression could help alleviate chronic inflammatory pain, providing potential therapeutic targets for osteoarthritis patients [16]. Additionally, the activation of TRPV1 and the release of CGRP have been observed in the DRG of a mouse pain model.…”

Section: Introductionmentioning

confidence: 99%

Suppression of CGRP and TRPV1 Expression in Dorsal Root Ganglia of Knee Osteoarthritis Rats by Huojing Decoction via TrkA/MKK3/6/p38 Pathway

Jiang,

Guo,

Fang

et al. 2024

Preprint

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Background Knee osteoarthritis (KOA) is a chronic condition characterized by persistent pain that can lead to severe disability. In this study, we primarily investigated the analgesic effect of Huojing decoction on MIA-induced knee arthritis. Methods MIA was injected to induce KOA pain in rats. Huojing decoction was then administered once daily via intragastric administration for 14 days. Pain level was assessed by measuring the paw withdrawal threshold (PWT) and paw withdrawal latency (PWL). ELISA determined serum levels of TNF-α, IL-1β, and IL-6. The Dorsal Root Ganglia (DRG) of L4 and L5 were collected from rats. TRPV1 and CGRP were detected through immunohistochemistry and immunofluorescence. The protein expression of TrkA, MKK3/6, and p38 was assessed by Western blot, and q-PCR measured the mRNA expression of TNF-α, IL-1β, and IL-6. Results Mechanical allodynia and thermal hyperalgesia were observed in KOA rats. The expression levels of inflammatory cytokines IL-1β, IL-6, and TNF-α were significantly decreased after Huojing decoction administration of KOA rats. Furthermore, administration of Huojing decoction in rats significantly decreased the mRNA expression levels of IL-1β, IL-6, and TNF-α in DRG. TRPV1 and CGRP levels were reduced with treatment. Conversely, the protein expressions of TrkA, MKK3/6, and p38 in the DRG of rats were significantly decreased. Conclusion Our data suggested that Huojing decoction can alleviate inflammation in KOA pain rats. Additionally, it can inhibit the expression of TrKA, MKK3/6, and p38 signaling pathways, indicating its analgesic effect on KOA pain rats.

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LncRNAs and PTEN/PI3K signaling: A symphony of regulation in cancer biology

Almalki

2023

Pathology - Research and Practice

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LncRNA MEG3-TRPV1 signaling regulates chronic inflammatory pain in rats

Cited by 4 publications

References 40 publications

Inhibition of NKCC1 Ameliorates Anxiety and Autistic Behaviors Induced by Maternal Immune Activation in Mice

Inhibition of NKCC1 Ameliorates Anxiety and Autistic Behaviors Induced by Maternal Immune Activation in Mice

Suppression of CGRP and TRPV1 Expression in Dorsal Root Ganglia of Knee Osteoarthritis Rats by Huojing Decoction via TrkA/MKK3/6/p38 Pathway

LncRNAs and PTEN/PI3K signaling: A symphony of regulation in cancer biology

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