LncRNA MEG3 functions as a ceRNA in regulating hepatic lipogenesis by competitively binding to miR-21 with LRP6

Huang, Peng; Huang, Feizhou; Liu, Huai-Zheng; Zhang, Tianyi; Yang, Mingshi; Sun, Chuanzheng

doi:10.1016/j.metabol.2019.01.018

Cited by 71 publications

(63 citation statements)

References 32 publications

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“…In the present study, we have discovered that: Lnc-MEG3, an imprinted gene, is transcribed from human chromosome 14q32.3 that is implied in the induction of inflammatory response and multiple organ dysfunction such as heart. 8,17,18 For instance, the overexpression of lnc-MEG3 enhances the expressions and secretions of inflammatory cytokines such as IL-1β, IL-6, IL-8, and TNF-α in osteoarthritis. 17 Another study discloses that lnc-MEG3 promotes apoptosis in cardiomyocytes via direct binding with fused in sarcoma protein in myocardial infarction.…”

Section: Discussionmentioning

confidence: 99%

“…18 Besides, it is evident that lnc-MEG3 directly binds to miR-21 and subsequently inhibits its downstream signaling pathways. 7,8 As for miR-21, it has been proposed as a regulator that elicits effect on inhibiting inflammatory responses and protecting several vital organs from damage in sepsis. 10,11 Based on the effects of lnc-MEG3 and miR-21 on inflammation and organ injury as well as the interaction between lnc-MEG3 and miR-21, we speculated that and alleviated tissue damage as well as organ dysfunctions, thereby, leading to lower levels of inflammation and attenuated disease severity in sepsis patients.…”

Section: Discussionmentioning

confidence: 99%

“…6 Furthermore, it is reported that lnc-MEG3 acts as a molecular sponge for several microRNAs such as microRNA (miR)-21, miR-181a, and miR-7-5p to inhibit their downstream signaling pathways. 5,7,8 Among these target miRNAs, miR-21 functions as an important contributor for preventing inflammation and organ dysfunctions such as liver, kidney, and lung in sepsis. [9][10][11] Based on these previous studies, we hypothesized that lnc-MEG3 might be involved in the development and progression of sepsis via interacting with miR-21.…”

Section: Introductionmentioning

confidence: 99%

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Lnc‐MEG3 acts as a potential biomarker for predicting increased disease risk, systemic inflammation, disease severity, and poor prognosis of sepsis via interacting with miR‐21

Lei

Ding

Xing

et al. 2020

Clinical Laboratory Analysis

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This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. AbstractBackground: This study aimed to investigate the correlations of long non-coding RNA maternally expressed gene 3 (lnc-MEG3), microRNA (miR)-21, and lnc-MEG3/miR-21 axis with disease risk, inflammation, disease severity, and 28-day mortality of sepsis. Methods: Totally, 219 sepsis patients and 219 health controls (HCs) were enrolled. Plasma samples were obtained from sepsis patients within 24 hours after admission and from HCs on enrollment to detect lnc-MEG3 and miR-21 expressions by realtime quantitative polymerase chain reaction. Results: The lnc-MEG3 expression and lnc-MEG3/miR-21 axis were increased, while miR-21 expression was decreased in sepsis patients compared with HCs. Lnc-MEG3 (area under the curve (AUC): 0.887, 95% confidence interval (CI): 0.856-0.917) andlnc-MEG3/miR-21 axis (AUC: 0.934, 95% CI: 0.909-0.958) had good values for predicting elevated sepsis risk, while miR-21 (AUC: 0.801, 95% CI: 0.758-0.844) presented a good predictive value for reduced sepsis risk. Furthermore, lnc-MEG3 expression and lnc-MEG3/miR-21 axis positively correlated with, whereas miR-21 expression negatively correlated with acute pathologic and chronic health evaluation II, sequential organ failure assessment score, serum creatinine, C-reactive protein, tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and IL-17 in sepsis patients. Additionally, lnc-MEG3 (AUC: 0.704, 95% CI: 0.626-0.783) and lnc-MEG3/miR-21 axis (AUC: 0.669, 95% CI: 0.589-0.750) exhibited acceptable values in predicting higher 28-day mortality risk, while miR-21 (AUC: 0.588, 95% CI: 0.505-0.672) presented a poor predictive value for lower 28-day mortality risk in sepsis patients. Conclusion:Lnc-MEG3 might serve as a potential biomarker for the development, progression, and prognosis prediction of sepsis via interacting with miR-21.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Lnc‐MEG3 acts as a potential biomarker for predicting increased disease risk, systemic inflammation, disease severity, and poor prognosis of sepsis via interacting with miR‐21

Lei

Ding

Xing

et al. 2020

Clinical Laboratory Analysis

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“…In brief, mouse liver tissue and cells were homogenized by RIPA lysis buffer (150 mM Tris-HCl, 50 mM NaCl, 1% NP-40, 0.1% tween- 20), and centrifuged at 15,000 g for 15 min. Then, the supernatant was mixed with loading buffer [125 mM Tris hydrochloride (pH 6.8), 10% mercaptoethanol (vol/vol), 4% SDS (wt/vol), 20% glycerol (vol/vol), and 0.002% bromophenol blue] and then was heated at 100 °C for 10 min.…”

Section: Western Blotting Analysesmentioning

confidence: 99%

“…Emerging evidence has revealed that LncRNAs function as important contributors to biological processes underlying the pathophysiology of NAFLD [18][19][20] . For instance, lncRNA MEG3 regulated hepatic lipid mechanism through AKT-mTOR signalling pathway [20] . Long noncoding RNA lncARSR was found to promote hepatic lipogenesis via Akt/SREBP-1c pathway and contribute to the pathogenesis of nonalcoholic steatohepatitis [18] .…”

Section: Suppression Of Gm12664 Expression Decreased Ffa-induced Trigmentioning

confidence: 99%

Long non-coding RNA Gm12664 up-regulates CAV1 expression to promote hepatocellular lipid accumulation by sponging miR-295-5p

Xu¹,

Wang

Chen

et al. 2020

Preprint

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Lnc‐hipk1 inhibits mouse adipocyte apoptosis as a sponge of miR‐497

Yang

Tai

et al. 2021

BioFactors

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Noncoding RNAs (ncRNAs) such as microRNAs (miRNAs), long noncoding RNAs (lncRNA), and circular RNAs are closely related to the biological processes related to obesity. As a miRNA that widely present in different cell types, miR497 is proved to be involved in cell development. However, research on the role of miR-497 as a key factor in regulating the development of adipocytes is still in gap. The role of miR-497 in the apoptosis and proliferation of mouse-derived adipocytes was detected by RNA-seq analysis, RT-qPCR, Western blot, immunofluorescence, and dual-luciferase reporter assay. Using miR-497 mimics to treat 3T3-L1 cells, we found that miR-497 targeted Bcl-2 to promote adipocyte apoptosis through the mitochondrial pathway, and this effect was consistent in the apoptosis model composed of palmitic acid (PA) and hydrogen peroxide (H 2 O 2 ). LncRNA homeodomain-interacting protein kinase 1 (lnc-hipk1) sponged miR-148b to weaken its silencing of Bcl-2, forming the competitive endogenous RNAs (CeRNAs) regulatory network. Furthermore, overexpression of lnc-hipk1 inhibited the apoptosis of adipocytes by targeting miR-497/Bcl-2. Co-treatment of miR-497 and lnc-hipk1 showed that lnc-hipk1 reversed the apoptosis of adipocytes caused by miR-497 overexpression. And in vivo experiments further confirmed that this effect was also achieved by the CeRNA system of lnc-hipk1/miR-497/Bcl-2. In summary, lnc-hipk1 targets miR-497/Bcl-2 to regulate adipocyte apoptosis through the mitochondrial pathway. This research enriches the research content of ncRNAs and CeRNA in adipocyte development, and provides new targets for the treatment of obesity and other metabolic syndromes.

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LncRNA MEG3 functions as a ceRNA in regulating hepatic lipogenesis by competitively binding to miR-21 with LRP6

Cited by 71 publications

References 32 publications

Lnc‐MEG3 acts as a potential biomarker for predicting increased disease risk, systemic inflammation, disease severity, and poor prognosis of sepsis via interacting with miR‐21

Lnc‐MEG3 acts as a potential biomarker for predicting increased disease risk, systemic inflammation, disease severity, and poor prognosis of sepsis via interacting with miR‐21

Long non-coding RNA Gm12664 up-regulates CAV1 expression to promote hepatocellular lipid accumulation by sponging miR-295-5p

Lnc‐hipk1 inhibits mouse adipocyte apoptosis as a sponge of miR‐497

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