LncRNA MAGI2-AS3 inhibited tumour progression by up-regulating STAM via interacting with miR-142-3p in clear cell renal cell carcinoma

Abstract

Background: Non-coding RNA (ncRNA) is a class of RNAs that do not encode proteins but have multiple biological functions intracellular. They play an indispensable role in the occurrence and development of tumour. Therefore, understanding their molecular regulatory mechanisms in tumour cells are important for the treatment of tumour patients. Methods: The upstream ncRNAs of STAM were predicted by bioinformatic analysis, and the specific mechanism of lncRNA MAGI2-AS3 regulated miR-142-3p affecting STAM expression and participating in ccRCC cell proliferation, invasion, migration and apoptosis was detected by RT-qPCR, Western blotting, cellular functional experiments and luciferase reporter assay. Results: Overexpression of miR-142-3p or silencing of MAGI2-AS3 promoted the proliferation and migration of ccRCC cells, and inhibits cell apoptosis, while silencing of miR-142-3p or overexpression of MAGI2-AS3 had the opposite effect on ccRCC cells. Furthermore, we confirmed that MAGI2-AS3 acted as sponge and combined with miR-142-3p, blocked the inhibitory effect of miR-142-3p on the expression of STAM, thereby involved in ccRCC proliferation and metastasis. Furthermore, the prognostic model based on the MAGI2-AS3/miR-142-3p/STAM axis further emphasizes its prognostic value in ccRCC. Conclusion: MAGI2-AS3 competitively binding miR-142-3p to upregulate the STAM expression suppressing ccRCC proliferation and metastasis. MAGI2-AS3/miR-142-3p/STAM axis may serve as a promising ccRCC therapeutic target.