LncRNA LOC285194 modulates gastric carcinoma progression through activating Wnt/β‐catenin signaling pathway

Abstract

Emerging evidences have revealed long noncoding RNAs (lncRNAs’) critical roles in diverse human carcinoma. Among these cancers, lncRNA LOC285194 has been extensively investigated in several types of carcinomas in the recent years. Nevertheless, the biological function, clinical relevance, and the influence of LOC285194 in gastric cancer (GC) are not fully understood. The present study aims to explore the biological function of LOC285194 in the progression and development of GC. First, LOC285194 expressions were detected in GC tissues and cell lines. The functional role of LOC285194 in GC was evaluated both in vitro and in vivo. Our data found that LOC285194 was lowly expressed both in human GC tissues and GC cell lines compared with corresponding normal controls. Moreover, LOC285194 was mitigated by transfection with LV‐LOC285194 in both HGC‐27 and MKN45 cell lines. Silencing of LOC285194 remarkably induced GC cell livability and cell proliferation. On the contrary, the LOC285194 overexpression suppressed MKN45 and HGC‐27 cell proliferation and promoted cell apoptosis. Additionally, silencing of LOC285194 increased the ability of colony formation, cell migration, and invasive capacities, together with blocking the apoptotic rates of GC cells. Correspondently, LOC285194 overexpression exerted the opposite effects. Mechanistically, silencing of LOC285194 promoted GC progression via inducing Wnt signaling activity. Moreover, in vivo xenografts nude mice model results showed that LOC285194 inhibited GC progression through targeting Wnt signaling. Taken together, LOC285194 is associated with GC progression by regulating the Wnt signaling transduction, potentiating LOC285194's promising role as a novel treatment biomarker in GC.

RETRACTED ARTICLE: Propofol inhibits migration and induces apoptosis of pancreatic cancer PANC-1 cells through miR-34a-mediated E-cadherin and LOC285194 signals

“…Experimental research found that LOC285194 overexpression inhibited cell proliferation and induced apoptosis in vascular smooth muscle cells (VSMCs) in vitro, and vice versa [23]. In gastric cancer MKN45 and HGC-27 cells in vitro and in vivo, LOC285194 overexpression suppressed cell proliferation and promoted cell apoptosis [24].…”

BASP1 Suppresses Cell Growth and Metastasis through Inhibiting Wnt/β-Catenin Pathway in Gastric Cancer

“…Chen et al [ 26 ] have reported that TYRO3 promotes cell growth and metastasis through activating the Wnt/ β -catenin pathway in GC. Zhong et al [ 27 ] have suggested that LOC285194 could facilitate GC progression through the activation of the Wnt/ β -catenin pathway. In the present study, we explored the effect of BASP1 on the expression of WT1, Wnt, and β -catenin in GC cells by qRT-PCR and western blot assays.…”

Long non-coding RNA LOC285194 inhibits proliferation and migration but promoted apoptosis in vascular smooth muscle cells via targeting miR-211/PUMA and TGF-β1/S100A4 signal

“…Identifying the mechanisms that regulate VSMC apoptosis potentially may lead to novel therapeutic approaches that are more efficacious for these patients. Previous works showed that LOC285194 overexpression induced cell apoptosis in gastric carcinoma [ 28 ], pancreatic cancer [ 29 ] and colon cancer [ 10 ]. In the present study, we demonstrated that LOC285194 overexpression significantly induced A7r5 cell apoptosis.…”