LncRNA LINC01871 sponging miR-142-3p to modulate ZYG11B promotes the chemoresistance of colorectal cancer cells by inducing autophagy

Abstract

Background Colorectal cancer (CRC) is a malignant tumor in the digestive tract. Increasing evidence indicated that chemoresistance leads to a poor prognosis of CRC. Herein, we aimed to uncover the potential mechanism by which long intergenic noncoding RNA-1871 (LINC01871) affects the chemoresistance of CRC cells. Methods Relative level of LINC01871 in CRC tissues was assessed by reverse transcription quantitative PCR (RT-qPCR). Kaplan–Meier analysis was conducted to determine the relevance of LINC01871 and the prognosis of CRC patients. Cell Counting Kit-8 (CCK-8) and colony formation assay were used to evaluate the proliferation of SW480 cells. Expression levels of proteins and their genes were assessed by western blot, immunofluorescence staining and RT-qPCR. In addition, the interaction of LINC01871, miR-142-3p and protein zyg-11 homolog B (ZYG11B) were analyzed via dual-luciferase reporter assays. Results LINC01871 was low-expressed in CRC tissues and cell lines. Patients with a low level of LINC01871 showed significantly lower survival rate. pcDNA-LINC01871 significantly reduced the viability of SW480 cells (P < 0.01), elevated SW480 cells sensitivity to 5-FU (P < 0.01), reduced LC3 punctate aggregates (P < 0.01) and downregulated the relative mRNA expression level of autophagy related protein 9A, autophagy related protein 4B and high mobility group box 1 (P < 0.01) in SW480 cells. Moreover, LINC01871 was found to sponge miR-142-3p, and ZYG11B was the target of miR-142-3p. MiR-142-3p mimic significantly recovered the effect of pcDNA-LINC001871, whereas pcDNA-ZYG11B reversed the recovery effect of the miR-142-3p mimic. Conclusion LINC01871/miR-142-3p/ ZYG11B axis regulates the chemoresistance of CRCs by inducing autophagy.

The role of LncRNA-mediated autophagy in cancer progression

“…LINC01871 was found to be expressed at low levels in CRC tissues and cell lines, and patients with low LINC01871 levels had significantly worse survival. LINC01871 can sponge miR-142-3p and regulate ZYG11B expression to induce autophagy, which increases CRC cell sensitivity to 5-FU and promotes CRC cell chemotherapy resistance ( Duan et al, 2023 ). Similarly, knockdown of lncRNA NEAT1 significantly inhibited CRC cell proliferation, autophagy and enhanced 5-FU sensitivity via targeting miR-34a ( Liu et al, 2020 ).…”