LncRNA LINC01339 Hinders the Development of Wilms’ Tumor via MiR-135b-3p/ADH1C Axis

Abstract

Wilms’ tumor is a malignant renal cancer that arises within the pediatric urinary system. This study intended to investigate how a novel long non-coding RNA LINC01339 functions in the pathogenesis of Wilms’ tumor. An elevated miR-135b-3p expression as well as reduced levels of LINC01339 and ADH1C were observed in Wilms’ tumor. LINC01339 mediated ADH1C expression by directly binding to miR-135b-3p. The enforced LINC01339 or ADH1C markedly hindered cell growth and migration in Wilms’ tumor. The LINC01339 overexpression also repressed the growth of Wilms’ tumors in vivo, whereas miR-135b-3p overexpression exerted the opposite effects on Wilms’ tumor cells in vitro. Additionally, upregulating miR-135b-3p reversed LINC01339’s effects on the cellular processes of Wilms’ tumor cells, whereas ADH1C overexpression offset the cancer-promoting influence of miR-135b-3p upregulation on Wilms’ tumor progression. Therefore, LINC01339 prevents Wilms’ tumor progression by modulating the miR-135b-3p/ADH1C axis. Our findings substantiate that the LINC01339/miR-135 b-3p/ADH1C regulatory axis has potential to be a target for the treatment of Wilms’ tumor.