LncRNA KCNQ1OT1 regulates proliferation and cisplatin resistance in tongue cancer via miR-211-5p mediated Ezrin/Fak/Src signaling

Zhang, Shanyi; Ma, Hanyu; Zhang, Daming; Xie, Shuhua; Wang, Weiwei; Li, Qunxing; Lin, Zhaoyu; Wang, Youyuan

doi:10.1038/s41419-018-0793-5

Cited by 189 publications

(174 citation statements)

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“…For example, KCNQ1OT1 promotes the progression of non–small cell lung cancer (NSCLC) through upregulating HSP90AA1 by sponging miR‐27b . In tongue cancer, it also has been reported KCNQ1OT1 directly interacts with miR‐211‐5p to facilitate cell proliferation and cisplatin resistance . Therefore, we hypothesized that the function of KCNQ1OT1 in MSSCC was also in part mediated by miRNAs.…”

Section: Discussionmentioning

confidence: 99%

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Long noncoding RNA KCNQ1OT1 promotes proliferation, migration, and invasion in maxillary sinus squamous cell carcinoma by regulating miR‐204/EphA7 axis

Sun

et al. 2019

J of Cellular Biochemistry

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Long noncoding RNAs have been demonstrated to contribute to the development and progression of various cancers. However, the underlying regulatory mechanisms of KCNQ1OT1 in tumorigenesis of maxillary sinus squamous cell carcinoma (MSSCC) remain unknown. Herein, we found that KCNQ1OT1 expression was markedly upregulated in MSSCC tissues and MSSCC cell line (IMC‐3) by using quantitative reverse transcription‐polymerase chain reaction. Loss‐of‐function experiments revealed that the deletion of KCNQ1OT1 inhibited cell proliferation, migration, and invasion. Moreover, we confirmed KCNQ1OT1 could directly interact with miR‐204 by bioinformatic prediction and dual luciferase assay, and miR‐204 inhibitor markedly reversed MSSCC tumor phenotypes induced by shKCNQ1OT1. Finally, we demonstrated that KCNQ1OT1/miR‐204 facilitated MSSCC progression by regulating Eph receptor A7 (EphA7). Taken together, these results revealed a novel regulatory mechanism KCNQ1OT1/miR‐204/EphA7 axis, which could provide a new understanding of MSSCC tumorigenesis and develop potential targets for MSSCC therapy.

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Section: Discussionmentioning

confidence: 99%

“…32 In tongue cancer, it also has been reported KCNQ1OT1 directly interacts with miR-211-5p to facilitate cell proliferation and cisplatin resistance. 33 Therefore, we hypothesized that the function of KCNQ1OT1 in MSSCC was also in part mediated by miRNAs. Consistently, we found that KCNQ1OT1 could directly bind miR-204.…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA KCNQ1OT1 promotes proliferation, migration, and invasion in maxillary sinus squamous cell carcinoma by regulating miR‐204/EphA7 axis

Sun

et al. 2019

J of Cellular Biochemistry

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“…After 48 h transfection, CAL27 and SCC25 cells (2 × 10 4 / well) were seeded in 24-well plates. The 5-ethynyl-2′deoxyuridine (EdU) assay (Life Technologies Corporation, USA) was used to evaluate the proliferation ability of OSCC cells as previously reported [16]. Briefly, CAL27 and SCC25 cells were incubated with 100 μL EdU reagent for 2 h at 37°C, and stained with DAPI and visualized by a fluorescence microscope (Olympus, Tokyo, Japan).…”

Section: Edu Assaymentioning

confidence: 99%

Linc01234 promotes cell proliferation and metastasis in oral squamous cell carcinoma via miR-433/PAK4 axis

Liu

Jian

et al. 2020

BMC Cancer

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Background: Increasing studies have demonstrated that long non-coding RNAs (lncRNAs) play an important role in tumor progression. However, the potential biological functions and clinical importance of Linc01234 in oral squamous cell carcinoma (OSCC) remain unclear. Methods: We evaluated the expression profile and prognostic value of Linc01234 in OSCC tissues by RT-qPCR. Then, functional in vitro experiments were performed to investigate the effects of Linc01234 on tumor growth, migration and invasion in OSCC. Mechanistically, RT-qPCR, bioinformatic analysis and dual luciferase reporter assays were performed to identify a competitive endogenous RNA (ceRNA) mechanism involving Linc01234, miR-433-3p and PAK4. Results: We found that Linc01234 was clearly upregulated in OSCC tissues and cell lines, and its level was positively associated with T stage, lymph node metastasis, differentiation and poor prognosis of patients with OSCC. Our results shown that Linc01234 inhibited cell proliferation and metastatic abilities in CAL27 and SCC25 cells following its knockdown. Mechanistic analysis indicated that Linc01234 may act as a ceRNA (competing endogenous RNA) of miR-433-3p to relieve the repressive effect of miR-433-3p on its target PAK4. Conclusions:Our results indicated that Linc01234 promotes OSCC progression through the Linc01234/miR-433/ PAK4 axis and might be a potential therapeutic target for OSCC.

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“…Cyclum performed the best in this analysis (Figure 4a and Additional file 1, Table S2), even on samples that reportedly had few cycling cells (e.g., Mel79). Among the novel cell-cycling genes nominated by Cyclum (Additional file 1, Table S2, Figure S3, and S4), KCNQ1OT1 and FBLIM1 (Additional file 1, Figure S3, and Table S3) have recently been shown in the literature to be related to proliferation and tumorigenesis [21][22][23][24][25][26].…”

Section: Application Of Cyclum On the Melanoma Datamentioning

confidence: 99%

Latent periodic process inference from single-cell RNA-seq data

Liang

Wang

Han

et al. 2019

Preprint

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Convoluted biological processes underlie the development of multicellular organisms and diseases. Advances in scRNA-seq make it possible to study these processes from cells at various developmental stages. Achieving accurate characterization is challenging, however, particularly for periodic processes, such as cell cycles. To address this, we developed Cyclum, a novel AutoEncoder approach that characterizes circular trajectories in the high-dimensional gene expression space. Cyclum substantially improves the accuracy and robustness of cell-cycle characterization beyond existing approaches. Applying Cyclum to removing cell-cycle effects leads to substantially improved delineations of cell subpopulations, which is useful for establishing various cell atlases and studying tumor heterogeneity. Cyclum is available at https://github.com/KChen-lab/cyclum. BackgroundConvoluted biological processes, which involve cell proliferation, differentiation, state transition, and cell-to-cell communication [1,2]. The course of development can be influenced by genetic (e.g., mutations), epigenetic, and environmental factors. Alterations to the genome, transcriptome, and proteome of individual cells also can result in pathogeneses [3]. Early efforts have been made to reconstruct the temporal ordering of biological samples using bulk data [4,5], although challenges associated with cellular heterogeneity make it difficult to infer accurate time series. Advances in single-cell RNA sequencing (scRNA-seq) enabled large-scale acquisition of singlecell transcriptomic profiles and provided an unprecedented opportunity to uncover latent biological processes that orchestrate dynamic expression of genes in single cells throughout the course of the development [6]. However, it is very challenging to deconvolute these processes from scRNA-seq data accurately. A sufficiently large number of cells across time, lineage, and space need to be sampled in order to capture detailed sub-populational features and reduce technological noise. Tremendous efforts have been made to develop trajectory inference methods from scRNA-seq data. Over 59 methods have been developed since 2014 [7], including the widely known Monocle and Wanderlust. These methods represent biological processes in linear, bifurcating, or other graph topologies.In many developmental processes, such as embryogenesis, organogenesis, and tumorigenesis [8], cell cycle plays a fundamental role. Distinct from processes that evoke linear changes in gene expression, cell cycle causes periodicity. A cycle starts from the G1 phase, goes through S and G2/M, and then returns to G1 within 24-hours for human cells [2]. This process is orchestrated elegantly by variable sets of genes (e.g., cyclins and cyclin-dependent kinases) that are turned on and off at relatively precise timings. As a result of such periodicity, the cycling cells at different transcriptomic states form a circular, non-linear trajectory in high-dimensional gene expression spaces. The positions of a cell alongside the circular trajec...

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LncRNA KCNQ1OT1 regulates proliferation and cisplatin resistance in tongue cancer via miR-211-5p mediated Ezrin/Fak/Src signaling

Cited by 189 publications

References 47 publications

Long noncoding RNA KCNQ1OT1 promotes proliferation, migration, and invasion in maxillary sinus squamous cell carcinoma by regulating miR‐204/EphA7 axis

Long noncoding RNA KCNQ1OT1 promotes proliferation, migration, and invasion in maxillary sinus squamous cell carcinoma by regulating miR‐204/EphA7 axis

Linc01234 promotes cell proliferation and metastasis in oral squamous cell carcinoma via miR-433/PAK4 axis

Latent periodic process inference from single-cell RNA-seq data

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