LncRNA HULC promotes lung squamous cell carcinoma by regulating PTPRO via NF‐κB

Xu, Yuping; Li, Jingyun; Wang, Pingping; Zhang, Zhenliang; Wang, Xuetao

doi:10.1002/jcb.29119

Cited by 34 publications

(26 citation statements)

References 42 publications

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“…They have been extensively studied in the carcinogenesis of many diseases, including cancer. Moreover, recent findings manifested that lncR-NAs are involved in a series of biological processes, such as cell proliferation, apoptosis, invasion and migration [9,10]. Furthermore, lncRNAs have been identified as new biomarkers of many malignancies, since they are associated with the complicated pathogenesis of malignancies [11,12].…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA RHPN1-AS1 promotes colorectal cancer progression via targeting miR-7-5p/OGT axis

Zheng

Zhang

et al. 2020

Cancer Cell Int

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Background: Rhophilin Rho GTPase binding protein 1 antisense RNA 1 (RHPN1-AS1) is a newly discovered oncogene in several diseases, such as breast cancer, non-small cell lung cancer and uveal melanoma. Nevertheless, its molecular role in colorectal cancer (CRC) remains unknown. This paper explored the role of RHPN1-AS1 in CRC progression.Methods: qRT-PCR was used to detect relevant RNAs expression. CCK-8, EdU, flow cytometry, Transwell and western blot assays were performed to investigate the function of RHPN1-AS1 in CRC cells. Xenograft model was constructed to evaluate the effects of RHPN1-AS1 on tumor growth in vivo. Mechanical experiments were performed to investigate the relationship between relative genes.Results: RHPN1-AS1 was significantly overexpressed in CRC cell lines. Knockdown of RHPN1-AS1 could inhibit cell proliferation, while stimulating cell apoptosis in vitro. Cell migration and invasion abilities were greatly suppressed after silencing RHPN1-AS1. Besides, signal transducer and activator of transcription 3 (STAT3) served as transcription factor of RHPN1-AS1. Moreover, miR-7-5p was identified as a target of RHPN1-AS1 and was negatively regulated by RHPN1-AS1 in CRC. MiR-7-5p inhibition rescued the oncogenic function of RHPN1-AS1. Additionally, O-GlcNAcylation transferase (OGT) was the downstream target of miR-7-5p. OGT overexpression could abrogate the anti-tumor effects of RHPN1-AS1 knockdown on CRC.Conclusion: RHPN1-AS1 regulates CRC by mediating OGT through sponging miR-7-5p, suggesting that RHPN1-AS1 might be a potential therapeutic target for CRC.

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Section: Introductionmentioning

confidence: 99%

Long noncoding RNA RHPN1-AS1 promotes colorectal cancer progression via targeting miR-7-5p/OGT axis

Zheng

Zhang

et al. 2020

Cancer Cell Int

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“…5,6 In lung squamous cell carcinoma, lncRNA HULC accelerates tumor progression by changing the expression of PTPRO through regulating nuclear factor-κB (NF-κB). 7 In breast cancer, lncRNA NONHSAT101069 regulates the miR-129-5p/ Twist1, promoting epirubicin resistance, migration, and invasion. 8 In osteosarcoma, lncRNA AFAP1-AS1 acts as an oncogene to promote the development and epithelialmesenchymal transition (EMT) via RhoC/ROCK1/ p38MAPK/Twist1 signaling axis.…”

Section: Introductionmentioning

confidence: 99%

Up-regulated long non-coding RNA ILF3-AS1 indicates poor prognosis of nasopharyngeal carcinoma and promoted cell metastasis

2020

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Background: Long non-coding RNAs (lncRNAs) have been confirmed to participate in the regulation of nasopharyngeal carcinoma. Here, we endeavored to explore the character of lncRNA ILF3-AS1 in the nasopharyngeal carcinoma and its function. Methods: A total of 68 nasopharyngeal carcinoma tissues and adjacent normal nasopharyngeal tissues were collected. Expressions of lncRNA ILF3-AS1 in these tissues were detected using quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between the expression level of lncRNA ILF3-AS1 and clinical pathological characteristics was analyzed. Inhibition of lncRNA ILF3-AS1 was done using small interference RNA. Results: lncRNA ILF3-AS1 expression was significantly up-regulated in the 68 nasopharyngeal carcinoma tissue samples compared to their adjacent normal tissue samples. Increased lncRNA ILF3-AS1 level was related to the advanced tumor node metastasis stage and the metastasis of nasopharyngeal carcinoma. Also, increased lncRNA ILF3-AS1 indicated poor prognosis of nasopharyngeal carcinoma patients. Inhibition of lncRNA ILF3-AS1 reduced proliferation, invasion and migration of nasopharyngeal carcinoma cells. MicroRNA-320a (miR-320a) was determined as a direct target for lncRNA ILF3-AS1 in nasopharyngeal carcinoma. Furthermore, lncRNA ILF3-AS1 could sponge miR-320a to promote BMI1 expression. The expression of BMI1 was significantly inhibited by the down-regulation of lncRNA ILF3-AS1. Conclusions: For the first time, we demonstrated that lncRNA ILF3-AS1 was markedly over-expressed in nasopharyngeal carcinoma tissues and cells. Elevated lncRNA ILF3-AS1 expression was correlated with severe cancer stage and poor prognosis. lncRNA ILF3-AS1 could promote proliferation, invasion, and migration of cells, which might indicate a novel target site for the future diagnosis and therapy of nasopharyngeal carcinoma.

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“…Huang et al [24] found that increasing the expression of the downregulated lncRNA LINC00961 resulted in increased Bax expression and the corresponding apoptotic death of NSCLC cells. Xu et al further provided evidence suggesting that the lncRNA HULC is able to promote LSCC cell proliferation owing to its ability to PTPROdependent phosphorylation and activation of NF-κB [25]. Similarly, Wang et al found that increased expression of the lncRNA MIR31HG in NSCLC led to enhanced tumor cell ge tinib resistance owing to associated activation of the EGFR/PI3K/AKT signaling pathway.…”

Section: Discussionmentioning

confidence: 98%

Identification of a prognostic three-long noncoding RNA signature in lung squamous cell carcinoma via bioinformatic analysis

Zheng

Wang

et al. 2020

Preprint

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Background Lung squamous cell carcinoma (LSCC) is a form of cancer that is associated with high rates of relapse, poor responsiveness to therapy, and a relatively poor prognosis. The relationship between long non-coding RNA (lncRNA) expression and LSCC patient prognosis remains to be established. Methods In the present study, we discovered that lncRNAs were differentially expressed in LSCC tumor tissues relative to normal control tissues, and we explored the prognostic relevance of these lncRNA expression patterns using data from the Cancer Genome Atlas (TCGA). Results These multidimensional data were analyzed in order to identify lncRNA signatures that were associated with LSCC patient survival outcomes. Kaplan-Meier survival curves revealed prognostic capabilities for three of these lncRNAs (LINC02555, APCDD1L-DT and OTX2-AS1). A Cox regression analysis revealed this three-lncRNA signature to be significantly associated with patient survival. Further GO and KEGG analyses revealed that the predicted target genes of these three lncRNAs were also potentially involved in cancer-associated pathways. Conclusions Together these results thus indicate that this novel three-lncRNA signature can be used to predict LSCC patient prognosis.

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LncRNA HULC promotes lung squamous cell carcinoma by regulating PTPRO via NF‐κB

Cited by 34 publications

References 42 publications

Long noncoding RNA RHPN1-AS1 promotes colorectal cancer progression via targeting miR-7-5p/OGT axis

Long noncoding RNA RHPN1-AS1 promotes colorectal cancer progression via targeting miR-7-5p/OGT axis

Up-regulated long non-coding RNA ILF3-AS1 indicates poor prognosis of nasopharyngeal carcinoma and promoted cell metastasis

Identification of a prognostic three-long noncoding RNA signature in lung squamous cell carcinoma via bioinformatic analysis

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