LncRNA HCP5 enhances the proliferation and migration of cervical cancer via miR-216a-5p/CDC42 axis

Abstract

To investigate the important roles of the cancer-promoting long non-coding RNAs (lncRNAs) in cervical cancer, the up-regulated lncRNAs and prognostic analysis were identified through Lnc2Cancer and Lncar. LncRNA-regulated miRNA and miRNA-target mRNA were analyzed based on starBase v2.0 and miTarbase to predict the lncRNA-miRNA-mRNA ceRNA network. Based on the above findings, the abnormally expressed histocompatibility leukocyte antigen complex P5 (HCP5) was identified in 31 cervical cancer patients through RT-qPCR. The stable cell lines were constructed to explore the effect of HCP5 on the promotion of cervical cancer and the regulatory role on the expression of miR-216a-5p and CDC42. Cell Counting Kit-8 (CCK8) assay, cell clone formation, and transwell assay were used to examine proliferation and migration ability of cervical cancer cells. The results displayed that the overexpression of HCP5 promoted cervical cancer cell proliferation and migration in vitro, and the elevated HCP5 can also promote tumor growth in vivo. Besides, RT-qPCR and western blot assay revealed that elevated HCP5 suppressed miR-216a-5p expression and then up-regulated the expression of CDC42. In contrast, knocking down HCP5 resulted in increased expression of miR-216a-5p and then downregulated the expression of CDC42. Rescue experiments also demonstrated that miR-216a-5p could in part intercept in promotion impact caused by HCP5 on cervical cancer cells. Above all, HCP5, as an oncogene, can promote proliferation and migration ability of cervical cancer via the regulation of the miR-216a-5p/CDC42 axis.

Downregulation of long noncoding RNA HCP5/miR-216a-5p/ZEB1 axis inhibits the malignant biological function of laryngeal squamous cell carcinoma cells

“…HCP5 promotes cancer development by sponging miR-140-5p, miR −138−5p, miR-29b-3p, and miR-143-3p (21)(22)(23)(24). In addition, HCP5 can sponge miR-216a-5p in cervical cancer (34). Here, we confirm that HCP5 promotes LSCC progression via sponging miR-216a-5p.…”

Molecular mechanisms of microRNA-216a during tumor progression

“…CDC42 is a component of the Rho GTPase family and is involved in cell proliferation and migration [ 172 , 173 ]. HCP5 promoted the cervical tumor initiation and progression via the miR-216a-5p/CDC42 axis [ 174 ].…”

MALAT1 promotes FOXA1 degradation by competitively binding to miR-216a-5p and enhancing neuroendocrine differentiation in prostate cancer

“…A growing number of studies support a broad regulatory network within competing endogenous RNAs (ceRNAs), suggesting that non-coding RNA (ncRNA) sharing microRNAs (miRNAs/miRs) binding sites can regulate target mRNAs through competitive post-transcriptional control [ 19 , 20 ]. miR-216a-5p can inhibit the growth of various cancers via a ceRNA mechanism modulated by lncRNAs [21] , [22] , [23] . More importantly, putative binding sites of miR-216a-5p to zinc finger protein 91 (ZFP91) have been identified by bioinformatics prediction.…”