LncRNA H19 inhibits oxidative stress injury of cochlear hair cells by regulating miR-653-5p/SIRT1 axis

Xie, Weibin; Shu, Ting; Peng, Haisen; Liu, Jiali; Li, Chunhua; Wang, Meiqun; Wu, Ping; Liu, Yuehui

doi:10.3724/abbs.2022018

Cited by 7 publications

(7 citation statements)

References 27 publications

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“…Using TaqMan array Advanced miRNA (Thermo Fisher Scientific, Waltham, MA, USA), the differential expression of miRNAs in the SH-SY5Y cells from [1 μΜ]-exposed of EDCs and VH were profiled (data not shown). The candidate miRNAs were then selected owing to their reported or predicted role in neuronal proliferation and survival [ 30 , 31 , 32 , 33 , 34 ]. Among them, miR-18b-5p, miR-29b-3p, miR-146b-5p, miR-452-5p, and miR-653-5p were carefully chosen for qRT-PCR validation both in the undifferentiated and differentiated SH-SY5Y cells treated with ATZ, CYP, and VNZ [100 nM, 1 μΜ] for 48 h. The expression levels of miR-18b-5p, miR-29b-3p, miR-146b-5p, miR-452-5p and miR-653-5p were significantly downregulated by EDC treatment compared with the VH ( Table 1 ) in the differentiated SH-SY5Y cells.…”

Section: Resultsmentioning

confidence: 99%

Effects of Subtoxic Concentrations of Atrazine, Cypermethrin, and Vinclozolin on microRNA-Mediated PI3K/Akt/mTOR Signaling in SH-SY5Y Cells

Graziosi

Sita

Corrieri

et al. 2022

IJMS

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Endocrine-disrupting chemicals (EDCs) are different natural and synthetic chemicals that may interfere with several mechanisms of the endocrine system producing adverse developmental, metabolic, reproductive, and neurological effects in both human beings and wildlife. Among pesticides, numerous chemicals have been identified as EDCs. MicroRNAs (miRNAs) can regulate gene expression, making fine adjustments in mRNA abundance and regulating proteostasis. We hypothesized that exposure to low doses of atrazine, cypermethrin, and vinclozolin may lead to effects on miRNA expression in SH-SY5Y cells. In particular, the exposure of SH-SY5Y cells to subtoxic concentrations of vinclozolin is able to downregulate miR-29b-3p expression leading to the increase in the related gene expression of ADAM12 and CDK6, which may promote a pro-oncogenic response through the activation of the PI3K/Akt/mTOR pathway and counteracting p53 activity. A better understanding of the molecular mechanisms of EDCs could provide important insight into their role in human disease.

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Section: Resultsmentioning

confidence: 99%

Effects of Subtoxic Concentrations of Atrazine, Cypermethrin, and Vinclozolin on microRNA-Mediated PI3K/Akt/mTOR Signaling in SH-SY5Y Cells

Graziosi

Sita

Corrieri

et al. 2022

IJMS

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“…Oxidative stress injury associated with increasing age was a critical mechanism of age-related hearing loss, and several lncRNAs had been found to act in regulating oxidative stress in cells. LncRNA H19 was found to be notably down-regulated in the cochlea of aged mice, and could inhibit the oxidative stress injury in cochlear hair cells through miR-653-5p/SIRT1 axis ( Xie et al, 2022 ). Li et al (2022) demonstrated that lncRNA Gm44593 could regulate the miR-29b/WNK axis to improve oxidative stress injury in HEI-OC1 cells.…”

Section: Lncrna In Hearing Lossmentioning

confidence: 99%

“…Regulate apoptosis, autophagy and mitochondrial function Miao et al, 2021;Michels et al, 2019;Mick et al, 2014;Mittal et al, 2017;Mu et al, 2023;Nieman and Oh, 2020;Nunez et al, 2020 miR-29b Weston et al, 2006 the cochlea of aged mice, and could inhibit the oxidative stress injury in cochlear hair cells through miR-653-5p/SIRT1 axis (Xie et al, 2022). Li et al (2022) demonstrated that lncRNA Gm44593 could regulate the miR-29b/WNK axis to improve oxidative stress injury in HEI-OC1 cells.…”

Section: Lncrna In Hearing Lossmentioning

confidence: 99%

NcRNA: key and potential in hearing loss

Zhu,

Wang,

et al. 2024

Front. Neurosci.

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Hearing loss has an extremely high prevalence worldwide and brings incredible economic and social burdens. Mechanisms such as epigenetics are profoundly involved in the initiation and progression of hearing loss and potentially yield definite strategies for hearing loss treatment. Non-coding genes occupy 97% of the human genome, and their transcripts, non-coding RNAs (ncRNAs), are widely participated in regulating various physiological and pathological situations. NcRNAs, mainly including micro-RNAs (miRNAs), long-stranded non-coding RNAs (lncRNAs), and circular RNAs (circRNAs), are involved in the regulation of cell metabolism and cell death by modulating gene expression and protein-protein interactions, thus impacting the occurrence and prognosis of hearing loss. This review provides a detailed overview of ncRNAs, especially miRNAs and lncRNAs, in the pathogenesis of hearing loss. We also discuss the shortcomings and issues that need to be addressed in the study of hearing loss ncRNAs in the hope of providing viable therapeutic strategies for the precise treatment of hearing loss.

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“…In H 2 O 2 -stimulated HEI-OC1 cells, overexpression of H19 reduces mitochondrial ROS production and the apoptosis ratio. The mechanism is that lncRNA H19 protects cochlear hair cells from OS via the miR-653-5p/SIRT1 axis [ 16 ]. However, few lncRNAs induced by OS in the jejunum of piglets have been reported.…”

Section: Introductionmentioning

confidence: 99%

Identification and functional prediction of long non-coding RNAs related to oxidative stress in the jejunum of piglets

Li,

Zhang,

Jin

et al. 2024

Anim Biosci

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Objective: Oxidative stress (OS) is a pathological process arising from the excessive production of free radicals in the body. It has the potential to alter animal gene expression and cause damage to the jejunum. However, there have been few reports of changes in the expression of long noncoding RNAs (lncRNAs) in the jejunum in piglets under OS. The purpose of this research was to examine how lncRNAs in piglet jejunum change under OS.Methods: The abdominal cavities of piglets were injected with diquat (DQ) to produce OS. Raw reads were downloaded from the SRA database. RNA-seq was utilized to study the expression of lncRNAs in piglets under OS. Additionally, six randomly selected lncRNAs were verified using quantitative real-time polymerase chain reaction (qRT‒PCR) to examine the mechanism of oxidative damage.Results: A total of 79 lncRNAs were differentially expressed (DE) in the treatment group compared to the negative control group. The target genes of DE lncRNAs were enriched in gene ontology (GO) terms and Kyoto encyclopedia of genes and genomes (KEGG) signaling pathways. Chemical carcinogenesis-reactive oxygen species, the Foxo signaling pathway, colorectal cancer, and the AMPK signaling pathway were all linked to OS.Conclusion: Our results demonstrated that DQ-induced OS causes differential expression of lncRNAs, laying the groundwork for future research into the processes involved in the jejunum’s response to OS.

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LncRNA H19 inhibits oxidative stress injury of cochlear hair cells by regulating miR-653-5p/SIRT1 axis

Cited by 7 publications

References 27 publications

Effects of Subtoxic Concentrations of Atrazine, Cypermethrin, and Vinclozolin on microRNA-Mediated PI3K/Akt/mTOR Signaling in SH-SY5Y Cells

Effects of Subtoxic Concentrations of Atrazine, Cypermethrin, and Vinclozolin on microRNA-Mediated PI3K/Akt/mTOR Signaling in SH-SY5Y Cells

NcRNA: key and potential in hearing loss

Identification and functional prediction of long non-coding RNAs related to oxidative stress in the jejunum of piglets

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