LncRNA GAS6-AS2 promotes non-small-cell lung cancer cell proliferation via regulating miR-144-3p/ MAPK6 axis

Hou, Guangjie; Yang, Junfeng; Tang, Jinxing; He, Yi

doi:10.1080/15384101.2020.1867782

Cited by 11 publications

(10 citation statements)

References 28 publications

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“…3b, c). Of the increased miRNAs, miRLet-7f-5p [44], miRLet-7e-5p [45], miRLet-7c-5p [46], miR29a-3p [47], miR146b-5p [48], miRLet-7d-5p [49], miR338-3p [50], miR144-3p [51], miRLet-7j [52], miR449a-5p [53], miR30c-1-3p [54], miR147-3p [55], miR30c-2-3p [56], and miR1195 [57] have been attributed to a range of biological processes including cellular proliferation, cellular differentiation, and cellular signaling and communication. The miRNAs miR3074-1-3p, miR3095-3p, miR344b-3p, and miR3057-5p have no reported biological functions.…”

Section: Resultsmentioning

confidence: 99%

Characterization of the microRNA Transcriptomes and Proteomics of Cochlea Tissue-derived Small Extracellular Vesicles From Different Age of Mice After Birth

Jiang¹,

Ma²,

Han³

et al. 2021

Preprint

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The cochlea is an important sensory organ for both balance and sound perception, and the formation of the cochlea is a complex developmental process. The development of the mouse cochlea begins on embryonic day (E)9 and continues until postnatal day (P)21 when the hearing system is considered mature. Small extracellular vesicles (sEVs), with a diameter ranging from 30 nm to 200 nm, have been considered as a significant medium for information communication in both the processing of physiological and pathological. However, there are no studies exploring the role of sEVs in the development of the cochlea. Here, we isolated tissue-derived sEVs from the cochleae of FVB mice at P3, P7, P14, and P21 by ultracentrifugation. These sEVs were first characterized by transmission electron microscopy, nanoparticle tracking analysis, and western blotting. Next, we used small RNA-seq and mass spectrometry to characterize the microRNA transcriptomes and proteomics of cochlear sEVs from mice at different ages. Many microRNAs and proteins were discovered to be related with inner ear development, anatomical structure development, and the auditory nervous system development. These results all suggest that sEVs exist in the cochlea and are likely to be essential for the normal development of the auditory system. Our findings provide many sEV microRNA and protein targets for future studies of the roles of cochlear sEVs.

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Section: Resultsmentioning

confidence: 99%

Characterization of the microRNA Transcriptomes and Proteomics of Cochlea Tissue-derived Small Extracellular Vesicles From Different Age of Mice After Birth

Jiang¹,

Ma²,

Han³

et al. 2021

Preprint

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“…In this study, the role of GAS6-AS2 in the pathophysiological mechanism of sepsis-related AKI was explored. It has been demonstrated that lncRNA GAS6-AS2 was up-regulated in various cancer cells or tissues [ 9–13 , 26 ]. Here, GAS6-AS2 expression was found to be abnormally up-regulated in HK2 cells treated with LPS and renal tissues from sepsis AKI rats, which was consistent with previous results.…”

Section: Discussionmentioning

confidence: 99%

“…The important role of lncRNAs in sepsis has been well confirmed [ 8 ]. LncRNA GSA6-AS2 is a novel discovered lncRNA, which was mapped to 13q34 with the transcript length of 12,361 bp [ 9 ]. LncRNA GAS6-AS2 has been shown to act as an oncogene to promote the development of a number of tumors, such as hepatocellular carcinoma [ 10 ], breast cancer [ 11 ], osteosarcoma [ 12 ], non-small-cell lung cancer [ 9 ], bladder cancer [ 13 ], and melanoma [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…LncRNA GSA6-AS2 is a novel discovered lncRNA, which was mapped to 13q34 with the transcript length of 12,361 bp [ 9 ]. LncRNA GAS6-AS2 has been shown to act as an oncogene to promote the development of a number of tumors, such as hepatocellular carcinoma [ 10 ], breast cancer [ 11 ], osteosarcoma [ 12 ], non-small-cell lung cancer [ 9 ], bladder cancer [ 13 ], and melanoma [ 14 ]. In addition, existing results indicated that GAS6-AS2 was related to inflammatory response [ 15 ] and apoptosis [ 14 ], which are also the important features of septic AKI.…”

Section: Introductionmentioning

confidence: 99%

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Suppression of lncRNA GAS6-AS2 alleviates sepsis-related acute kidney injury through regulating the miR-136-5p/OXSR1 axis in vitro and in vivo

Cui

Ren

et al. 2022

Renal Failure

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“…Lung cancer is a leading cause of cancer-associated mortality worldwide, with a 5-year survival rate of <15% ( Siegel et al, 2021 ). Proliferation is the leading factor influencing cancer development ( Liu et al, 2021b ; Hou et al, 2021 ; Wang et al, 2021 ). HuR is the key molecule in lung cancer progression ( Liao et al, 2011 ; Li et al, 2013 ; Zhang et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

The Role of RNA-Binding Protein HuR in Lung Cancer by RNA Sequencing Analysis

Xiao

2022

Front. Genet.

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Background: The overexpression of human antigen R (HuR) has been proven in various types of cancer and is associated with the poor survival lung cancer patients. HuR overexpression stabilizes the mRNA of tumor-promoting genes by binding with 3′-UTR AU-rich elements. However, the role of HuR in the proliferation of lung cancer is unclear.Methods: HuR expression was assessed using immunohistochemistry of tumor tissue samples from ten patients with lung cancer and ten patients with benign lung disease. Gene, protein, mRNA, and lncRNA changes in A549 HuR knockdown (KD) cells were assessed by single-cell RNA sequencing analysis. Furthermore, cell proliferation, migration, and invasion were determined by Cell Counting Kit-8 (CCK-8) assays and Transwell assays with or without Matrigel. The cell cycle was assessed by propidium iodide staining. The protein level, mRNA level and half-life of PLK1 were detected by western blotting and RT-qPCR.Results: In clinical patients, the expression of HuR was significantly higher in lung cancer patients than in patients with benign lung disease. RNA sequencing analysis of A549 HuR knockdown cells revealed that the main function of HuR was related to ribonucleoprotein complex biogenesis. HuR was found to regulate signaling pathways mainly related to the spliceosome, RNA transport and the cell cycle. HuR KD suppressed the proliferation, migration and invasion of A549 cells, indicating its promotive role in these processes.Conclusion: These results demonstrate that HuR plays an important role in the progression of lung cancer.

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LncRNA GAS6-AS2 promotes non-small-cell lung cancer cell proliferation via regulating miR-144-3p/ MAPK6 axis

Cited by 11 publications

References 28 publications

Characterization of the microRNA Transcriptomes and Proteomics of Cochlea Tissue-derived Small Extracellular Vesicles From Different Age of Mice After Birth

Characterization of the microRNA Transcriptomes and Proteomics of Cochlea Tissue-derived Small Extracellular Vesicles From Different Age of Mice After Birth

Suppression of lncRNA GAS6-AS2 alleviates sepsis-related acute kidney injury through regulating the miR-136-5p/OXSR1 axis in vitro and in vivo

The Role of RNA-Binding Protein HuR in Lung Cancer by RNA Sequencing Analysis

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