LncRNA FENDRR represses proliferation, migration and invasion through suppression of survivin in cholangiocarcinoma cells

Qin, Xinglei; Lu, Min; Zhou, Yajun; Li, Gang

doi:10.1080/15384101.2019.1598726

Cited by 26 publications

(18 citation statements)

References 27 publications

(30 reference statements)

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“…Fetal-lethal non-coding developmental regulatory RNA (FENDRR) is a new-founded lncRNA which has been reported as a tumor suppressor in different cancers including non-small lung cancer, prostate cancer, breast cancer, cholangiocarcinoma and hepatocellular carcinoma [9][10][11][12]. However, biological effects and its mechanism with respect to CC remain to be explored.…”

Section: Introductionmentioning

confidence: 99%

FENDRR suppresses cervical cancer proliferation and invasion by targeting miR-15a/b-5p and regulating TUBA1A expression

et al. 2020

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Background: Previous literature has revealed long non-coding RNAs (lncRNAs) are crucial regulators for cell functions and gene expression. LncRNA fetal-lethal non-coding developmental regulatory RNA (FENDRR) was reported as a biological suppressor in several types of human cancers, yet relevant mechanisms and biological effects of FENDRR with regards to cervical cancer (CC) are not explored until now. Methods: In this study, quantitative real-time polymerase chain reaction (qRT-PCR) analysis detected gene expression in tissues and cells. Gain-or loss-of-function experiments revealed the biological effects of FENDRR and miR-15a/b-5p on CC cell functions. Bioinformatics tools were used to predict the relevant genes. Mechanism experiments including RNA immunoprecipitation (RIP) assay, pull down assay and luciferase reporter assay depicted the binding situation and coexistence of indicated genes. Results: FENDRR was downregulated in CC tissues and cells, which suppressed CC progression. MiR-15a-5p and miR-15b-5p shared binding sites with FENDRR and had interaction with FENDRR. Tubulin alpha1A (TUBA1A) was downregulated in CC tissues and positively modulated by FENDRR. TUBA1A was the target of miR-15a/b-5p. TUBA1A silencing rescued the effect of FENDRR overexpression on CC cell growth and migration. Conclusion: FENDRR inhibits CC progression through upregulating TUBA1A in a miR-15a/b-5p-dependent manner.

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Section: Introductionmentioning

confidence: 99%

FENDRR suppresses cervical cancer proliferation and invasion by targeting miR-15a/b-5p and regulating TUBA1A expression

et al. 2020

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“…Some lncRNAs could be therapeutic or protective against liver diseases or cancer. For example, expression levels of lncRNA-NEF and FENDRR are downregulated in CCA tissues compared to normal tissues (54,60). Overexpression of these lncRNAs inhibited cell migration and invasion of CCA cell lines HuCCT1, QBC939, or TFK-1 cells, indicating the potentials of lncRNA induction as another therapeutic approach for CCA (54,60).…”

Section: Induction Of Lncrna Expressionmentioning

confidence: 99%

“…FENDRR is a lncRNA, which is downregulated in various cancers such as breast cancer, prostate cancer, and hepatocellular carcinoma (57)(58)(59). A study using 60 CCA patients has found that expression of FENDRR is downregulated in CCA tissues compared to non-cancerous tissues, and FENDRR expression is negatively correlated with expression of survivin (60). Survivin is a protein that inhibits apoptosis and upregulated in cancers (61,62).…”

Section: Functional Roles Of Lncrnas In Ccamentioning

confidence: 99%

“…Survivin is a protein that inhibits apoptosis and upregulated in cancers (61,62). FENDRR repressed proliferation, migration, and invasion of CCA cell lines HuCCT1 and QBC939 cells via regulation of survivin (60). An in vitro study using CCA cell lines (HuCCT1, Huh-28, KKU-214, and RBE) has demonstrated that CCA cells express elevated levels of lncRNA LINC01061 (63).…”

Section: Functional Roles Of Lncrnas In Ccamentioning

confidence: 99%

“…For example, expression levels of lncRNA-NEF and FENDRR are downregulated in CCA tissues compared to normal tissues (54,60). Overexpression of these lncRNAs inhibited cell migration and invasion of CCA cell lines HuCCT1, QBC939, or TFK-1 cells, indicating the potentials of lncRNA induction as another therapeutic approach for CCA (54,60). As well as lncRNA silencing, lncRNA induction has same limitations: (i) Current studies are limited in the use of in vitro cultured CCA cell lines; and (ii) Technical difficulties to induce specific lncRNAs expression in specific cell types such as CCA cells.…”

Section: Induction Of Lncrna Expressionmentioning

confidence: 99%

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Concise Review: Functional Roles and Therapeutic Potentials of Long Non-coding RNAs in Cholangiopathies

et al. 2020

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Long non-coding RNAs (lncRNAs) are RNAs with lengths exceeding 200 nucleotides that are not translated into proteins. It is well-known that small non-coding RNAs, such as microRNAs (miRNAs), regulate gene expression and play an important role in cholangiopathies. Recent studies have demonstrated that lncRNAs may also play a key role in the pathophysiology of cholangiopathies. Patients with cholangiopathies often develop cholangiocarcinoma (CCA), which is cholangiocyte-derived cancer, in the later stage. Cholangiocytes are a primary target of therapies for cholangiopathies and CCA development. Previous studies have demonstrated that expression levels of lncRNAs are altered in the liver of cholangiopathies or CCA tissues. Some lncRNAs regulate gene expression by inhibiting functions of miRNAs leading to diseased liver conditions or CCA progression, suggesting that lncRNAs could be a novel therapeutic target for those disorders. This review summarizes current understandings of functional roles of lncRNAs in cholangiopathies and seek their potentials for novel therapies.

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Emerging roles of long noncoding RNAs in cholangiocarcinoma: Advances and challenges

Yang

Deng

et al. 2020

Cancer Communications

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Cholangiocarcinoma (CCA), a cancer with a relatively low incidence rate, is usually associated with poor prognosis. Current modalities for the diagnosis and treatment of CCA patients are still far from satisfactory. In recent years, numerous long noncoding RNAs (lncRNAs) have been identified as crucial players in the development of various cancers, including CCA. Abnormally expressed lncRNAs in CCA, regulated by some upstream molecules, significantly influence the biological behavior of tumor cells and are involved in tumor development through various mechanisms, including interactions with functional proteins, participation in competing for endogenous RNA (ceRNA) regulatory networks, activation of cancer‐related signaling pathways and epigenetic modification of gene expression. Furthermore, several lncRNAs are closely associated with the clinicopathological features of CCA patients, and are promising biomarkers for diagnosing and prognostication of CCA. Some of these lncRNAs play an important role in chemotherapy drug resistance. In addition, lncRNAs have also been shown to be involved in the inflammation microenvironment of CCA and malignant outcome of CCA risk factors, such as cholestatic liver diseases. In view of the difficulty of diagnosing CCA, more attention should be paid to detectable lncRNAs in the serum or bile. This review summarizes the recent knowledge on lncRNAs in CCA and provides a new outlook on the molecular mechanisms of CCA development from the perspective of lncRNAs. Moreover, we also discussed the limitations of the current studies and differential expression of lncRNAs in different types of CCA.

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LncRNA FENDRR represses proliferation, migration and invasion through suppression of survivin in cholangiocarcinoma cells

Cited by 26 publications

References 27 publications

FENDRR suppresses cervical cancer proliferation and invasion by targeting miR-15a/b-5p and regulating TUBA1A expression

FENDRR suppresses cervical cancer proliferation and invasion by targeting miR-15a/b-5p and regulating TUBA1A expression

Concise Review: Functional Roles and Therapeutic Potentials of Long Non-coding RNAs in Cholangiopathies

Emerging roles of long noncoding RNAs in cholangiocarcinoma: Advances and challenges

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