LncRNA FBXL19-AS1 promotes proliferation and metastasis of cervical cancer through upregulating COL1A1 as a sponge of miR-193a-5p

Abstract

Background Cervical cancer (CC) is one of the most common and malignant tumors in women. In this study, we aim to explore the role and mechanism of F-box and leucine rich repeat protein 19 antisense RNA 1 (FBXL19-AS1), a novel long-chain non coding RNA (lncRNA) with marked roles in a variety of tumors, in regulating the proliferation and metastasis of CC. Methods The expression of FBXL19-AS1, miR-193a-5p and COL1A1 were detected by RT-PCR and western blot. Gain- and loss-of functional assays of FBXL19-AS1 and miR-193a-5p were performed in CC cell lines in vitro or in vivo. The proliferation, migration, invasion, apoptosis and epithelial-mesenchymal transition (EMT) of CC cells were determined. Results FBXL19-AS1 and COL1A1 were significantly up-regulated in CC tissues, while miR-193a-5p was significantly down-regulated. Overexpression of FBXL19-AS1 significantly promoted the proliferation, migration, invasion, EMT and growth of CC cells and inhibited apoptosis, while knockdown of FBXL19-AS1 had the opposite effects. On the other hand, miR-193a-5p inhibited the proliferation and metastasis of CC cells. Mechanistically, FBXL19-AS1 functioned as a competitive endogenous RNA (ceRNA) and inhibited the expression of miR-193a-5p, which targeted at the 3’-UTR site of COL1A1 and negatively regulated COL1A1 expression. Conclusions FBXL19-AS1 promotes the proliferation and metastasis of CC cells by sponging miR-193a-5p and up-regulating COL1A1.

Circular RNA circACAP2 Suppresses Ferroptosis of Cervical Cancer during Malignant Progression by miR-193a-5p/GPX4

“…Moreover, it has been reported that lncRNA FBXL19-AS1 regulates invasion, migration, and growth of cervical cancer by miR-193a-5p/PIN1 axis [23]. lncRNA FBXL19-AS1 enhances metastasis and proliferation by miR-193a-5p/ COL1A1 axis in cervical cancer [24]. e depletion of SFRS9 represses colorectal cancer progression by enhancing ferroptosis by GPX4 [25].…”

“…e function of circACAP2 in the regulation of other types of programmed cell death such as apoptosis and pyroptosis should be validated in future investigations. Meanwhile, it has been identified that miR-193a-5p plays a critical role in the development of cervical cancer [23,24], and we identified the potential interaction between circACAP2 and miR-193a-5p in the bioinformatic analysis. However, as we all know, circRNAs target multiple downstream miRNAs and other miRNAs sponged by circACAP2 need to be identified in cervical cancer progression.…”

LncRNA FLVCR1-AS1 mediates miR-23a-5p/SLC7A11 axis to promote malignant behavior of cervical cancer cells

“…Culture of cells was in Roswell Park Memorial Institute (RPMI) 1640 (Gibco, NY, USA) medium covering 10% fetal bovine serum (FBS) (Gibco, NY, USA) and 1% penicillin/streptomycin (Gibco, NY, USA). Selection of Hela cells is as the auxiliary research object owing to its extremely elevated difference [ 2 ].…”

“…Presently, the critical clinical treatment strategies for CC cover surgery, chemotherapy, and radiotherapy. Nevertheless, radiotherapy and chemotherapy are available to kill a considerable number of normal cells owing to the limitation of the adaptation stage of surgical cure [ 2 ]. Nevertheless, these traditional therapy methods are unable to effectively cure CC, and infiltration, metastasis, and recurrence remain the crucial reason of death [ 3 ].…”

“…Numerous evidences elaborate lncRNA is the crucial regulator of CC progression. For instance, lncRNA FBXL19-AS1, as the sponge of microRNA (miRNA, miR)-193a-5p, boosts the advancement of CC via elevating COL1A1 [ 2 ]. LncRNA LIPE-AS1 forecasts the declined survival rate of CC and facilitates its progression via modulating the miR-195-5p/MAPK pathway [ 7 ].…”