LncRNA DSCAM-AS1 promotes colorectal cancer progression by acting as a molecular sponge of miR-384 to modulate AKT3 expression

Li, Bo; Sun, Hai; Zhang, Jiayu

doi:10.18632/aging.103243

Cited by 21 publications

(20 citation statements)

References 32 publications

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“…Recent studies indicated that AKT3 is distinctly overexpressed in CRC and that lncRNAs play an oncogenic role in CRC by functioning as a ceRNAs to modulate AKT3 expression 32 , 33 . In this research, a series of experiments proved that AKT3 is a target of miR-576-5p and is reciprocally associated with miR-576-5p.…”

Section: Discussionmentioning

confidence: 99%

Silencing long non-coding RNA CASC9 inhibits colorectal cancer cell proliferation by acting as a competing endogenous RNA of miR-576-5p to regulate AKT3

et al. 2020

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Increasing studies have shown that long non-coding RNAs (lncRNAs) are regarded as important regulators in the occurrence and development of colorectal cancer (CRC). Although lncRNA CASC9 has been studied in CRC, the detailed regulatory mechanism of CASC9 in CRC is still unclear. In this study, we found that CASC9 was significantly upregulated in CRC tissues and cell lines compared to normal controls and that aberrant expression was associated with the tumor-node-metastasis (TNM) stage of CRC. Functionally, CASC9 depletion efficiently inhibited the proliferation of CRC cells and induced cell apoptosis in vitro. Mechanistically, CASC9 was mainly enriched in the cytoplasm of CRC cells and interacted directly with miR-576-5p. Downregulation of miR-576-5p reversed the inhibitory effect of CASC9 siRNA on CRC cell progression. Furthermore, AKT3 has been identified as a downstream target of miR-576-5p. Spearman’s correlation analysis revealed that AKT3 was negatively correlated with miR-576-5p but positively correlated with CASC9. Downregulation of miR-576-5p restored the effect of CASC9 silencing on AKT3 expression. Therefore, silencing CASC9 could downregulate the expression of AKT3 by reducing the competitive binding of CASC9 to miR-576-5p, thus suppressing CRC cell proliferation and promoting cell apoptosis. In summary, we identified CASC9 as an oncogenic lncRNA in CRC and defined the CASC9/miR-576-5p/AKT3 axis, which might be considered a potential therapeutic target for CRC patients, as a novel molecular mechanism implicated in the proliferation and apoptosis of CRC.

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Section: Discussionmentioning

confidence: 99%

Silencing long non-coding RNA CASC9 inhibits colorectal cancer cell proliferation by acting as a competing endogenous RNA of miR-576-5p to regulate AKT3

et al. 2020

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“…Notably, recent studies have revealed that lncRNAs can affect the malignant behavior of cells (15,16). In addition, numerous studies have revealed that DSCAM-AS1 can affect tumorigenesis, but its role in EC remains unclear (7)(8)(9)17,18). Therefore, the aim of the present study was to investigate the biological functions of DSCAM-AS1 in EC.…”

Section: Discussionmentioning

confidence: 99%

“…After surgery, patients were treatment with radiotherapy, chemotherapy, or combination therapies. Estrogen receptor (ER), Lymph-vascular space invasion (LVSI) and histology type were classified by pathologists at our hospital in a blinded manner according to the criteria of previous literature (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13). Written informed consent was obtained from all enrolled patients.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Down syndrome cell adhesion molecule antisense 1 (DSCAM-AS1) was reported to serve as an oncogenic lncRNA in different cancer types (7)(8)(9). High DSCAM-AS1 levels were identified as a predictor for the overall survival of patients with colorectal cancer (7). Moreover, DSCAM-AS1 was determined to stimulate non-small cell lung cancer progression by regulating high mobility group box protein 1 expression via sponging microRNA (miR)-577 (8).…”

Section: Introductionmentioning

confidence: 99%

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lncRNA DSCAM‑AS1 facilitates the progression of endometrial cancer via miR‑136‑5p

Chen

Huang

et al. 2021

Oncol Lett

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Previous studies have indicated that long non-coding RNA (lncRNA) down syndrome cell adhesion molecule antisense 1 (DSCAM-AS1) serves an oncogenic role in numerous cancer types. However, its role in endometrial cancer (EC) remains largely unknown. In the present study, DSCAM-AS1 expression levels in EC tissues and cells and their normal counterparts were analyzed using reverse transcription-quantitative. In vitro and in vivo experiments were conducted to validate the functions of DSCAM-AS1 in EC. It was revealed that DSCAM-AS1 was expressed at a high level in EC tissues and cells after analyzing patient data and data obtained from The Cancer Genome Atlas. Notably, it was also revealed that high DSCAM-AS1 expression was associated with a less favorable overall survival in patients with EC. Knockdown of DSCAM-AS1 was able to suppress EC cell proliferation by upregulating cell apoptosis in vitro. Furthermore, it was revealed that DSCAM-AS1 acted as a microRNA (miR)-136-5p sponge to exert its oncogenic roles in EC. Collectively and to the best of our knowledge, the current results provided first evidence that DSCAM-AS1 stimulated EC progression by regulating miR-136-5p, which may improve the understanding of the roles of ncRNAs in EC, and may help identify novel targets for anticancer treatment.

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Astragalus mongholicus Bunge-Curcuma aromatica Salisb. suppresses growth and metastasis of colorectal cancer cells by inhibiting M2 macrophage polarization via a Sp1/ZFAS1/miR-153-3p/CCR5 regulatory axis

Sun

Tang

et al. 2022

Cell Biol Toxicol

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LncRNA DSCAM-AS1 promotes colorectal cancer progression by acting as a molecular sponge of miR-384 to modulate AKT3 expression

Cited by 21 publications

References 32 publications

Silencing long non-coding RNA CASC9 inhibits colorectal cancer cell proliferation by acting as a competing endogenous RNA of miR-576-5p to regulate AKT3

Silencing long non-coding RNA CASC9 inhibits colorectal cancer cell proliferation by acting as a competing endogenous RNA of miR-576-5p to regulate AKT3

lncRNA DSCAM‑AS1 facilitates the progression of endometrial cancer via miR‑136‑5p

Astragalus mongholicus Bunge-Curcuma aromatica Salisb. suppresses growth and metastasis of colorectal cancer cells by inhibiting M2 macrophage polarization via a Sp1/ZFAS1/miR-153-3p/CCR5 regulatory axis

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