LncRNA ANRIL/miR-7-5p/TCF4 axis contributes to the progression of T cell acute lymphoblastic leukemia

Abstract

Background Antisense non-coding RNA in the INK4 locus (ANRIL) is of great importance in cell biological behaviors, and ANRIL functions in many kinds of cancers including leukemia. However, the mechanism of ANRIL in the progression of T-cell acute lymphoblastic leukemia (T-ALL) has not been clarified clearly. Methods qRT-PCR was performed to detect ANRIL expression in T-ALL samples. T-ALL cell lines (MOLT4, CCRF-CEM and KOPT-K1) were used as the cell models. The function of ANRIL on T-ALL cells was investigated by CCK-8 assays, Transwell assays, and apoptosis experiments in vitro. qRT-PCR, Western blot, luciferase reporter assay and RIP assay were used to confirm the interactions between ANRIL and miR-7-5p, miR-7-5p and its target gene transcription factor 4 (TCF4). Results ANRIL was significantly up-regulated in T-ALL samples. Its knockdown markedly inhibited viability, migration and invasion of T-ALL cells, but its overexpression exerted the opposite effects. TCF4 was proved to be a target gene of miR-7-5p. ANRIL down-regulated miR-7-5p via sponging it and in turn up-regulated TCF4. Conclusions LncRNA ANRIL can modulate malignant phenotypes of T-ALL cells, possibly by regulating miR-7-5p/TCF4 axis, and it serves as a potential therapeutic target for T-ALL.

Downregulation of lncRNA ANRIL Inhibits Osteogenic Differentiation of Periodontal Ligament Cells via Sponging miR-7 through NF-κB Pathway

“…Shu et al have reported that silence of ANRIL exacerbates H9c2 cell injury induced by hypoxia by miR-7-5p/SIRT1 axis [27]. Li et al have revealed that ANRIL mediates the migration and invasion of T-cell acute lymphoblastic leukemia via miR-7-5p/TCF4 axis [28]. In our study, we also veri ed that ANRIL sponge to miR-7 through dualluciferase reporter assay.…”

LINC00511 facilitates Temozolomide resistance of glioblastoma cells via sponging miR‐126‐5p and activating Wnt/β‐catenin signaling

“…Besides this, there are many lncRNAs located both in the cytoplasm and nucleus, and act as sponges for microRNAs, such as lncRNA ANRIL. [44] In our study, we found that LINC00511 regulated Wnt/β-catenin signaling by sponging miR-126-5p. Indeed, LINC00511 has been reported to regulate the proliferation and apoptosis of bladder cancer cells via Wnt/β-catenin signaling.…”

“…Besides this, there are many lncRNAs located both in the cytoplasm and nucleus, and act as sponges for microRNAs, such as lncRNA ANRIL. [ 44 ]…”

Oxaliplatin Induces Cell Cycle Arrest and Apoptosis in T-leukemia Cells through Mitochondrial and PI3K/Akt Signaling Pathways

“…T-cell acute lymphoblastic leukemias (T-ALL) is a common hematological malignant tumor in children, accounting for 80% of childhood leukemia cases, and it is also the second most common acute leukemia in adults. [4,5] In the past 20 years, the complete remission rate of initial treatment has remained low, while the fatality rate was high. [6] The 5-year survival rate is about 30% for adults and about 90% for children.…”