LncRNA AFAP1-AS1/miR-27b-3p/VEGF-C axis modulates stemness characteristics in cervical cancer cells

Xia, Meng; Duan, Lijun; Lu, Bingwei; Pang, Yuzhou; Pang, Zhigang

doi:10.1097/cm9.0000000000001665

Cited by 11 publications

(4 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most of these miRNAs showed an inhibitory effect on CC. Hsa-miR-27b-3p upregulation could suppress the CC cell stemness by suppressing VEGF-C [29]. Has-miR-495-3p was reported to suppress the occurrence and development of CC through downregulating the CDK1 expression [30].…”

Section: Discussionmentioning

confidence: 99%

Prognostic value and mechanism of EPHB2 overexpression in cervical cancer

Zhao

Cong

et al. 2022

Preprint

View full text Add to dashboard Cite

Background Cervical cancer (CC) still represents an important challenge for female malignant tumors. In recent years, increasing evidence has indicated that EPHB2 plays a significant part in the origin and progression of many types of cancer in humans. Nevertheless, the mechanism of EPHB2 in CC remains inexplicable. The purpose of this study is to determine the prognostic value and related regulatory mechanism of EPHB2 in CC. Methods The basic clinical information and transcriptome data related to CC patients were downloaded from TCGA database and conducted standardization processing. TIMER2.0 was used for analyzing the EPHB2 expression differences of between 38 cancer types and corresponding normal tissues. The expression and prognostic value (including OS and RFS) of EPHB2 in different cancer types were determined by GEPIA2. And the starbase database was used for exploring the upstream miRNAs and lncRNAs of EPHB2. Then, The correlation analysis of EPHB2, miRNA and lncRNA and the exploration of prognostic value and expression of miRNAs in CESC was conducted using the R language packages. We also analyzed the prognostic value and expression difference of 187 lncRNAs based on the UALCAN database. Finally, we performed the correlation analysis between EPHB2 and multiple immune cells using the R language package. Results In our study, the AC073957.3/hsa-miR-150-5p axis was found to be the most potential upstream ncRNA-related regulator of EPHB2 in CC. And the results of immune correlation infiltration analysis suggest that as EPHB2 copy numbers fluctuate in CESC there is an obvious difference in immune cell infiltration levels, and EPHB2 was markedly positively correlated with Th2 cell, NK cell, Eosinophils, Tgd, Mast cell, NK CD56bright cells and Tem while negatively correlated with Tcm and B cell in CC. Conclusion Our study shows that EPHB2 is a potential prognostic marker, and its overexpression is related to poor prognosis of CC, which is expected to be a new therapeutic target for CC.

show abstract

Section: Discussionmentioning

confidence: 99%

Prognostic value and mechanism of EPHB2 overexpression in cervical cancer

Zhao

Cong

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…A number of studies have shown that in cervical cancer, lncRNAs competitively decoy miRNAs via miRNA response elements (MREs), and this process reduces the binding of miRNAs to their target mRNAs, which indirectly upregulates the level of downstream mRNAs [28][29][30].…”

Section: Discussionmentioning

confidence: 99%

LncRNA FAM13A-AS1 Regulates Proliferation and Apoptosis of Cervical Cancer Cells by Targeting miRNA-205-3p/DDI2 Axis

Qiu

et al. 2022

Journal of Oncology

View full text Add to dashboard Cite

The aim of this study was to explore the function of long noncoding RNA (lncRNA) FAM13A-AS1 and its associated mechanism in cervical cancer. A total of 30 cervical cancer tissues and adjacent tissues were collected. Cervical cancer cell lines, including SiHa and HeLa, were transfected with constructs expressing LV-FAM13A-AS1, silencing RNA LV-siFAM13A-AS1, miRNA mimics, and miRNA inhibitors. RT-qPCR was used to detect the expression of FAM13A-AS1 in cervical cancer tissues, including SiHa, HeLa, and HUCEC cells. MTT, flow cytometry, and transwell assays were performed to explore the influence of FAM13A-AS1 on cervical cancer cell proliferation, apoptosis, invasion, and migration. A bioinformatics analysis and a dual-luciferase assay were carried to confirm the target relationship between FAM13A-AS1 or DDI2 and miRNA-205-3p. Finally, in vivo tumorigenesis experiments were performed in nude mice to explore the effect of FAM13A-AS1 expression on cervical cancer. Low FAM13A-AS1 expression and high miRNA-205-3p expression were observed in cervical cancer tissues and cell lines (SiHa and HeLa). Upregulating the expression of FAM13A-AS1 inhibited proliferation, migration, and invasion of SiHa and HeLa cells, while the apoptosis of SiHa and HeLa cells was increased. More importantly, LV-FAM13A-AS1 could improve tumor development in vivo. In addition, FAM13A-AS1 negatively regulated the expression of miRNA-205-3p, while miRNA-205-3p reduced DDI2 expression, and miRNA-205-3p mimic reversed the effects of FAM13A-AS1 overexpression in vitro. In conclusion, FAM13A-AS1 inhibits the progression of cervical cancer by targeting the miRNA-205-3p/DDI2 axis, suggesting that FAM13A-AS1 might be a potential target for cancer cell treatment.

show abstract

“…A similar mechanism involving the activation of VEGF-C expression via microRNA sponging is employed by the lncRNA AFAP1-AS1, which stands for actin filament-associated protein 1 antisense RNA 1. AFAP1-AS1 is an oncogenic lncRNA [ 115 ] that, according to a recent study by Xia et al [ 116 ], acts as a sponge for miR-27b-3p in cervical cancer cells, sequestering it away from its target VEGF-C . The study suggested that the derepression of VEGF-C by AFAP1-AS1 could be responsible for promoting lymphatic metastasis and enhancing cervical cancer stemness.…”

Section: Lncrnas As Regulators Of the Lymphangiogenic Growth Factor V...mentioning

confidence: 99%

The emerging roles of long noncoding RNAs in lymphatic vascular development and disease

Ivanov

Samuilova

Zamyatnin

2023

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Recent advances in RNA sequencing technologies helped uncover what was once uncharted territory in the human genome—the complex and versatile world of long noncoding RNAs (lncRNAs). Previously thought of as merely transcriptional “noise”, lncRNAs have now emerged as essential regulators of gene expression networks controlling development, homeostasis and disease progression. The regulatory functions of lncRNAs are broad and diverse, and the underlying molecular mechanisms are highly variable, acting at the transcriptional, post-transcriptional, translational, and post-translational levels. In recent years, evidence has accumulated to support the important role of lncRNAs in the development and functioning of the lymphatic vasculature and associated pathological processes such as tumor-induced lymphangiogenesis and cancer metastasis. In this review, we summarize the current knowledge on the role of lncRNAs in regulating the key genes and pathways involved in lymphatic vascular development and disease. Furthermore, we discuss the potential of lncRNAs as novel therapeutic targets and outline possible strategies for the development of lncRNA-based therapeutics to treat diseases of the lymphatic system.

show abstract

LncRNA AFAP1-AS1/miR-27b-3p/VEGF-C axis modulates stemness characteristics in cervical cancer cells

Cited by 11 publications

References 45 publications

Prognostic value and mechanism of EPHB2 overexpression in cervical cancer

Prognostic value and mechanism of EPHB2 overexpression in cervical cancer

LncRNA FAM13A-AS1 Regulates Proliferation and Apoptosis of Cervical Cancer Cells by Targeting miRNA-205-3p/DDI2 Axis

The emerging roles of long noncoding RNAs in lymphatic vascular development and disease

Contact Info

Product

Resources

About