Lnc-PCDH9-13:1 Is a Hypersensitive and Specific Biomarker for Early Hepatocellular Carcinoma

Xie, Zhenghui; Zhou, Fangyuan; Yang, Yidong; Li, Leijia; Lei, Yiming; Lin, Xiao; Li, Weiwei; Pan, Xuemei; Chen, Jianning; Wang, Genshu; Liu, Huiling; Jiang, Jie; Wu, Bin

doi:10.1016/j.ebiom.2018.06.026

Cited by 50 publications

(46 citation statements)

References 29 publications

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“…MiR-494-3p as a carcinogenic factor was upregulated in lung cancer and promoted the progression of lung cancer ( 21 ). Based on the competing endogenous RNA (ceRNA) hypothesis and the fact that LINC00355 acts as a carcinogenic factor in several cancers ( 15 – 18 ), miR-466 was thus selected for the following analysis.…”

Section: Methodsmentioning

confidence: 99%

“…It has no protein-coding capacity, and can interact with chromatin and histone modifiers, thereby regulate gene expression ( 14 ). Several reports suggest that the abnormal expression of LINC00355 is linked to the overall survival of cancers such as prostate cancer, colorectal cancer, and colon adenocarcinoma ( 15 – 18 ). LINC00355 is regarded as a good candidate for understanding the mechanisms of sepsis ( 19 ).…”

Section: Introductionmentioning

confidence: 99%

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LINC00355 promoted the progression of lung squamous cell carcinoma through regulating the miR-466/LYAR axis

Sun

Wang

Ding

et al. 2020

Braz J Med Biol Res

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LINC00355 has been reported aberrantly over-expressed and associated with poor prognosis in various types of cancer. However, reports regarding the effect of LINC00355 on lung squamous cell carcinoma (SCC) are rare. This study aimed to explore the function of LINC00355 in the development and progression of lung SCC and reveal the underlying mechanism. The expression and subcellular location of LINC00355 were determined by qRT-PCR and RNA-FISH, respectively. The lung SCC cell growth was analyzed by CCK-8 assay, transwell invasion, wound healing, colony formation, and flow cytometry assays. Reactive oxygen species level was evaluated by DCFH-DA probes. Bioinformatics online websites, luciferase reporter assay, RNA binding protein immunoprecipitation (RIP), and RNA pull-down assays were utilized to investigate the interaction among LINC00355, miR-466, and Ly-1 antibody reactive clone (LYAR). The results showed that LINC00355 was upregulated in lung SCC and was positively associated with poor overall survival in lung SCC patients. LINC00355 was mainly located in the cytoplasm of SCC cells. Additionally, LINC0035 functioned as a competing endogenous RNA (ceRNA) to target miR-466, and LYAR was identified as a direct target of miR-466. LINC00355 expression negatively correlated with miR-466 level, and positively correlated with LYAR level. Mechanistically, knockdown of LINC00355 inhibited cell proliferation, migration and invasion, promoted cell apoptosis in vitro , and suppressed tumor growth in vivo through targeting miR-466, and thus down-regulated LYAR expression. These findings provide a new sight for understanding the molecular mechanism of lung SCC and indicate that LINC00355 may serve as a potential biomarker for the diagnosis and treatment of lung SCC.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

LINC00355 promoted the progression of lung squamous cell carcinoma through regulating the miR-466/LYAR axis

Sun

Wang

Ding

et al. 2020

Braz J Med Biol Res

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“…In addition to the investigation and recognition of lncRNAs, there is increasing evidence that they have significant roles as tumor oncogenes or/and suppressors (8)(9)(10). Furthermore, several lncRNAs are important in the progression and development of liver cancer (11,12).…”

Section: Introductionmentioning

confidence: 99%

Long non‑coding RNA GACAT3 promotes liver cancer progression by regulating the proliferation, apoptosis and migration of tumor cells

et al. 2020

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Long non-coding RNA gastric cancer associated transcript 3 (GACAT3), is a newly identified non-coding RNA, which has been found to be involved in the tumorigenesis of gastric cancer. However, the biological function of GACAT3 in liver cancer remains unclear. The present study aimed to determine the expression level and function of GACAT3 in liver cancer. The authors cultured liver cancer cells in vitro and GACAT3 was silenced in the cells. Cell proliferation, apoptosis and migration were determined by MTT assay, flow cytometric analysis and transwell assay, respectively. It was demonstrated that GACAT3 was upregulated in liver cancer tissues. The inhibition of GACAT3 decreased the ability of hepatoma cells to proliferate and migrate, and increased apoptosis of the cells. These findings provide the first evidence, to the best of our knowledge, of the exact role of GACAT3 in liver cancer, suggesting GACAT3 as a potential target for liver cancer therapy in the future.

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“…Previous studies demonstrated that PCDH9 was down-regulated in cancers, such as glioblastoma, hepatocellular carcinoma and gastric cancer. [35][36][37][38] Overexpression of PCDH9 inhibited the proliferation, migration and invasion of cancer cells. However, the function of PCDH9 in ovarian cancer remains largely unknown.…”

Section: Introductionmentioning

confidence: 99%

<p>MiR-200a-3p promoted the malignant behaviors of ovarian cancer cells through regulating PCDH9</p>

Shi¹,

Yang²,

Zhang³

et al. 2019

OTT

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Background: Increasing evidence has revealed that the aberrant expression of microRNAs (miRNAs) plays vital roles in the development and progression of ovarian cancer. MiR-200a-3p was found to act as an oncogene in a variety of cancers, however, the expression and function of miR-200a-3p in ovarian cancer has not been characterized. Materials and methods: The expression of miR-200a-3p in ovarian cancer tissues and cell lines was detected by the RT-qPCR. The influence of miR-200a-3p on the growth of ovarian cancer cells was determined with the Cell Counting Kit-8 assay, colony formation and cell invasion assay. The binding of miR-200a-3p with the 3ʹ-untranslated region (UTR) of PDCH9 was detected by luciferase reporter assay. The expression of PCDH9 was investigated by RT-qPCR and Western blot analysis. Results: miR-200a-3p was up-regulated in ovarian cancer tissues and cell lines. Highly expressed miR-200a-3p was significantly associated with the tumor size, tumor metastasis and TNM stage. Overexpression of miR-200a-3p markedly promoted the proliferation, colony formation and invasion of ovarian cancer cells. Functional study uncovered that miR-200a-3p bound the 3ʹ-untranslated region (UTR) of PCDH9 and decreased the expression of PCDH9 in ovarian cancer cells. The expression of miR-200a-3p in ovarian cancer tissues was significantly negatively correlated with that of PCDH9. Restored PCDH9 inhibited the promoting effect of miR-200a-3p on the proliferation of ovarian cancer cells. Conclusion: Our results suggested the potential oncogenic function of miR-200a-3p via modulating PCDH9 in ovarian cancer.

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Lnc-PCDH9-13:1 Is a Hypersensitive and Specific Biomarker for Early Hepatocellular Carcinoma

Cited by 50 publications

References 29 publications

LINC00355 promoted the progression of lung squamous cell carcinoma through regulating the miR-466/LYAR axis

LINC00355 promoted the progression of lung squamous cell carcinoma through regulating the miR-466/LYAR axis

Long non‑coding RNA GACAT3 promotes liver cancer progression by regulating the proliferation, apoptosis and migration of tumor cells

<p>MiR-200a-3p promoted the malignant behaviors of ovarian cancer cells through regulating PCDH9</p>

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