Lnc-mg is a long non-coding RNA that promotes myogenesis

Zhu, Mu; Liu, Jiafan; Xiao, Jia; Yang, Li; Cai, Meili; Shen, Huan; Chen, Xiaojia; Ma, Yi; Hu, Shengdi; Wang, Zuolin; Hong, An; Li, Yingxian; Sun, Ying; Wang, Xiaogang

doi:10.1038/ncomms14718

Cited by 171 publications

(175 citation statements)

References 57 publications

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“…Long non‐coding RNAs (lncRNAs) have been demonstrated to regulate muscle differentiation, including H19, linc‐MD1, Yam‐1, Malat1, and lnc‐mg . In current study, the newly identified lncRNA MAR1 was highly expressed in skeletal muscle and associated with the changes of muscle growth during either age‐related or mechanical loading‐related changes in mice, implying its potential function in regulating muscle differentiation and regeneration.…”

Section: Discussionmentioning

confidence: 74%

A newly identified lncRNA MAR1 acts as a miR‐487b sponge to promote skeletal muscle differentiation and regeneration

Zhang

Guan

et al. 2018

J cachexia sarcopenia muscle

141

126

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Background Skeletal muscle atrophy induced by either aging (sarcopenia) or mechanical unloading is associated with serious health consequences. Long non-coding RNAs (lncRNAs) are implicated as important regulators in numerous physiological and pathological processes. Methods Microarray analysis was performed to identify the differentially expressed lncRNAs in skeletal muscle between adult and aged mice. The most decreased lncRNA in aged skeletal muscle was identified. The C2C12 mouse myoblast cells were used to assess the biological function of the lncRNA in vitro. The target microRNA of lncRNA and the target protein of microRNA were predicted by bioinformatics analysis and validated in vitro. Furthermore, the biology function of the lncRNA in vivo was investigated by local overexpression or knockdown the lncRNA in skeletal muscle. The therapeutic effect of the lncRNA overexpression in age-related or mechanical unloading-induced muscle atrophy was also evaluated. Results We identified a novel lncRNA (muscle anabolic regulator 1, MAR1) which was highly expressed in mice skeletal muscle and positively correlated with muscle differentiation and growth in vitro and in vivo. We predicted and validated that microRNA-487b (miR-487b) was a direct target of MAR1. We also predicted and validated that Wnt5a, an important regulator during myogenesis, was a target of miR-487b in C2C12 cells. Our findings further demonstrated that enforced MAR1 expression in myoblasts led to derepression of Wnt5a. Moreover, MAR1 promoted skeletal muscle mass/strength and Wnt5a protein level in mice. Enforced MAR1 expression in mice attenuated muscle atrophy induced by either aging or unloading. Conclusions The newly identified lncRNA MAR1 acts as a miR-487b sponge to regulate Wnt5a protein, resulting in promoting muscle differentiation and regeneration. MAR1 could be a novel therapeutic target for treating muscle atrophy induced by either aging or mechanical unloading.

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Section: Discussionmentioning

confidence: 74%

A newly identified lncRNA MAR1 acts as a miR‐487b sponge to promote skeletal muscle differentiation and regeneration

Zhang

Guan

et al. 2018

J cachexia sarcopenia muscle

141

126

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“…Lnc-mg is necessary for myogenic differentiation. Through interaction with miR-125b, lnc-mg promotes myoblast differentiation (20). Malat1 is important for the transition between the proliferation and differentiation stages of myogenesis (21).…”

Section: Lncrnas In Myogenesis (Table 1)mentioning

confidence: 99%

Long noncoding RNAs and their roles in skeletal muscle fate determination

Hagan

Zhou

Ashraf

et al. 2017

Non-coding RNA Investig

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Myogenic fate determination is important in skeletal muscle development, growth and repair. A variety of factors regulate myogenic cell determination via transcriptional and non-transcriptional mechanisms. Amongst these factors, long noncoding RNAs (lncRNAs) have gained considerable attention for their important roles in regulating myogenic differentiation and function. Many classes of lncRNAs have been discovered; various lncRNAs have been implicated in the regulation of myogenic cell fate determination and are the subject of this brief review.

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“…lncRNAs have been reported to be involved in a variety of biological processes, including tumorigenesis through regulating oncogenes or tumor suppressors at several levels (3)(4)(5)(6)(7)(8)(9)(10)(11)(12). For example, Xia et al (13) demonstrated that long noncoding RNA papillary thyroid carcinoma susceptibility candidate 3 (PTCSC3) inhibited proliferation and invasion of glioma cells by suppressing the Wnt/β-catenin signaling pathway.…”

Section: Introductionmentioning

confidence: 99%

lncRNA FAM83H‑AS1 is associated with the prognosis of colorectal carcinoma and promotes cell proliferation by targeting the Notch signaling pathway

et al. 2017

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Abstract. The long non-coding RNA, FAM83H antisense RNA 1 (head to head) (FAM83H-AS1), has exhibited a functional role as an oncogene in a number of different types of cancer. The aim of the present study was to reveal the dysregulation of FAM83H-AS1 in colorectal carcinoma (CRC) samples and elucidate its underlying associations with the Notch signaling pathway. The expression profiles of FAM83H-AS1 and two Notch signaling-associated molecules, Notch1 and Hes family basic-helix-loop-helix transcription factor 1 (Hes1), were measured by reverse transcription-polymerase chain reaction and western blot analysis. The Pearson χ 2 test was employed to evaluate the associations between FAM83H-AS1 expression and clinical features. A statistically significant positive association between the expression levels of FAM83H-AS1 and those of Notch1 or Hes1 in CRC tissues was analyzed by Spearman's correlation analysis. The Kaplan-Meier method was used to compare the overall survival curves between the highly-expressed and low-expressed FAM83H-AS1 groups via a log-rank test. Specific small hairpin RNA was transfected to silence endogenous FAM83H-AS1. MTT and colony formation assays were performed to measure the growth-inhibition effect of silenced FAM83H-AS1. The levels of FAM83H-AS1, Notch1 and Hes1 were significantly increased in CRC samples and cell lines. Cell proliferation was markedly inhibited when FAM83H-AS1 was knocked down and this effect mediated by FAM83H-AS1 could be reversed by Notch1 regulators. Thus, downregulated FAM83H-AS1 exhibited an anti-proliferative role in CRC by repressing the Notch signaling pathway.

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Lnc-mg is a long non-coding RNA that promotes myogenesis

Cited by 171 publications

References 57 publications

A newly identified lncRNA MAR1 acts as a miR‐487b sponge to promote skeletal muscle differentiation and regeneration

A newly identified lncRNA MAR1 acts as a miR‐487b sponge to promote skeletal muscle differentiation and regeneration

Long noncoding RNAs and their roles in skeletal muscle fate determination

lncRNA FAM83H‑AS1 is associated with the prognosis of colorectal carcinoma and promotes cell proliferation by targeting the Notch signaling pathway

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