LNA-enhanced DNA FIT-probes for multicolour RNA imaging

Hövelmann, Felix; Gáspár, Imre; Chamiolo, Jasmine; Kasper, Marc‐André; Steffen, J.; Ephrussi, Anne; Seitz, Oliver

doi:10.1039/c5sc03053f

Cited by 66 publications

(98 citation statements)

References 55 publications

(52 reference statements)

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“…Notably, this strategy results in no false‐positive signal as a result of probe degradation, overcoming a key limitation associated with MBs . Furthermore, FIT probes offer fluorescence enhancement, reported to be as high as 195‐fold upon addition of complementary target . Much of the work in live cell imaging using FIT probes has relied upon the use of peptide nucleic acids (PNA) due to their biostability and both rapid and strong hybridization to complementary ONTs .…”

Section: Hybridization‐based Probesmentioning

confidence: 99%

“…Therefore, recent work in FIT probes has focused on alternate strategies, for example, by modifying the sugar moieties in ONTs with phosphate backbones . 2′‐O‐Me RNA and LNAs have been used in FIT probes due to their resistance to nuclease degradation and both rapid and high affinity binding to complementary nucleic acids . FIT probes made of DNA have also been used for live cell imaging …”

Section: Hybridization‐based Probesmentioning

confidence: 99%

“…Recently, the Seitz group reported the development of four new dyes for use in FIT probes . Of these, three showed fluorescence enhancement upon addition of complementary target.…”

Section: Hybridization‐based Probesmentioning

confidence: 99%

See 2 more Smart Citations

Nucleic‐Acid Structures as Intracellular Probes for Live Cells

2019

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The chemical composition of cells at the molecular level determines their growth, differentiation, structure, and function. Probing this composition is powerful because it provides invaluable insight into chemical processes inside cells and in certain cases allows disease diagnosis based on molecular profiles. However, many techniques analyze fixed cells or lysates of bulk populations, in which information about dynamics and cellular heterogeneity is lost. Recently, nucleic‐acid‐based probes have emerged as a promising platform for the detection of a wide variety of intracellular analytes in live cells with single‐cell resolution. Recent advances in this field are described and common strategies for probe design, types of targets that can be identified, current limitations, and future directions are discussed.

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Section: Hybridization‐based Probesmentioning

confidence: 99%

Section: Hybridization‐based Probesmentioning

confidence: 99%

See 1 more Smart Citation

Nucleic‐Acid Structures as Intracellular Probes for Live Cells

2019

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“…In the TO/JO-FIT probes, the TO serves as a light collector and transfers the energy to the JO emitter, leading to the successful imaging of oskar mRNA within a complex tissue. Similarly, even brighter LNA-QB probes containing QB (quinoline blue) as the intercalator have a 152-fold fluorescence increase 146 . Further combination with LNA-TO probes allows the detection of oskar mRNA and total mRNA in oocytes of Drosophila melanogaster by means of the wash-free FISH procedure 146 .…”

Section: Probes Imaging Endogenous Rnasmentioning

confidence: 99%

“…(a) Chemical structure of a typical FIT probe 146 and its imaging principle; (b) chemical structure of a typical ECHO probe 150 and its imaging principle.…”

Section: Figmentioning

confidence: 99%

Recent advances in high-performance fluorescent and bioluminescent RNA imaging probes

et al. 2017

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RNA plays an important role in life processes. Imaging of messenger RNAs (mRNAs) and micro-RNAs (miRNAs) not only allows us to learn the formation and transcription of mRNAs and the biogenesis of miRNAs involved in various life processes, but also helps in detecting cancer. High-performance RNA imaging probes greatly expand our view of life processes and enhance the cancer detection accuracy. In this review, we summarize the state-of-the-art high-performance RNA imaging probes, including exogenous probes that can image RNA sequences with special modification and endogeneous probes that can directly image endogenous RNAs without special treatment. For each probe, we review its structure and imaging principle in detail. Finally, we summarize the application of mRNA and miRNA imaging probes in studying life processes as well as in detecting cancer. By correlating the structures and principles of various probes with their practical uses, we compare different RNA imaging probes and offer guidance for better utilization of the current imaging probes and the future design of higher-performance RNA imaging probes.

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Cyanine Conjugate‐Based Biomedical Imaging Probes

Zhou

Yue

et al. 2020

Adv Healthcare Materials

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Cyanine is a class of fluorescent dye with meritorious fluorescence properties and has motivated numerous researchers to explore its imaging capabilities by miscellaneous structural modification and functionalization strategies. The covalent conjugation with other functional molecules represents a distinctive design strategy and has shown immense potential in both basic and clinical research. This review article summarizes recent achievements in cyanine conjugate-based probes for biomedical imaging. Particular attention is paid to the conjugation with targeting warheads and other contrast agents for targeted fluorescence imaging and multimodal imaging, respectively. Additionally, their clinical potential in cancer diagnostics is highlighted and some concurrent impediments for clinical translation are discussed.

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LNA-enhanced DNA FIT-probes for multicolour RNA imaging

Cited by 66 publications

References 55 publications

Nucleic‐Acid Structures as Intracellular Probes for Live Cells

Nucleic‐Acid Structures as Intracellular Probes for Live Cells

Recent advances in high-performance fluorescent and bioluminescent RNA imaging probes

Cyanine Conjugate‐Based Biomedical Imaging Probes

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