“…To avoid possible limitations of LNA probes (e.g., in case of degenerate sequences of pathogens), and to increase targeted enrichment, overlapping primers/LNA probes containing degenerate sequences can be designed and applied, as previously successfully demonstrated [37] . In addition, an increased sensitivity of LNA binding to the target can be obtained by adding LNA blockers [38] or by using LNA probes in combination with ultra-degenerate primers with 3′ termini overlapping the probe-binding site (Pan-Degenerate Amplification and Adaptation (PANDAA)) [39] .…”