lmipenem Resistance in Pseudomonas aeruginosa is Due to Diminished Expression of Outer Membrane Proteins

Büscher, K.-H.; Cullmann, W.; Dick, Wolfgang R.; Wendt, Sebastian; Opferkuch, W.

doi:10.1093/infdis/156.4.681

Cited by 37 publications

(21 citation statements)

References 11 publications

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“…From this study, it is clear that the uropathogens are becoming resistant to the most commonly prescribed antibiotics for uncomplicated UTIs treatment. According to Haque et al, extended sectrum β-lactamase (ESBL) of gram negative uropathogens help them to gain resistance against 3 rd generation cephalosporins [18] whereas resistance to carbapenem antibiotic group is often due to loss of outer membrane proteins and upregulation of active efflux pumps or production of metallo β (MBL) [36]. Major factors known to influence the evolution and transfer of multi drug resistance among microorganisms are incomplete doses, ease of access, over prescription, prescription of higher generation antimicrobials, prescribing antibiotics without laboratory results and indiscriminate use of antimicrobials in agriculture and livestock sectors [37].…”

Section: Fig 6 Antibiogram Profiling Of Staphylococcus Aureus (N=2)mentioning

confidence: 99%

Prevalence and Antibiogram of Bacterial Uropathogens of Urinary Tract Infections from a Tertiary Care Hospital of Bangladesh

Sanjee

Karim²,

Akter³

et al. 2017

J. Sci. Res.

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Urinary tract infections (UTIs) are one of the most frequently occurring infections majority of which are caused by multi-drug resistant (MDR) uropathogens. Hence, the present study was designed to find out the prevalence of bacterial pathogens causing UTIs and to determine their antibiotic resistance patterns against different classes of antibiotics. Cleancatch midstream urine samples were collected from 200 UTI patients of different sex and age groups. The uropathogens were isolated using Hi-Chrome UTI agar, Blood agar, MacConkey agar and then subjected to antibiotic susceptibility analysis against nine antibiotics of different classes using Kirby-Bauer's disc diffusion method. From 55.08% positive samples, it was found that females were more prone to UTIs than males and in both cases; the prevalence rate was higher in the age group 21-40 years (33%). Among the uropathogens, E.coli was the predominant etiological agent (57.38%) followed by Enterococcus sp. (36.06%), Pseudomonas aeruginosa (3.28%) and Staphylococcus aureus (3.28%). The pathogens showed remarkable amount of sensitivity against Gentamicin and Ciprofloxacin. The present experiment can be helpful for the clinicians in finding proper drugs in the developing countries like Bangladesh where multi-drug resistance problem has just complicated the treatment of UTIs.

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Section: Fig 6 Antibiogram Profiling Of Staphylococcus Aureus (N=2)mentioning

confidence: 99%

Prevalence and Antibiogram of Bacterial Uropathogens of Urinary Tract Infections from a Tertiary Care Hospital of Bangladesh

Sanjee

Karim²,

Akter³

et al. 2017

J. Sci. Res.

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“…The role of porins in the permeability of gram-negative aerobic bacteria and in antimicrobial resistance has been extensively studied (23,53,74,91,97,110) and recently reviewed (86,87 (70) showed roles for both ,B-lactamase and the permeability barrier in the activity of cephalosporins against members of the B. fragilis group but found that the species-specific exception was B. distasonis, which showed only a permeability barrier to all antibiotics tested. B. distasonis, though one of the most resistant of the group, has one of the lowest proportions of P-lactamase-positive strains.…”

Section: Issues That Require Further Investigation Endpoint Definitionmentioning

confidence: 99%

Susceptibility testing of anaerobic bacteria: myth, magic, or method?

Wexler

1991

Clin Microbiol Rev

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The demand for susceptibility testing of anaerobes has increased, yet consensus as to procedure and interpretation in this area has not been achieved. While routine testing of anaerobic isolates is not needed, certain isolates in specific clinical settings should be tested. Also, laboratories may monitor their local antibiograms by doing periodic surveillance batch testing. The National Committee for Clinical Laboratory Standards has published a protocol of methods approved for susceptibility testing of anaerobic bacteria. Both agar and broth microdilution are included; however, the broth disk elution method is no longer approved by the National Committee for Clinical Laboratory Standards because of method-related interpretive errors. A number of newer methods are undergoing evaluation and seem promising. Clinicians and microbiologists reviewing susceptibility reports should be aware of sources of variability in the test results. Variables in susceptibility testing of anaerobes include the media and methods used, organisms chosen for testing, breakpoints chosen for interpretation, antibiotic, and determination of endpoint. Clustering of MICs around the breakpoint may lead to significant variability in test results. Adherence of testing laboratories to approved methods and careful descriptions of the method and the breakpoints used for interpretation would facilitate interlaboratory comparisons and allow problems of emerging resistance to be noted. A variety of resistance mechanisms occurs in anaerobic bacteria, including the production of beta-lactamase and other drug-inactivating enzymes, alteration of target proteins, and inability of the drug to penetrate the bacterial wall. Antimicrobial resistance patterns in the United States and abroad are described.

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“…However, during clinical therapy of P. aeruginosa, imipenem-resistant isolates arise at a significant rate (22). The major cause of resistance is loss of a specific outer membrane protein, OprD (5)(6)(7)15 such as meropenem and panipenem (12,31,33). Nevertheless, it was suggested that OprD had significant nonspecific permeability to monosaccharides (and disaccharides) and was generally involved as a porin for antibiotic (13-lactam) uptake (24,33,38,39).…”

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Genetic definition of the substrate selectivity of outer membrane porin protein OprD of Pseudomonas aeruginosa

Huang

Hancock

1993

J Bacteriol

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Earlier studies proved that Pseudomonas aeruginosa OprD is a specific porin for basic amino acids and imipenem. It was also considered to function as a nonspecific porin that allowed the size-dependent uptake of monosaccharides and facilitation of the uptake of quinolone and other antibiotics. In the present study, we utilized P. aeruginosa strains with genetically defined levels of OprD to characterize the in vivo substrate selectivity of this porin. An oprD::fQ interposon mutant was constructed by gene replacement utilizing an in vitro mutagenized cloned oprD gene. In addition, OprD was overexpressed from the lac promoter by cloning the oprD gene into the broad-host-range plasmid pUCP19. To test the substrate selectivity, strains were grown in minimal medium with limiting concentrations of the carbon sources glucose, gluconate, or pyruvate. In minimal medium with 0.5 mM gluconate, the growth rates of the parent strain H103 and its oprD::fQ mutant H729 were only 60 and 20%, respectively, of that of the OprD-overexpressing strain H103(pXH2). In contrast, no significant differences were observed in the growth rates of these three strains on glucose or pyruvate, indicating that OprD selectively facilitated the transport of gluconate. To determine the role of OprD in antibiotic uptake, nine strains representing different levels of OprD and OprF were used to determine the MICs of different antibiotics. The results clearly demonstrated that OprD could be utilized by imipenem and meropenem but that, even when substantially overexpressed, it could not be significantly utilized by other P-lactams, quinolones, or aminoglycosides. In addition, competition experiments confirmed that imipenem had common binding sites with basic amino acids in the OprD channel, but not with gluconate or glucose.

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lmipenem Resistance in Pseudomonas aeruginosa is Due to Diminished Expression of Outer Membrane Proteins

Cited by 37 publications

References 11 publications

Prevalence and Antibiogram of Bacterial Uropathogens of Urinary Tract Infections from a Tertiary Care Hospital of Bangladesh

Prevalence and Antibiogram of Bacterial Uropathogens of Urinary Tract Infections from a Tertiary Care Hospital of Bangladesh

Susceptibility testing of anaerobic bacteria: myth, magic, or method?

Genetic definition of the substrate selectivity of outer membrane porin protein OprD of Pseudomonas aeruginosa

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