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Maralappanavar, M. S.; Kuruvinashetti, M. S.; Harti, Chandrashekhar C.

doi:10.1023/a:1004010315991

Cited by 15 publications

(1 citation statement)

References 7 publications

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“…[6] reported that the highest percentage callus formation was obtained from germinating seeds using 4 mg/l 2,4-D and the callus induction frequency was said to reduce when the concentration of 2,4-D was increased to 6 mg/l. [9] reported highest callus induction frequency from immature inflorescence using 2.0 mg/l 2,4-D + 0.2KN.…”

Section: Callus Inductionmentioning

confidence: 95%

Optimum Protocol for Shoot Formation in karandafi Red Sorghum (<i>Sorghum bicolor</i> (L.) Moench) through Somatic Embryogenesis Using Mature Embryo

.¹,

Usman²,

Katung³

et al. 2014

AJPS

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In this study, an optimum protocol for shoot formation through somatic embryogenesis using mature embryo explants was developed. Calli were initiated on Murashige and Skoog (MS) media supplemented with varying concentration of 2,4-Dichlorophenoxyl acetic acid (2,4-D) ranging from 1.5 mg/l-4.0 mg/l alone or in combination with 0.5 mg/l Kinetin (KN). Significance difference (p < 0.05) was observed among the different concentrations of hormone used for callus induction. The highest percentage callus formation was obtained from the media fortified with 4 mg/l 2, 4-D for mature embryo obtained from imbibed seed while for the preconditioned mature embryo, the media supplemented with 2 m/l 2,4-D + 0.5 mg/l kinetin (KN) recorded more percentage callus formation compared to what was obtained from the media supplemented with 2.5 mg/l2,4-D + 0.5 mg/l. More percentage shoot formation was obtained from the media supplemented with 1 mg/l 6-benzylaminopurine (BA). Average number of shoot per callus was also more in the media fortified with 1 mg/l BA (2.25) but this was not significantly different from what was obtained from the media fortified with 2 mg/l BA+ 0.1 mg/l 2,4-D at 5% level of significance.

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Section: Callus Inductionmentioning

confidence: 95%

Optimum Protocol for Shoot Formation in karandafi Red Sorghum (<i>Sorghum bicolor</i> (L.) Moench) through Somatic Embryogenesis Using Mature Embryo

.¹,

Usman²,

Katung³

et al. 2014

AJPS

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Genetic Transformation for Functional Genomics of Sorghum

Dalal

2016

Compendium of Plant Genomes

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Penetrance, expressivity and inheritance of axillary branching in somaclonal mutant of sorghum (Sorghum bicolor L.)

et al. 2014

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Untitled

Cited by 15 publications

References 7 publications

Optimum Protocol for Shoot Formation in karandafi Red Sorghum (<i>Sorghum bicolor</i> (L.) Moench) through Somatic Embryogenesis Using Mature Embryo

Optimum Protocol for Shoot Formation in karandafi Red Sorghum (<i>Sorghum bicolor</i> (L.) Moench) through Somatic Embryogenesis Using Mature Embryo

Genetic Transformation for Functional Genomics of Sorghum

Penetrance, expressivity and inheritance of axillary branching in somaclonal mutant of sorghum (Sorghum bicolor L.)

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Untitled

Cited by 15 publications

References 7 publications

Optimum Protocol for Shoot Formation in karandafi Red Sorghum (&lt;i&gt;Sorghum bicolor&lt;/i&gt; (L.) Moench) through Somatic Embryogenesis Using Mature Embryo

Optimum Protocol for Shoot Formation in karandafi Red Sorghum (&lt;i&gt;Sorghum bicolor&lt;/i&gt; (L.) Moench) through Somatic Embryogenesis Using Mature Embryo

Genetic Transformation for Functional Genomics of Sorghum

Penetrance, expressivity and inheritance of axillary branching in somaclonal mutant of sorghum (Sorghum bicolor L.)

Contact Info

Product

Resources

About

Optimum Protocol for Shoot Formation in karandafi Red Sorghum (<i>Sorghum bicolor</i> (L.) Moench) through Somatic Embryogenesis Using Mature Embryo

Optimum Protocol for Shoot Formation in karandafi Red Sorghum (<i>Sorghum bicolor</i> (L.) Moench) through Somatic Embryogenesis Using Mature Embryo