“…Standard cultures of murine fibroblast cells (L929) and pheochromocytoma cells (PC12), obtained from Marinpharm (Luckenwalde, Germany) were performed as described in detail elsewhere [13,14]. In both cases, prior to cell inoculation and during cell culture on modified polymeric substrates, cell culture medium was supplemented with 1 mg/ml pluronic F-68 [15].…”
Section: Cell Culture and Surface Conditioning With Proteinsmentioning
“…Standard cultures of murine fibroblast cells (L929) and pheochromocytoma cells (PC12), obtained from Marinpharm (Luckenwalde, Germany) were performed as described in detail elsewhere [13,14]. In both cases, prior to cell inoculation and during cell culture on modified polymeric substrates, cell culture medium was supplemented with 1 mg/ml pluronic F-68 [15].…”
Section: Cell Culture and Surface Conditioning With Proteinsmentioning
“…The mechanisms of attachment of cells to a wide variety of materials surfaces is currently of immense interest for the development of biomedical devices and sensors [1,2]. For in vitro cell culture the adsorption of serum proteins by the growth substrate is usually a precursor step to the attachment of the actual cells and the position, concentration and conformation of these proteins therefore plays a significant part in the attachment process.…”
“…Furthermore, DUV modification of methylmethacrylate polymers leads to a surface chemistry affecting the selective absorption of proteins and the adhesion of living cells in vitro. 26 The bifunctionality of the modified polymer chips supporting waveguides and cell anchorage capabilities at the same time provides the opportunity to monitor protein adsorption, cell attachment, and spreading processes by evanescent-field techniques. This will open up the possibility to develop cheap, disposable integrated optical sensors for biological monitoring.…”
Deep UV-induced modification of the refractive index of polymers is a useful technique for realizing integrated optical circuits for telecommunication and sensor applications. The combination with replication techniques such as hot embossing opens up a variety of possibilities such as a monolithic integration in optical or fluidic microsystems. In this article the fundamental understanding of the deep UV process is given including examples of fabricated waveguide based devices.
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