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Prediction of the Preservation of Lyophilized Tobamoviruses

“…3, y 4 = -0.000034906x +2, where y 4 is the survival (log 10 %) at 4 ºC and x is the time in days. When y 4 = 1 (log 10 The predictions for the longevity of the freeze-dried tobamovirus strains in different lyophilic forms are summarized in Table. The values for unprotected sap and leaves were obtained after accelerated ageing at 28-45 ºC analogously to the model developed for ToMV YM-0 (10). For strain ToMV GM-1 that was also unstable, the calculations were made for protected sap and preparation as well.…”

“…The calculations were described in details for ToMV YM-0 in a previous work (10). The Arrhenius equation in logarithmic form was used: log 10 K = log 10 K 0 -E/2.303R x 1/T, where K is the reaction rate, K 0 -specific constant of the reaction, E -energy of activation, R -gas constant and T -absolute temperature.…”

“…They have survived in dried leaves and lyophilized sap for decades (3,6). In several our previous works the preservation of different tobamovirus strains freeze-dried in the form of leaf material, plant sap and purified preparations was investigated by means of an accelerated storage test at elevated temperatures (7,8,9,10). Strain specific differences were observed and the most stable strain survived more than 5 times longer than the most labile one.…”

“…For a given strain, the preservation in leaves and unprotected sap was comparatively equal as well as this in protected sap and protected purified preparation. The worst preserved in lyophilized state strain of tomato mosaic virus (ToMV) was used to develop a model for predicting the stability of tobamoviruses based on the accelerated ageing and the application of Arrhenius equation (10).…”