LLIN evaluation in Uganda project (LLINEUP): The fabric integrity, chemical content and bioefficacy of long-lasting insecticidal nets treated with and without piperonyl butoxide across two years of operational use in Uganda

Mechan, Frank; Katureebe, Agaba; Tuhaise, Violet; Mugote, Martin; Oruni, Ambrose; Onyige, Ismail; Bumali, Kawesa; Thornton, Jonathan; Kilama, Maxwell; Kyohere, Mary; Kamya, Moses R.; Mutungi, Peter; Kigozi, Simon P.; Yeka, Adoke; Opigo, Jimmy; Maiteki‐Sebuguzi, Catherine; Gonahasa, Samuel; Hemingway, Janet; Dorsey, Grant; Reimer, Lisa J; Staedke, Sarah G.; Donnelly, Martin; Lynd, Amy

doi:10.1016/j.crpvbd.2022.100092

Cited by 21 publications

(24 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The efficacy of Olyset TM Plus decreased with net age and this correlates with the depletion of permethrin and PBO content after 36 months of community use (8.3g/kg vs 20g/kg when new and ∼0.7g/kg vs 10g/kg respectively). Similar findings were reported in Uganda (32) with low retention (3.7g/kg) of PBO content, 25 months post distribution for Olyset TM Plus LLIN and Kenya (33) 87% of PBO and 52% of permethrin were lost after 36 months of community use, while PBO retention was higher when nets were washed 20 times in the laboratory (2.0g/kg) in Benin (34).…”

Section: Discussionsupporting

confidence: 82%

Bio-efficacy of field aged novel class of long-lasting insecticidal nets, against pyrethroid-resistant malaria vectors in Tanzania: A series of experimental hut trials

Martin,

Messenger,

Rowland

et al. 2023

Preprint

View full text Add to dashboard Cite

New classes of long-lasting insecticidal nets (LLINs) incorporating two insecticides, or an insecticide and a synergist, are recommended by the World Health Organization (WHO) to prevent malaria transmitted by mosquito vectors resistant to pyrethroid and other common insecticide classes. This study was nested in a large-scale cluster-randomized controlled trial conducted in Tanzania. A series of experimental hut trials (EHTs). The following field collected LLINs were assessed: 1/OlysetTM Plus (combining piperonyl butoxide synergist and permethrin), 2/Interceptor® G2 (chlorfenapyr and alpha-cypermethrin), 3/Royal Guard® (pyriproxyfen and alpha-cypermethrin), 4/Interceptor® (alpha-cypermethrin only), 5/a new Interceptor®, and 6/an untreated net. Thirty nets of each type were withdrawn from the community at 12, 24 and 36 months after distribution and used for the EHTs. Pre-specified outcomes were 72-hour mortality for Interceptor® G2, 24-hour mortality for OlysetTM Plus, and fertility based on egg development stage for Royal Guard®. Overall; Interceptor® G2 LLINs induced higher 72-hour mortality compared to standard LLINs of the same age up to 12 months (44% vs 21%), OR: 3.5, 95% CI: 1.9 – 6.6, p-value < 0.001 and 24-hour mortality was only significantly higher in OlysetTM Plus when new (OR: 13.6, 95%CI: 4.4 – 41.3, p-value < 0.001) compared to standard LLINs but not at 12 months (17% vs 13%; OR: 2.1, 95% CI: 1.0 – 4.3; p-value = 0.112). A small non-significant effect of pyriproxyfen on Anopheles fertility was observed for Royal Guard® up to 12 months (75% vs 98%, OR: 1.1, 95% CI: 0.0 – 24.9, p-value = 0.951). There was no evidence of a difference in the main outcomes for any of the new class of LLINs at 24 and 36 months compared to standard LLINs. Interceptor® G2 LLINs showed superior bio-efficacy compared to standard LLINs for only up to 12 months and the effect of OlysetTM Plus was observed when new for all species and 12 months for An. gambiae s.l. only. The pyriproxyfen component of Royal Guard® had a short and limited effect on fertility

show abstract

Section: Discussionsupporting

confidence: 82%

Bio-efficacy of field aged novel class of long-lasting insecticidal nets, against pyrethroid-resistant malaria vectors in Tanzania: A series of experimental hut trials

Martin,

Messenger,

Rowland

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…As part of the LLINEUP trial, we withdrew LLINs from the community at 12 months and 25 months after LLIN distribution, assayed the chemical content of the nets (pyrethroid insecticide and PBO), and conducted bioassays on the same nets with pyrethroid-resistant mosquitoes. 17,20 We observed that that the concentration of PBO on the net was strongly predictive of mosquito mortality. These results have been published separately.…”

Section: Discussionmentioning

confidence: 88%

“…These results have been published separately. 20 Limitations to the design of this pragmatic trial embedded with a LLIN distribution campaign led by the Ugandan Ministry of Health have been discussed. 12 Additional limitations were present in the current study.…”

Section: Discussionmentioning

confidence: 99%

Effect of long-lasting insecticidal nets with and without piperonyl butoxide on malaria indicators in Uganda (LLINEUP): final results of a cluster-randomised trial embedded in a national distribution campaign

Maiteki‐Sebuguzi¹,

Gonahasa²,

Kamya³

et al. 2023

The Lancet Infectious Diseases

View full text Add to dashboard Cite

“…Fourth, the concentration of PBO used was 4.0% which may not be directly comparable to the concentration of PBO on LLINs. In a study of PBO LLINs distributed by the Ugandan Ministry of Health in 2017–2018, the concentration of PBO at baseline was 26.81 g/kg in PermaNet 3.0, and 8.17 g/kg in Olyset Plus ( Mechan et al., 2022 ) which may not be equivalent to the concentration included in the WHO tube assay. Finally, the absence of historical data before LLIN and/or IRS implementation limited the inferences that could be made on the development and spread of pyrethroid resistance mutations.…”

Section: Discussionmentioning

confidence: 99%

Characterizing pyrethroid resistance and mechanisms in Anopheles gambiae (s.s.) and Anopheles arabiensis from 11 districts in Uganda

Mawejje¹,

Weetman²,

Epstein³

et al. 2023

Current Research in Parasitology & Vector-Borne Diseases

View full text Add to dashboard Cite

LLIN evaluation in Uganda project (LLINEUP): The fabric integrity, chemical content and bioefficacy of long-lasting insecticidal nets treated with and without piperonyl butoxide across two years of operational use in Uganda

Cited by 21 publications

References 32 publications

Bio-efficacy of field aged novel class of long-lasting insecticidal nets, against pyrethroid-resistant malaria vectors in Tanzania: A series of experimental hut trials

Bio-efficacy of field aged novel class of long-lasting insecticidal nets, against pyrethroid-resistant malaria vectors in Tanzania: A series of experimental hut trials

Effect of long-lasting insecticidal nets with and without piperonyl butoxide on malaria indicators in Uganda (LLINEUP): final results of a cluster-randomised trial embedded in a national distribution campaign

Characterizing pyrethroid resistance and mechanisms in Anopheles gambiae (s.s.) and Anopheles arabiensis from 11 districts in Uganda

Contact Info

Product

Resources

About