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Sj, Utsalo; Eko, Francis O.; Umoh, F.; Aa, Asindi

doi:10.1023/a:1007541317104

Cited by 14 publications

(3 citation statements)

References 7 publications

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“…Furthermore, considering that the V . cholerae load in stools from convalescent and long-term carriers is reportedly in the range of 10 2 to 10 3 CFU/g [ 43 , 44 ] the method is also valuable for the bacterium detection in these samples.…”

Section: Resultsmentioning

confidence: 99%

gbpA as a Novel qPCR Target for the Species-Specific Detection of Vibrio cholerae O1, O139, Non-O1/Non-O139 in Environmental, Stool, and Historical Continuous Plankton Recorder Samples

et al. 2015

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The Vibrio cholerae N-acetyl glucosamine-binding protein A (GbpA) is a chitin-binding protein involved in V. cholerae attachment to environmental chitin surfaces and human intestinal cells. We previously investigated the distribution and genetic variations of gbpA in a large collection of V. cholerae strains and found that the gene is consistently present and highly conserved in this species. Primers and probe were designed from the gbpA sequence of V. cholerae and a new Taq-based qPCR protocol was developed for diagnostic detection and quantification of the bacterium in environmental and stool samples. In addition, the positions of primers targeting the gbpA gene region were selected to obtain a short amplified fragment of 206 bp and the protocol was optimized for the analysis of formalin-fixed samples, such as historical Continuous Plankton Recorder (CPR) samples. Overall, the method is sensitive (50 gene copies), highly specific for V. cholerae and failed to amplify strains of the closely-related species Vibrio mimicus. The sensitivity of the assay applied to environmental and stool samples spiked with V. cholerae ATCC 39315 was comparable to that of pure cultures and was of 102 genomic units/l for drinking and seawater samples, 101 genomic units/g for sediment and 102 genomic units/g for bivalve and stool samples. The method also performs well when tested on artificially formalin-fixed and degraded genomic samples and was able to amplify V. cholerae DNA in historical CPR samples, the earliest of which date back to August 1966. The detection of V. cholerae in CPR samples collected in cholera endemic areas such as the Benguela Current Large Marine Ecosystem (BCLME) is of particular significance and represents a proof of concept for the possible use of the CPR technology and the developed qPCR assay in cholera studies.

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Section: Resultsmentioning

confidence: 99%

gbpA as a Novel qPCR Target for the Species-Specific Detection of Vibrio cholerae O1, O139, Non-O1/Non-O139 in Environmental, Stool, and Historical Continuous Plankton Recorder Samples

et al. 2015

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“…Mathematical modeling suggests that an association between HIV and cholera may also exist in the endemic setting [ 15 ]. Beyond this, evidence is limited to several small case series [ 16 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

Risk Factors for Self-Reported Cholera Within HIV-Affected Households in Rural Haiti

Richterman

Cheung

Meiselbach

et al. 2018

Open Forum Infectious Diseases

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BackgroundCholera continues to be a major cause of morbidity and mortality worldwide and is now endemic in Haiti since first being introduced in 2010. Cholera and HIV have significant geographic overlap globally, but little is known about the clinical features and risk of cholera among HIV-infected people and their households.MethodsWe assessed HIV-affected households originally recruited for a randomized controlled trial of food supplements. We assessed for correlation between household and individual factors and reported history of cholera since 2010 using univariable and multivariable analyses.ResultsThere were 352 HIV-infected household members, 32 with reported history of medically attended cholera, and 1968 other household members, 55 with reported history of medically attended cholera. Among HIV-infected individuals in this study, no variables correlated with reported history of cholera in univariable analyses. Among all household members, known HIV infection (adjusted odds ratio [AOR], 3.75; 95% CI, 2.43–5.79; P < .0001), source of income in the household (AOR, 1.82; 95% CI, 1.05–3.15; P = .034), time required to fetch water (AOR, 1.07 per 5-minute increase; 95% CI, 1.01–1.12; P = .015), and severe household food insecurity (AOR, 3.23; 95% CI, 1.25–8.34; P = .016) were correlated with reported history of cholera in a multivariable analysis.ConclusionsKnown HIV infection, source of household income, time required to fetch water, and severe household food insecurity were independently associated with reported history of medically attended cholera in HIV-affected households in rural Haiti. Further research is required to better understand the interactions between HIV and cholera.

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“…A study from Nigeria followed 13 cholera convalescent patients with twice weekly stool cultures and rectal swabs and showed that 69% of the convalescents had positive cholera faecal cultures for periods ranging from two weeks to more than seven months [45]. However, as in all endemic-region studies, reinfection rather than long-term shedding could not be discounted.…”

Section: Choleramentioning

confidence: 99%

From Haiti to the Amazon: Public Health Issues Related to the Recent Immigration of Haitians to Brazil

et al. 2014

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Untitled

Cited by 14 publications

References 7 publications

gbpA as a Novel qPCR Target for the Species-Specific Detection of Vibrio cholerae O1, O139, Non-O1/Non-O139 in Environmental, Stool, and Historical Continuous Plankton Recorder Samples

gbpA as a Novel qPCR Target for the Species-Specific Detection of Vibrio cholerae O1, O139, Non-O1/Non-O139 in Environmental, Stool, and Historical Continuous Plankton Recorder Samples

Risk Factors for Self-Reported Cholera Within HIV-Affected Households in Rural Haiti

From Haiti to the Amazon: Public Health Issues Related to the Recent Immigration of Haitians to Brazil

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