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Calvo, Pilar; Remuñán‐López, Carmen; Vila-Jato, J. L.; Alonso, Marı́a José

doi:10.1023/a:1012128907225

Cited by 735 publications

(341 citation statements)

References 6 publications

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“…Therefore, using the optimized operating parameters, this simple and solvent-free procedure was chosen to prepare the NPs in this work. The 5:1 chitosan to TPP ratio was confirmed as a stable and suitable ratio to carry out the following studies, as also done by Calvo et al (1997). Decreasing the chitosan to TPP ratio provokes an increasing turbidity, indicating a shift in the size variation of the particles to the larger end of the scale.…”

Section: Resultsmentioning

confidence: 53%

“…Nanoparticle preparation CS-NPs were spontaneously obtained by ionotropic gelation technique, according to the methodology previously developed by Calvo et al (1997) …”

Section: Surfactant Included In the Nanoparticlesmentioning

confidence: 99%

“…This is possibly due to the drug molecules dispersing close to the NP surface. Considering the electrostatic attraction existing between the negatively charged drug and the positively charged polymer, we can assume that the MTX molecules are both adsorbed at the particle surface (Gan and Wang 2007) and entrapped/embedded into CS nano-matrix by hydrogen bonding and hydrophobic forces (Calvo et al 1997). Therefore, the way by which the drug is associated with the carrier (adsorption or encapsulation) might determine its release rate from the matrix.…”

Section: In Vitro Release Studymentioning

confidence: 99%

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Inclusion of a pH-responsive amino acid-based amphiphile in methotrexate-loaded chitosan nanoparticles as a delivery strategy in cancer therapy

et al. 2015

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The encapsulation of antitumor drugs in nanosized systems with pH-sensitive behavior is a promissing approach that may enhance the success of chemotherapy in many cancers. The nanocarrier dependence on pH might trigger an efficient delivery of the encapsulated drug both in the acidic extracellular environment of tumors and, especially, in the intracellular compartments through disruption of endosomal membrane. In this context, here we reported the preparation of chitosan-based nanoparticles encapsulating methotrexate as a model drug (MTX-CS-NPs), which comprises the incorporation of an amino acid-based amphiphile with pHresponsive properties (77KS) on the ionotropic complexation process. The presence of 77KS clearly gives a pH-sensitive behavior to NPs, which allowed accelerated release of MTX with decreasing pH as well as pH-dependent membrane-lytic activity. This latter performance demonstrates the potential of these NPs to facilitate cytosolic delivery of endocytosed materials.Outstandingly, the cytotoxicity of MTX-loaded CS-NPs was higher than free drug to MCF-7 tumor cells and, to a lesser extent, to HeLa cells. Based on the overall results, MTX-CS-NPs modified with the pH-senstive surfactant 77KS could be potentially useful as a carrier system for intracellular drug delivery and, thus, a promising targeting anticancer chemotherapeutic agent.

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Section: Resultsmentioning

confidence: 53%

“…Nanoparticle preparation CS-NPs were spontaneously obtained by ionotropic gelation technique, according to the methodology previously developed by Calvo et al (1997) …”

Section: Surfactant Included In the Nanoparticlesmentioning

confidence: 99%

Section: In Vitro Release Studymentioning

confidence: 99%

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Inclusion of a pH-responsive amino acid-based amphiphile in methotrexate-loaded chitosan nanoparticles as a delivery strategy in cancer therapy

et al. 2015

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“…At a subsequent stage, MS-MPs were modified through the deposition of a chitosan (CS) coating, thus forming a multifunctional silicon/polymer composite with potential for advanced biomedical applications, a concept that has been recently explored in some recent works [23,24]. The rational behind the selection of CS as the coating polymer was: (i) its know capacity to adsorb to polyactionic surfaces, (ii) its established pharmaceutical record, (iii) and its mucoadhesivess and permeation enhancing properties [25][26][27][28]. The global concept was to generate a drug delivery system that would combine silicon drug loading properties with the unique characteristics of CS-coated systems for transmucoal drug delivery.…”

Section: Introductionmentioning

confidence: 99%

Protein delivery based on uncoated and chitosan-coated mesoporous silicon microparticles

Pastor

Matveeva

Valle-Gallego

et al. 2011

Colloids and Surfaces B: Biointerfaces

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Mesoporous silicon is a biocompatible, biodegradable material that is receiving increased attention for pharmaceutical applications due to its extensive specific surface. This feature enables to load a variety of drugs in mesoporous silicon devices by simple adsorption-based procedures. In this work, we have addressed the fabrication and characterization of two new mesoporous silicon devices prepared by electrochemistry and intended for protein delivery, namely: (i) mesoporous silicon microparticles and (ii) chitosan-coated mesoporous silicon microparticles. Both carriers were investigated for their capacity to load a therapeutic protein (insulin) and a model antigen (bovine serum albumin) by adsorption. Our results show that mesoporous silicon microparticles prepared by electrochemical methods present moderate affinity for insulin and high affinity for albumin. However, mesoporous silicon presents an extensive capacity to load both proteins, leading to systems were protein could represent the major mass fraction of the formulation. The possibility to form a chitosan coating on the microparticles surface was confirmed both qualitatively by atomic force microscopy and quantitatively by a colorimetric method.Mesoporous silicon microparticles with mean pore size of 35 nm released the loaded insulin quickly, but not instantaneously. This profile could be slowed to a certain extent by the chitosan coating modification. With their high protein loading, their capacity to provide a controlled release of insulin over a period of 60-90 min, and the potential mucoadhesive effect of the chitosan coating, these composite devices comprise several features that render them interesting candidates as transmucosal protein delivery systems.

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“…82 Para tanto, o polímero é misturado com agentes reticulantes iônicos (geralmente sais com alta densidade eletrônica como tripolifosfato ou sulfato de sódio), havendo uma transição sol-gel do polímero, conjuntamente com uma nanoprecipitação controlada. 83 Ao contrário de sistemas PEGuilados, onde há uma maior penetração das nanopartículas, para o caso de nanopartículas com quitosana parece haver um maior depósito na superfície ocular. 84 Entretanto, lipossomas com nanopartículas de quitosana encapsuladas foram capazes de ser internalizados por células de conjuntiva humana (IOBA-NHC).…”

Section: Polímeros Catiônicos Quitosanaunclassified

Biomateriais para formulações de base nanotecnológica visando terapia gênica ocular

et al. 2016

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Abstract: CS and CS/PEO-PPO nanoparticles prepared by a very mild ionic crosslinking technique are novel and suitable systems for the entrapment and controlled release of proteins and vaccines.

Cited by 735 publications

References 6 publications

Inclusion of a pH-responsive amino acid-based amphiphile in methotrexate-loaded chitosan nanoparticles as a delivery strategy in cancer therapy

Inclusion of a pH-responsive amino acid-based amphiphile in methotrexate-loaded chitosan nanoparticles as a delivery strategy in cancer therapy

Protein delivery based on uncoated and chitosan-coated mesoporous silicon microparticles

Biomateriais para formulações de base nanotecnológica visando terapia gênica ocular

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