LKB1 loss promotes colorectal cancer cell metastasis through regulating TNIK expression and actin cytoskeleton remodeling

Abstract

Colorectal cancer (CRC) is one of the most common malignant tumors. Approximately 5%–6% of CRC cases are associated with hereditary CRC syndromes, including the Peutz–Jeghers syndrome (PJS). Liver kinase B1 (LKB1), also known as STK11, is the major gene responsible for PJS. LKB1 heterozygotic deficiency is involved in intestinal polyps in mice, while the mechanism of LKB1 in CRC remains elusive. In this study, we generated LKB1 knockout (KO) CRC cell lines by using CRISPR‐Cas9. LKB1 KO promoted CRC cell motility in vitro and tumor metastases in vivo. LKB1 attenuated expression of TRAF2 and NCK‐interacting protein kinase (TNIK) as accessed by RNA‐seq and western blots, and similar suppression was also detected in the tumor tissues of azoxymethane/dextran sodium sulfate‐induced intestinal‐specific LKB1‐KO mice. LKB1 repressed TNIK expression through its kinase activity. Moreover, attenuating TNIK by shRNA inhibited cell migration and invasion of CRC cells. LKB1 loss‐induced high metastatic potential of CRC cells was depended on TNIK upregulation. Furthermore, TNIK interacted with ARHGAP29 and further affected actin cytoskeleton remodeling. Taken together, LKB1 deficiency promoted CRC cell metastasis via TNIK upregulation and subsequently mediated cytoskeleton remodeling. These results suggest that LKB1‐TNIK axis may play a crucial role in CRC progression.

B7-H3 promotes the migration and invasion of colorectal cancer cells via regulating the actin cytoskeleton and RhoA/ROCK1/LIMK1 signaling pathway

“…For instance, leucine zipper tumor suppressor family member 3 (LZTS3) regulated CRC cell proliferation, migration, and actin cytoskeleton, which inhibited the progression of CRC [22]. A study by Guanghui Hu et al showed that the loss of liver kinase B1 (LKB1) facilitated tumor cell metastasis in CRC via the upregulation of TRAF2 and NCK-interacting protein kinase (TNIK) and subsequently induced cytoskeleton remodeling [23]. In addition, phospholipase C Beta 1 (PLCB1) depletion resulted in a decrease in gastric cancer cell migration, and invasion by modulating actin cytoskeletal remodeling and EMT [24].…”